##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527829_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7998891
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31990747217333	33.0	31.0	34.0	31.0	34.0
2	32.689183163015976	34.0	31.0	34.0	31.0	34.0
3	32.95254879707699	34.0	31.0	34.0	31.0	34.0
4	36.38836471205821	37.0	37.0	37.0	35.0	37.0
5	36.43852891607099	37.0	37.0	37.0	35.0	37.0
6	36.1467829727896	37.0	37.0	37.0	35.0	37.0
7	36.55565952830211	37.0	37.0	37.0	35.0	37.0
8	36.62822921327469	37.0	37.0	37.0	35.0	37.0
9	38.55554538747934	39.0	39.0	39.0	37.0	39.0
10	37.89581630753563	39.0	38.0	39.0	35.0	39.0
11	36.81250650871477	39.0	35.0	39.0	33.0	39.0
12	36.55235019454572	39.0	35.0	39.0	33.0	39.0
13	36.349529703555156	39.0	35.0	39.0	33.0	39.0
14	37.204008280647905	40.0	35.0	40.0	33.0	40.0
15	37.31731411266887	40.0	35.0	40.0	33.0	40.0
16	37.40334441361934	40.0	35.0	40.0	33.0	40.0
17	37.2819653374449	40.0	35.0	40.0	33.0	40.0
18	37.30619832174235	40.0	35.0	40.0	33.0	40.0
19	37.27246051983956	40.0	35.0	40.0	33.0	40.0
20	37.24691022793035	40.0	35.0	40.0	33.0	40.0
21	37.286876768292004	40.0	35.0	40.0	33.0	40.0
22	37.19416679137145	40.0	35.0	40.0	33.0	40.0
23	37.224616887516035	40.0	35.0	40.0	33.0	40.0
24	37.258585596428304	40.0	35.0	40.0	33.0	40.0
25	37.20984471472358	39.0	35.0	40.0	33.0	40.0
26	37.04693088079335	39.0	35.0	40.0	33.0	40.0
27	36.97871492435639	39.0	35.0	40.0	33.0	40.0
28	36.885782416587496	39.0	35.0	40.0	33.0	40.0
29	36.839238964501455	39.0	35.0	40.0	32.0	40.0
30	36.67380265589318	39.0	35.0	40.0	32.0	40.0
31	36.58852370909917	39.0	35.0	40.0	31.0	40.0
32	36.54021076171684	38.0	35.0	40.0	32.0	40.0
33	36.28420427281732	38.0	35.0	40.0	31.0	40.0
34	36.04009055755354	38.0	35.0	40.0	30.0	40.0
35	36.070857322596346	38.0	35.0	40.0	30.0	40.0
36	36.04712090713575	38.0	35.0	40.0	30.0	40.0
37	36.13527637768786	38.0	35.0	40.0	31.0	40.0
38	35.81195318200986	38.0	35.0	40.0	30.0	40.0
39	35.88670729479874	38.0	35.0	40.0	30.0	40.0
40	35.78769431912499	38.0	34.0	40.0	30.0	40.0
41	35.47439751335529	37.0	34.0	40.0	29.0	40.0
42	35.56378065409317	37.0	34.0	40.0	30.0	40.0
43	35.588454699532726	37.0	34.0	40.0	30.0	40.0
44	35.48556731176859	37.0	34.0	40.0	30.0	40.0
45	35.363328991481445	37.0	34.0	40.0	29.0	40.0
46	35.02403970750445	36.0	34.0	40.0	28.0	40.0
47	34.76913262100959	36.0	34.0	40.0	27.0	40.0
48	34.789042506017395	36.0	34.0	40.0	27.0	40.0
49	34.81106380872048	35.0	34.0	40.0	28.0	40.0
50	34.687623446800316	35.0	34.0	39.0	28.0	40.0
51	35.01720501004452	35.0	34.0	40.0	29.0	40.0
52	34.78629274983245	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	16.0
15	100.0
16	316.0
17	911.0
18	1894.0
19	3499.0
20	6057.0
21	9493.0
22	14520.0
23	21187.0
24	29904.0
25	41167.0
26	53748.0
27	69562.0
28	89858.0
29	114820.0
30	148329.0
31	195329.0
32	265428.0
33	413650.0
34	902186.0
35	674392.0
36	669138.0
37	1093818.0
38	2190006.0
39	989553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.46060022570629	0.44036104504987006	0.07321014875687142	0.025828580486970006
8	99.22766793546755	0.6057339698715735	0.07752324666006825	0.08907484800080412
9	93.16018933124604	3.525888776331619	2.035057109791845	1.2788647826304922
10	48.47314458966874	38.10730762551959	6.1959964200037225	7.223551364807947
11	38.180755307204464	21.278774770152513	24.45043944216767	16.090030480475356
12	34.29155366662703	16.660046999015236	24.55781682735769	24.490582507000035
13	27.03060961825833	17.03139847761396	27.461869401645806	28.476122502481903
14	25.627502612549662	18.991870248013132	29.590439474672177	25.79018766476503
15	28.609265959493634	20.321179523511447	26.885151954189652	24.184402562805268
16	34.40928248678473	21.020288937553968	23.38541680340437	21.185011772256928
17	31.201937868637042	21.148094154552176	24.776797183509565	22.87317079330122
18	30.65192412298155	20.73496188409118	24.721539523416432	23.891574469510836
19	28.995894555882813	24.98918912634264	23.136132246332647	22.878784071441906
20	30.93653857766033	23.84275520193987	23.351324577369535	21.869381643030263
21	29.656523635588982	24.051559147386804	24.594821957193815	21.697095259830395
22	27.21613533676106	21.439722081473544	27.708391075712868	23.63575150605253
23	28.41412640827335	21.682755772018897	26.904092079764556	22.9990257399432
24	30.153892583359372	21.85888018726596	24.036657081587933	23.95057014778674
25	27.919557848706777	23.01984112547602	22.74329028861626	26.317310737200945
26	25.149286319816085	23.529874328828836	25.869823704311013	25.451015647044073
27	24.73690415333826	21.00634950520016	25.16252565511894	29.09422068634264
28	25.324135558291765	24.852870229135515	23.508433856643375	26.314560355929338
29	29.420703445015068	23.965784756911926	22.31418080331386	24.29933099475915
30	28.207072705453797	21.486741099484917	25.9822892948535	24.323896900207792
31	30.701080937344937	21.80320996998209	24.34645002663494	23.14925906603803
32	32.54224866922177	21.985697767353	23.216843434921167	22.255210128504064
33	32.52020811384978	21.808023137207392	23.45341372947825	22.21835501946457
34	27.424364202487574	22.96222063783592	28.192845733239768	21.42056942643674
35	25.32127266142269	27.67286115037697	26.000016752322292	21.005849435878048
36	25.986264845964275	29.078280976700395	23.245947469467957	21.68950670786738
37	29.049389471615505	26.696788342283952	21.608232941291487	22.64558924480906
38	28.095719769153	27.78172624180027	21.89819813771684	22.22435585132989
39	29.875916548931595	24.697536195955163	22.067221568589947	23.359325686523295
40	27.486222777632552	23.760718829647757	22.00425033920327	26.74880805351642
41	24.526224947933407	26.69126257627464	22.482104081678322	26.300408394113635
42	23.45213855270687	24.105954187899297	23.353287349458817	29.088619909935016
43	23.732077359223922	22.761930372597902	24.31805859087216	29.187933677306017
44	24.896638796553173	22.510958081564056	23.8119259282318	28.78047719365097
45	27.548168864908902	23.558903352977307	22.88099687819224	26.011930903921552
46	26.047385818859137	26.547942708557976	22.31226803815679	25.092403434426096
47	24.263588539961354	24.927380558129872	24.516748634279427	26.292282267629353
48	25.252475624433433	23.307893556744304	26.70017631194124	24.739454506881017
49	28.513852732835087	22.686132365099112	24.569718477223905	24.230296424841892
50	24.839705904230975	23.45535149810142	24.42359822130343	27.281344376364174
51	23.51120924138109	23.60480971674698	26.570070776061332	26.3139102658106
52	23.58114393607814	23.658617175805997	25.65350621729937	27.10673267081649
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	5.0
8	10.0
9	86.5
10	163.0
11	399.0
12	635.0
13	3010.0
14	11495.0
15	17605.0
16	21180.5
17	24756.0
18	21244.5
19	17733.0
20	17588.5
21	17444.0
22	18929.0
23	20414.0
24	20355.0
25	20296.0
26	22775.5
27	25255.0
28	30627.0
29	35999.0
30	43256.5
31	50514.0
32	59314.0
33	68114.0
34	78580.0
35	89046.0
36	100562.5
37	112079.0
38	123820.0
39	152518.0
40	169475.0
41	185412.0
42	201349.0
43	208879.0
44	216409.0
45	238955.0
46	261501.0
47	274581.0
48	287661.0
49	309294.0
50	330927.0
51	350922.5
52	370918.0
53	395442.0
54	419966.0
55	439972.0
56	459978.0
57	495768.0
58	531558.0
59	570084.5
60	608611.0
61	635394.5
62	662178.0
63	689164.5
64	739010.0
65	761869.0
66	625044.0
67	488219.0
68	402318.0
69	316417.0
70	260084.0
71	203751.0
72	169376.5
73	135002.0
74	113642.5
75	92283.0
76	79338.0
77	66393.0
78	51423.5
79	36454.0
80	24259.0
81	12064.0
82	8542.0
83	5020.0
84	3947.5
85	2875.0
86	1769.0
87	663.0
88	384.0
89	71.5
90	38.0
91	25.5
92	13.0
93	15.5
94	18.0
95	17.0
96	16.0
97	8.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	7998891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.0656220194135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.22964291574928	5.58380132297401
2	9.558676346920834	1.541933408403433
3	3.982725230504313	0.9636946894928787
4	1.9681770768207998	0.6349828947564294
5	1.0991612201922116	0.44327094702338543
6	0.6632716148615649	0.32098188850076326
7	0.4385830154271769	0.24762113785991458
8	0.3460234013120807	0.22327151718840574
9	0.25997349542249143	0.1887163154229187
>10	5.366723566194789	12.788605952921495
>50	4.366467073347221	25.065558704472444
>100	2.5964272170208647	36.77615732615927
>500	0.0849687070409541	4.559508094049401
>1k	0.034987114663535115	5.278215551261447
>5k	0.002740926033548834	1.5168320430140863
>10k+	0.0014510784883493828	3.866848206499641
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	117320	1.4667033217479772	No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	55684	0.6961465033090212	No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	30028	0.3754020401078099	No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	20846	0.2606111272175105	No Hit
CGCTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	20313	0.25394770350039775	No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	15483	0.19356433285564212	No Hit
CGCTTGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	14153	0.176937027895492	No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	13390	0.167398205576248	No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	10135	0.12670506448956487	No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	9556	0.11946656105202584	No Hit
CGCTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	9020	0.11276563213575481	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0011626611739052326	0.0	0.0	0.0	0.0
8	0.00420058230572213	0.0	0.0	0.0	0.0
9	0.012939293709590492	0.0	0.0	0.0	0.0
10	0.03718015409886195	0.0	0.0	0.0	0.0
11	0.04755659253263984	0.0	0.0	0.0	0.0
12	0.05658284379672132	0.0	0.0	0.0	0.0
13	0.06240865139930023	0.0	0.0	0.0	0.0
14	0.07263506903644518	0.0	0.0	0.0	0.0
15	0.08514930382224235	0.0	0.0	0.0	0.0
16	0.09681342076045292	0.0	0.0	0.0	0.0
17	0.10810248570708114	0.0	0.0	0.0	0.0
18	0.11871645706886116	0.0	0.0	0.0	0.0
19	0.13353101073636334	0.0	0.0	0.0	0.0
20	0.1461827645857407	0.0	0.0	0.0	0.0
21	0.15665921688394055	0.0	0.0	0.0	0.0
22	0.16642307039813395	0.0	0.0	0.0	0.0
23	0.17787465787444784	0.0	0.0	0.0	0.0
24	0.18850113096928062	0.0	0.0	0.0	0.0
25	0.19915260753021888	0.0	0.0	0.0	0.0
26	0.20974157542589342	0.0	0.0	0.0	0.0
27	0.2198054705333527	0.0	0.0	0.0	0.0
28	0.2319071481284093	0.0	0.0	0.0	0.0
29	0.24244610909187286	0.0	0.0	0.0	0.0
30	0.25308508391975837	0.0	0.0	0.0	0.0
31	0.26317398249332313	0.0	0.0	0.0	0.0
32	0.27381295732120864	0.0	0.0	0.0	0.0
33	0.2840893818905646	0.0	0.0	0.0	0.0
34	0.29501589657866323	0.0	0.0	0.0	0.0
35	0.3088303116019458	0.0	0.0	0.0	0.0
36	0.32249470582859546	0.0	0.0	0.0	0.0
37	0.3351339579449201	0.0	0.0	0.0	0.0
38	0.346535538489023	0.0	0.0	0.0	0.0
39	0.35687447172364267	0.0	0.0	0.0	0.0
40	0.368501083462695	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTGG	826180	0.0	44.290127	1
GCTTGGG	822050	0.0	44.17382	2
CTTGGGG	768020	0.0	44.088776	3
TTGGGGG	381205	0.0	43.78269	4
TGGGGGG	175640	0.0	43.501476	5
TTGGGGA	304035	0.0	43.220646	4
TGGGGGA	91260	0.0	42.675762	5
TGGGGAG	100570	0.0	42.592422	5
TGGGGAC	56215	0.0	42.43227	5
TGGGGGT	67070	0.0	42.316986	5
TTGGGGC	54575	0.0	42.135406	4
TGGGGGC	55055	0.0	42.081375	5
TTGGGGT	41295	0.0	41.911854	4
TGGGGAT	106195	0.0	41.025093	5
TGGGGAA	51590	0.0	40.99787	5
CTTGGGA	31760	0.0	40.53243	3
TTGGGTA	7420	0.0	40.296497	4
TGGGGTA	11640	0.0	39.281788	5
TGGGGCA	20370	0.0	39.08984	5
CTTGGGT	18710	0.0	38.956173	3
>>END_MODULE