FastQCFastQC Report
Fri 17 Jun 2016
SRR1527827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4279473
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC777641.81713963378201No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT475421.1109311824142831No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC292080.6825139450581883No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC231550.541071295460913No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG186520.4358480588614533No Hit
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT143870.33618625470939995No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC125340.2928865306545923No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC112830.26365395926087165No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC112410.26267253000544694No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC93460.21839137669521458No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA82750.19336493068188537No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC74240.1734793045779235No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT72940.17044154735875186No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC70270.16420246137783787No Hit
GACACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT67310.15728572186341636No Hit
GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT67020.15660806832990884No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG65510.1530795964830249No Hit
GACACTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT63450.1482659196587991No Hit
GACACTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT55560.12982907007474986No Hit
GACACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT54700.1278194768374517No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT46200.10795721809671426No Hit
GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG45530.10639160476067963No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTCAATGGCTG44510.10400813371179114No Hit
GACACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA44300.10351741908407881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG253.4187276E-546.011
ACTGGGA1067250.043.5755464
ACACTGG4476150.043.5443842
CACTGGG4423950.043.448343
GACACTG4515650.043.395751
ACTGGGG2716950.043.2462164
ACTGGGC438550.043.2203834
CTGGGGG1243150.042.819615
TGGGGGG677050.042.273396
CTGGGAT318000.042.2172975
CACTAGG16400.042.2134133
CTGGGGC475600.042.107025
CTGGGGA672850.042.0587045
ACTGGGT221400.041.8030744
TGGGATT107150.041.6210946
CTGGGAC189600.041.584395
CTGGGGT384750.041.432885
TGGGGCT266600.041.2982036
CTGGGAG394300.041.193515
TGGGGGT181550.040.9451946
Produced by FastQC (version 0.11.5)