FastQCFastQC Report
Fri 17 Jun 2016
SRR1527831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299231
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC825991.5586978563493459No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG447080.8436695814921071No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC208550.3935476675766729No Hit
CGCTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT155650.29372186266271466No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT144480.2726433325892002No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC108200.20418056884102617No Hit
CGCTTGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA101050.19068804511447038No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT90710.1711757800329897No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA71980.1358310290681799No Hit
CGCTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG63780.12035708577338862No Hit
CGCTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGC56350.10633618349530337No Hit
CGCTTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC56050.10577006361866467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTGG5479700.042.3121721
GCTTGGG5456000.042.188712
CTTGGGG5092800.042.1035963
TTGGGGG2533150.041.835744
TGGGGGG1167900.041.6792535
TTGGGGA2018150.041.2409364
TGGGGGA601700.040.844615
TGGGGAG661150.040.7190485
TTGGGGC360700.040.6698074
TGGGGGT438600.040.5891465
TGGGGAC372200.040.4357875
TGGGGGC371000.040.216715
TTGGGGT262350.040.2096444
CTTGGGA208300.039.912633
TGGGGAA332950.039.6654135
TGGGGAT716950.039.1271365
TTGGGTA48300.038.94414
TGGGGTA73900.037.450615
TGGGGCA139850.037.2985345
TTGGGAT65550.037.2875674