FastQCFastQC Report
Fri 17 Jun 2016
SRR1527830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7936902
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1245371.569088291628144No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT583440.7350979009190236No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA317090.3995135633525524No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC221050.27850917146261855No Hit
CGCTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC212170.26732092698133353No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC162130.20427365740436254No Hit
CGCTTGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC149500.18836064751712947No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT136140.1715278832975385No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC107160.13501489624037188No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT102840.12957196649271968No Hit
CGCTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG93980.11840892076026642No Hit
CGCTTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT83260.10490239138646287No Hit
CGCTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTC82690.10418422704475878No Hit
CGCTTGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC81100.1021809265126368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTGG8201750.044.28351
GCTTGGG8164450.044.158472
CTTGGGG7631500.044.075373
TTGGGGG3802050.043.7974244
TGGGGGG1752050.043.4755865
TTGGGGA3027450.043.136634
TGGGGGA911450.042.8154035
TGGGGAG998550.042.4044845
TGGGGAC557350.042.331395
TGGGGGT657600.042.3100665
TTGGGGC532650.042.247634
TTGGGGT397600.042.1126754
TGGGGGC559250.042.0230675
TTGGGTA67950.041.3966144
TGGGGAA513750.041.1291475
TGGGGAT1063500.040.9285355
CTTGGGA307850.040.6282273
TGGGGTA110000.039.9154555
CCGTATA1350.039.1851946
TGGGGCA202150.039.1164975