FastQCFastQC Report
Fri 17 Jun 2016
SRR1527828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862475
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC524181.8312124996724861No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG363831.2710329347854565No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT194020.6778050463322823No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT171500.5991318701473376No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT120380.42054515759962974No Hit
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC98940.34564494013048147No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG83890.29306806172979677No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA76290.2665176115075241No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC74920.2617315435069302No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC62790.21935562756006602No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC55230.1929449165494895No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC49620.17334649210910139No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG46890.16380929091083765No Hit
GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG45800.16000139739211697No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG44510.1554948078149154No Hit
GACACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT43860.15322404562485262No Hit
GACACTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTG41770.14592267181372764No Hit
GACACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC36140.1262543777674914No Hit
GACACTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC35550.12419322439497288No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC33260.11619315452536703No Hit
GACACTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG32250.1126647394300387No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG31040.10843762827622949No Hit
GACACTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC30210.10553803963353392No Hit
GACACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC30000.10480440877212903No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTCAATGGC29040.10145066769142089No Hit
GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG28830.10071703683001598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG254.4445074E-544.029
TCGTCTA207.8584504E-444.028
TACCGAC351.447097E-744.040
ACACTGG2994650.041.6285742
ACTGGGC289850.041.6091084
CACTGGG2960350.041.5572473
ACTGGGA707300.041.5023354
GACACTG3019100.041.502041
ACTGGGG1819300.041.398894
CTGGGGG831800.040.9584055
TGGGGGG452600.040.5731356
CTGGGAT211850.040.4899675
CTGGGGC313800.040.3753975
ACTGGGT156000.040.3333364
CTGGGGA448500.040.2622075
CTGGGGT262300.040.1082735
CACTAGG12100.039.818183
TGGGATT69400.039.815566
CTGGGAG257450.039.59065
CTGGGAC127600.039.586215
Produced by FastQC (version 0.11.5)