FastQCFastQC Report
Fri 17 Jun 2016
SRR1527829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7998891
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1173201.4667033217479772No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT556840.6961465033090212No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA300280.3754020401078099No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC208460.2606111272175105No Hit
CGCTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC203130.25394770350039775No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC154830.19356433285564212No Hit
CGCTTGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC141530.176937027895492No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT133900.167398205576248No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC101350.12670506448956487No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT95560.11946656105202584No Hit
CGCTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG90200.11276563213575481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTGG8261800.044.2901271
GCTTGGG8220500.044.173822
CTTGGGG7680200.044.0887763
TTGGGGG3812050.043.782694
TGGGGGG1756400.043.5014765
TTGGGGA3040350.043.2206464
TGGGGGA912600.042.6757625
TGGGGAG1005700.042.5924225
TGGGGAC562150.042.432275
TGGGGGT670700.042.3169865
TTGGGGC545750.042.1354064
TGGGGGC550550.042.0813755
TTGGGGT412950.041.9118544
TGGGGAT1061950.041.0250935
TGGGGAA515900.040.997875
CTTGGGA317600.040.532433
TTGGGTA74200.040.2964974
TGGGGTA116400.039.2817885
TGGGGCA203700.039.089845
CTTGGGT187100.038.9561733