##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5299231
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.661486921404254	34.0	31.0	34.0	31.0	34.0
2	33.00194160247025	34.0	31.0	34.0	31.0	34.0
3	33.20586251099452	34.0	34.0	34.0	31.0	34.0
4	36.52089123874766	37.0	37.0	37.0	35.0	37.0
5	36.59359046623935	37.0	37.0	37.0	35.0	37.0
6	36.411360818201736	37.0	37.0	37.0	35.0	37.0
7	36.65740274390756	37.0	37.0	37.0	35.0	37.0
8	36.720839872804184	37.0	37.0	37.0	35.0	37.0
9	38.67200278681945	39.0	39.0	39.0	39.0	39.0
10	38.20602310033286	39.0	39.0	39.0	37.0	39.0
11	37.171173326846855	39.0	35.0	39.0	35.0	39.0
12	36.96142968668473	39.0	35.0	39.0	34.0	39.0
13	36.88307246843929	39.0	35.0	39.0	33.0	39.0
14	37.57917384616749	40.0	35.0	40.0	33.0	40.0
15	37.658765394450626	40.0	35.0	40.0	34.0	40.0
16	37.71275907768504	40.0	35.0	40.0	34.0	40.0
17	37.51516399266233	40.0	35.0	40.0	33.0	40.0
18	37.479576187563815	40.0	35.0	40.0	33.0	40.0
19	37.46699266365252	40.0	35.0	40.0	33.0	40.0
20	37.35517058984596	40.0	35.0	40.0	33.0	40.0
21	37.35714842398831	40.0	35.0	40.0	33.0	40.0
22	37.416755751919474	40.0	35.0	40.0	33.0	40.0
23	37.41678292567356	40.0	35.0	40.0	33.0	40.0
24	37.40863117686321	40.0	35.0	40.0	34.0	40.0
25	37.380840352119016	40.0	35.0	40.0	34.0	40.0
26	37.304932168459914	39.0	35.0	40.0	34.0	40.0
27	37.22641398346288	39.0	35.0	40.0	33.0	40.0
28	37.072916617524314	39.0	35.0	40.0	33.0	40.0
29	37.16617901729515	40.0	35.0	40.0	33.0	40.0
30	37.279207869972076	40.0	35.0	40.0	33.0	40.0
31	37.31167465619068	40.0	35.0	40.0	34.0	40.0
32	37.31647818334396	40.0	35.0	40.0	34.0	40.0
33	37.24793050161429	40.0	35.0	40.0	33.0	40.0
34	36.83237850925917	40.0	35.0	40.0	32.0	40.0
35	36.84946098783012	40.0	35.0	40.0	32.0	40.0
36	36.9274358864522	39.0	35.0	40.0	33.0	40.0
37	36.914155091559515	39.0	35.0	40.0	33.0	40.0
38	36.86107059684698	39.0	35.0	40.0	33.0	40.0
39	36.764400344125406	39.0	35.0	40.0	33.0	40.0
40	36.62483820765692	39.0	35.0	40.0	33.0	40.0
41	36.5123124468437	39.0	35.0	40.0	33.0	40.0
42	36.45564196012591	39.0	35.0	40.0	32.0	40.0
43	36.38897889146557	38.0	35.0	40.0	32.0	40.0
44	36.12200468332103	38.0	35.0	40.0	31.0	40.0
45	36.09512550028485	38.0	35.0	40.0	31.0	40.0
46	35.96303859937414	37.0	35.0	40.0	31.0	40.0
47	35.8823516091297	37.0	35.0	40.0	31.0	40.0
48	35.820131071847975	37.0	35.0	40.0	31.0	40.0
49	35.78141035935214	37.0	35.0	40.0	31.0	40.0
50	35.31086585959359	36.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	26.0
15	57.0
16	204.0
17	466.0
18	1007.0
19	1856.0
20	2946.0
21	4421.0
22	6523.0
23	9463.0
24	13420.0
25	18698.0
26	24726.0
27	32125.0
28	41769.0
29	55492.0
30	73940.0
31	96216.0
32	126728.0
33	189935.0
34	542176.0
35	502351.0
36	334280.0
37	599800.0
38	1500851.0
39	1119746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.42619976370156	0.4465931000177195	0.09501378596252928	0.03219335031818767
8	99.22217016016097	0.602596867356792	0.08355929379187281	0.09167367869036093
9	93.17142430665884	3.5399664592843756	2.008725416952007	1.2798838171047837
10	48.499169030374404	38.083884246601066	6.178972005560807	7.237974717463723
11	38.131174126962954	21.260367777890792	24.43811186943917	16.17034622570709
12	34.226362277847485	16.655643054624342	24.53210663962375	24.585888027904428
13	26.779451584578972	17.03920814170962	27.538184313912716	28.643155959798694
14	25.647362796601996	19.018174523812984	29.53668183176012	25.797780847824903
15	28.458147984113168	20.39888429094712	26.875559868969667	24.267407855970045
16	34.3674582217684	21.09628736697834	23.356011466569395	21.18024294468386
17	31.143575360273974	21.193320313834217	24.71590689290578	22.94719743298603
18	30.558301761142324	20.80086714468571	24.704810943323665	23.936020150848304
19	29.015398649351198	25.031179052205875	23.078820304304532	22.874601994138395
20	30.925958879694054	23.902713431439395	23.342669908143275	21.82865778072328
21	29.703347523442552	24.031052807473387	24.596059315021368	21.669540354062693
22	27.056680488168944	21.55544832825744	27.706397399924633	23.681473783648986
23	28.377324936391712	21.743456739289154	26.884429080370342	22.99478924394879
24	30.126880673818523	21.982114008617476	24.002369400390357	23.88863591717364
25	27.888688000202293	23.143716512829883	22.745941816840972	26.221653670126855
26	25.068241033463156	23.630164452162965	25.827955037249744	25.47363947712413
27	24.692092116761845	21.092135821216324	25.19046631482945	29.025305747192377
28	25.29980293367094	24.919804401808488	23.498956735420666	26.2814359290999
29	29.39807681529641	24.056641425897457	22.297405038580127	24.24787672022601
30	28.140516991993742	21.63349738858336	25.929026305892304	24.296959313530586
31	30.539449969250253	21.946203137776028	24.345192727020205	23.16915416595351
32	32.43357385250803	22.13713650150371	23.19734693581012	22.23194271017814
33	32.498073777119735	21.918123591894748	23.3948095487817	22.188993082203815
34	27.437377234545917	23.106748884885373	28.102417124295958	21.35345675627275
35	25.23996406271023	27.74015701523485	25.99630021789954	21.023578704155376
36	25.730299358529567	29.244847035352866	23.318270141460147	21.70658346465742
37	28.95820167114814	26.796906947441997	21.563204170567392	22.68168721084248
38	28.04744688427434	27.890197653206663	21.944070752907354	22.11828470961164
39	29.776339246203836	24.851681309986297	22.085940393993013	23.28603904981685
40	27.509991544056113	23.854027122048464	21.992926898261274	26.64305443563415
41	24.258916057820464	26.822325729903074	22.440010635505416	26.478747576771045
42	23.42734257102587	24.249650562506144	23.332555233014	28.990451633453983
43	23.717252559852554	22.870563672351704	24.284561288232197	29.12762247956354
44	24.995777689253405	22.661344636608593	23.766316282494575	28.57656139164343
45	27.4813836196233	23.725178238125494	22.847050826808648	25.94638731544256
46	25.871225466487495	26.71472521201661	22.277760678860766	25.13628864263513
47	23.948965425360775	25.09118775912958	24.52684172477101	26.433005090738636
48	25.17025583523345	23.505184054063694	26.63478908543523	24.68977102526763
49	28.403423062704757	22.905134726151775	24.517783806744788	24.173658404398676
50	24.77000908244989	23.59472534788538	24.34889137688091	27.286374192783818
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.5
8	7.0
9	75.5
10	144.0
11	334.0
12	524.0
13	2348.0
14	4172.0
15	8667.5
16	13163.0
17	15676.5
18	18190.0
19	15636.0
20	13082.0
21	13195.0
22	13308.0
23	14467.5
24	15627.0
25	15926.0
26	16225.0
27	19117.5
28	22010.0
29	25545.5
30	29081.0
31	34620.0
32	40159.0
33	48432.5
34	56706.0
35	62902.5
36	69099.0
37	79811.0
38	90523.0
39	99069.5
40	107616.0
41	119884.5
42	132153.0
43	141133.0
44	150113.0
45	159693.5
46	169274.0
47	179599.5
48	189925.0
49	208454.0
50	226983.0
51	241555.5
52	256128.0
53	273718.5
54	291309.0
55	304357.0
56	317405.0
57	336677.5
58	355950.0
59	382931.0
60	409912.0
61	442436.5
62	474961.0
63	477473.5
64	479986.0
65	505189.5
66	530393.0
67	415944.0
68	301495.0
69	242777.5
70	184060.0
71	151894.0
72	119728.0
73	100803.5
74	81879.0
75	66036.5
76	50194.0
77	44967.5
78	39741.0
79	29104.5
80	18468.0
81	11876.5
82	5285.0
83	4266.0
84	3247.0
85	1975.5
86	704.0
87	443.0
88	182.0
89	133.5
90	85.0
91	53.0
92	21.0
93	15.0
94	9.0
95	7.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5299231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.046758154808779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.923148347929924	4.293106924374001
2	10.330250555293404	1.455895546834634
3	4.078782686956044	0.8622658548300111
4	1.914438070041833	0.5396232832777466
5	1.0298829166715748	0.3628667920776834
6	0.7355299809767439	0.310986113493253
7	0.5308762145190943	0.26186694056995097
8	0.45719746022260366	0.25774079449451953
9	0.3728110508611852	0.23643983815729983
>10	11.924301090137691	24.73170665644511
>50	5.186508802869059	25.07071691184241
>100	2.4051281720939377	28.71287898466649
>500	0.06772450504645107	3.157084695161033
>1k	0.03932390615600384	4.963893156687668
>5k	0.0021846614531113246	0.9515514080599949
>10k+	0.0019115787714724089	3.8313760990281707
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	82599	1.5586978563493459	No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	44708	0.8436695814921071	No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	20855	0.3935476675766729	No Hit
CGCTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	15565	0.29372186266271466	No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	14448	0.2726433325892002	No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	10820	0.20418056884102617	No Hit
CGCTTGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	10105	0.19068804511447038	No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	9071	0.1711757800329897	No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	7198	0.1358310290681799	No Hit
CGCTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	6378	0.12035708577338862	No Hit
CGCTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGC	5635	0.10633618349530337	No Hit
CGCTTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC	5605	0.10577006361866467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0013020757162690209	0.0	0.0	0.0	0.0
8	0.004547829675664261	0.0	0.0	0.0	0.0
9	0.013964290290421384	0.0	0.0	0.0	0.0
10	0.04087385509331448	0.0	0.0	0.0	0.0
11	0.053517199004912225	0.0	0.0	0.0	0.0
12	0.064103640698056	0.0	0.0	0.0	0.0
13	0.0706140192794011	0.0	0.0	0.0	0.0
14	0.08408767234340228	0.0	0.0	0.0	0.0
15	0.0972027828188656	0.0	0.0	0.0	0.0
16	0.1103745052819928	0.0	0.0	0.0	0.0
17	0.1229046252182628	0.0	0.0	0.0	0.0
18	0.13558571045496978	0.0	0.0	0.0	0.0
19	0.15228624681581157	0.0	0.0	0.0	0.0
20	0.1664581143943338	0.0	0.0	0.0	0.0
21	0.17849759710418361	0.0	0.0	0.0	0.0
22	0.1887821081964534	0.0	0.0	0.0	0.0
23	0.20133109879527802	0.0	0.0	0.0	0.0
24	0.2134460641553463	0.0	0.0	0.0	0.0
25	0.2253345815647591	0.0	0.0	0.0	0.0
26	0.23807227878912998	0.0	0.0	0.0	0.0
27	0.24943241764701332	0.0	0.0	0.0	0.0
28	0.26383073317619105	0.0	0.0	0.0	0.0
29	0.2749832947459735	0.0	0.0	0.0	0.0
30	0.2875134146822435	0.0	0.0	0.0	0.0
31	0.29979821600530343	0.0	0.0	0.0	0.0
32	0.3113093201636237	0.0	0.0	0.0	0.0
33	0.3218768911942129	0.0	0.0	0.0	0.0
34	0.3342560458300459	0.0	0.0	0.0	0.0
35	0.3492582225609716	0.0	0.0	0.0	0.0
36	0.3652228030821831	0.0	0.0	0.0	0.0
37	0.37920596403515905	0.0	0.0	0.0	0.0
38	0.39186817860931145	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTGG	547970	0.0	42.312172	1
GCTTGGG	545600	0.0	42.18871	2
CTTGGGG	509280	0.0	42.103596	3
TTGGGGG	253315	0.0	41.83574	4
TGGGGGG	116790	0.0	41.679253	5
TTGGGGA	201815	0.0	41.240936	4
TGGGGGA	60170	0.0	40.84461	5
TGGGGAG	66115	0.0	40.719048	5
TTGGGGC	36070	0.0	40.669807	4
TGGGGGT	43860	0.0	40.589146	5
TGGGGAC	37220	0.0	40.435787	5
TGGGGGC	37100	0.0	40.21671	5
TTGGGGT	26235	0.0	40.209644	4
CTTGGGA	20830	0.0	39.91263	3
TGGGGAA	33295	0.0	39.665413	5
TGGGGAT	71695	0.0	39.127136	5
TTGGGTA	4830	0.0	38.9441	4
TGGGGTA	7390	0.0	37.45061	5
TGGGGCA	13985	0.0	37.298534	5
TTGGGAT	6555	0.0	37.287567	4
>>END_MODULE