##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4279473
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35502525661454	33.0	31.0	34.0	31.0	34.0
2	32.466827340656195	34.0	31.0	34.0	31.0	34.0
3	32.868683597256016	34.0	31.0	34.0	31.0	34.0
4	36.341993278144294	37.0	37.0	37.0	35.0	37.0
5	36.34988209996885	37.0	37.0	37.0	35.0	37.0
6	36.317593544812645	37.0	37.0	37.0	35.0	37.0
7	36.44092321648016	37.0	37.0	37.0	35.0	37.0
8	36.61005665884561	37.0	37.0	37.0	35.0	37.0
9	38.70457554002561	39.0	39.0	39.0	39.0	39.0
10	38.10752468820343	39.0	38.0	39.0	37.0	39.0
11	37.78032902649462	39.0	38.0	39.0	35.0	39.0
12	37.07898028565667	39.0	35.0	39.0	34.0	39.0
13	36.932791023567624	39.0	35.0	39.0	33.0	39.0
14	37.65687317106569	40.0	36.0	40.0	33.0	40.0
15	37.70935907295127	40.0	36.0	40.0	33.0	40.0
16	37.736136201817374	40.0	36.0	40.0	33.0	40.0
17	37.54940900433301	40.0	36.0	40.0	33.0	40.0
18	37.55321040698235	40.0	36.0	40.0	33.0	40.0
19	37.539154003308354	40.0	36.0	40.0	33.0	40.0
20	37.53731195406537	40.0	36.0	40.0	33.0	40.0
21	37.52555022546	40.0	36.0	40.0	33.0	40.0
22	37.529126133054234	40.0	36.0	40.0	33.0	40.0
23	37.52561284999344	40.0	36.0	40.0	33.0	40.0
24	37.49716916078218	40.0	35.0	40.0	33.0	40.0
25	37.46355053531124	40.0	35.0	40.0	33.0	40.0
26	37.31926781638767	40.0	35.0	40.0	33.0	40.0
27	37.24404126395937	40.0	35.0	40.0	33.0	40.0
28	37.090760708152615	39.0	35.0	40.0	33.0	40.0
29	37.01092517700194	39.0	35.0	40.0	32.0	40.0
30	36.841827486702215	39.0	35.0	40.0	32.0	40.0
31	36.7437957898087	39.0	35.0	40.0	31.0	40.0
32	36.655611333451574	39.0	35.0	40.0	31.0	40.0
33	36.54538771479572	39.0	35.0	40.0	31.0	40.0
34	36.11007149712126	39.0	35.0	40.0	30.0	40.0
35	36.00901466138471	38.0	35.0	40.0	30.0	40.0
36	36.12933041054354	38.0	35.0	40.0	30.0	40.0
37	36.11515156188624	38.0	35.0	40.0	30.0	40.0
38	36.05147713281519	38.0	35.0	40.0	30.0	40.0
39	35.943974643606815	38.0	35.0	40.0	30.0	40.0
40	35.71243935877151	38.0	34.0	40.0	30.0	40.0
41	35.59423672026906	38.0	34.0	40.0	30.0	40.0
42	35.492290289014555	37.0	34.0	40.0	29.0	40.0
43	35.3492708097469	37.0	34.0	40.0	29.0	40.0
44	35.24483318389905	37.0	34.0	40.0	29.0	40.0
45	35.06499258203054	36.0	34.0	40.0	28.0	40.0
46	34.87042867194162	36.0	34.0	40.0	27.0	40.0
47	34.70687769265047	36.0	33.0	40.0	27.0	40.0
48	34.53152011941657	35.0	33.0	40.0	26.0	40.0
49	34.86194865582748	35.0	34.0	40.0	28.0	40.0
50	34.92826149388021	35.0	34.0	40.0	29.0	40.0
51	34.93535909678598	35.0	34.0	40.0	29.0	40.0
52	34.48440847739897	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	7.0
15	30.0
16	120.0
17	306.0
18	684.0
19	1303.0
20	2417.0
21	4236.0
22	7138.0
23	10939.0
24	15928.0
25	22147.0
26	29673.0
27	38802.0
28	49844.0
29	63207.0
30	79348.0
31	99332.0
32	127953.0
33	180334.0
34	396835.0
35	382544.0
36	396365.0
37	619361.0
38	1190016.0
39	560602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.55024836002003	0.3663768879953209	0.07040586539510824	0.012968886589540348
8	99.45554043687154	0.4323429543777937	0.07391096987876779	0.03820563887188913
9	98.54038102355126	0.7305338764843241	0.2793801947108908	0.44970490525352075
10	60.72313109581483	24.07420259457181	5.186947084372305	10.015719225241051
11	40.85147867506116	23.063610869843085	18.96446127829291	17.120449176802843
12	36.6120314347117	15.3066277085987	24.277895899798878	23.803444956890722
13	27.631930380212705	15.171447512345562	26.02717671077724	31.169445396664496
14	24.840254863157217	17.159869918562404	30.931098291775644	27.068776926504732
15	29.48068605643732	16.953956713829015	27.37545020146172	26.189907028271943
16	33.96329407849985	18.49445013439739	23.672097008206382	23.870158778896375
17	31.450928654065578	18.607548172403472	23.51130618185931	26.43021699167164
18	32.42595525196677	20.256676464602066	22.615448210562374	24.701920072868784
19	30.87777396889757	22.381073557421676	21.511013155124477	25.230139318556283
20	33.427223398768966	21.250023075271184	21.779457423846345	23.54329610211351
21	33.59780515030706	22.41364766175648	22.158335851166722	21.830211336769736
22	30.97876771275342	19.442604264590525	25.456615802927136	24.122012219728926
23	31.855557915659244	20.174727121774104	24.276774266364107	23.692940696202548
24	33.67031407839236	19.753647236470474	22.028646985271315	24.547391699865848
25	29.594064502802098	19.694200664427605	21.59469168283104	29.11704314993926
26	27.20622375699064	19.19972389123614	24.44728591581253	29.146766435960693
27	25.216749819428703	17.862760204352266	23.977414976096355	32.943075000122676
28	27.32049016315794	23.17152135321335	21.440373616097123	28.06761486753159
29	31.932530010120402	21.616867310530992	20.961973588804042	25.488629090544563
30	30.13312620502571	19.537218718286105	24.378211990121212	25.951443086566968
31	34.2064314928497	19.323687753141567	21.776746809712318	24.69313394429641
32	36.10295005950499	19.49462001512803	21.131550543723492	23.270879381643486
33	36.443389174321226	19.122751796774974	21.64060855156698	22.793250477336812
34	31.164421413571255	21.031491494396622	26.478540698819693	21.325546393212434
35	28.16323411784582	25.30725161719679	25.77417826914669	20.7553359958107
36	27.90584261192909	27.743743213241444	21.93896304521608	22.41145112961339
37	31.38631789475013	26.09101634710629	19.79668991952981	22.725975838613774
38	31.332806632966253	26.020330073352486	20.332877436076828	22.31398585760443
39	32.64862285613205	23.043398100653985	19.697004748014535	24.61097429519943
40	29.382473028805183	21.924218239021485	20.035691310588945	28.657617421584387
41	26.88057618309544	24.385385770631103	20.759775794823334	27.97426225145012
42	25.07112441181426	22.302442380171577	21.179336801517383	31.447096406496783
43	24.938351053973236	20.372999198733115	23.138433166887605	31.550216580406044
44	25.91323744769508	19.085106974620473	22.94579262446568	32.05586295321877
45	30.17415929484775	19.67954932768591	22.222432528491243	27.923858848975097
46	27.287542181011542	24.255883843641495	21.657292848909197	26.799281126437762
47	26.141139341222626	22.158312483803495	23.01872216508902	28.681826009884865
48	27.597650458362516	20.499907348404815	26.252064214448833	25.650377978783833
49	30.12634966969064	19.502868694346244	24.64263707236849	25.72814456359463
50	26.67835502175151	20.41008320417023	24.032188075494343	28.879373698583915
51	24.999246402536013	20.830952783204847	26.528196345671535	27.641604468587605
52	24.77438226622764	20.54005247842433	25.088790138411905	29.596775116936126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	48.5
6	97.0
7	65.5
8	34.0
9	158.0
10	282.0
11	894.0
12	1506.0
13	4437.5
14	8758.0
15	10147.0
16	9214.5
17	8282.0
18	7449.0
19	6616.0
20	7153.5
21	7691.0
22	7884.5
23	8078.0
24	8494.0
25	8910.0
26	10456.5
27	12003.0
28	14991.0
29	17979.0
30	19339.5
31	20700.0
32	22136.5
33	23573.0
34	29693.5
35	35814.0
36	39090.5
37	42367.0
38	47405.5
39	60506.0
40	68568.0
41	72404.0
42	76240.0
43	82132.5
44	88025.0
45	93145.5
46	98266.0
47	111710.5
48	125155.0
49	134331.0
50	143507.0
51	155789.5
52	168072.0
53	187748.5
54	207425.0
55	226115.5
56	244806.0
57	279166.5
58	313527.0
59	355941.5
60	398356.0
61	429188.5
62	460021.0
63	488251.5
64	424841.0
65	333200.0
66	295524.5
67	257849.0
68	210240.5
69	162632.0
70	142002.0
71	121372.0
72	102369.5
73	83367.0
74	80589.5
75	77812.0
76	61050.0
77	44288.0
78	30365.0
79	16442.0
80	11194.0
81	5946.0
82	4526.5
83	3107.0
84	1889.5
85	672.0
86	459.0
87	246.0
88	164.0
89	58.5
90	35.0
91	33.5
92	32.0
93	40.5
94	49.0
95	24.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4279473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.541456743813016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.00609872653658	5.1286505186165305
2	9.896948270356974	1.4927481455330431
3	4.248973937440118	0.9613035946438055
4	2.222846866130007	0.6705401395615906
5	1.2550368078833771	0.47324028992728273
6	0.7553077384918897	0.3417672382862291
7	0.5462235952937581	0.2883525131450532
8	0.39711565661944787	0.23958644373491753
9	0.3295307527703503	0.22366277259963643
>10	5.292975191301197	11.224653311173556
>50	4.180938251434868	22.66874386728847
>100	2.702429343691396	35.3607442630386
>500	0.1021302887411046	5.145944103169994
>1k	0.05725485883971014	8.25330265777649
>5k	0.003404342958036819	1.7906475759221023
>10k+	0.002785371511121034	5.736112565582714
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	77764	1.81713963378201	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	47542	1.1109311824142831	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	29208	0.6825139450581883	No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	23155	0.541071295460913	No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	18652	0.4358480588614533	No Hit
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	14387	0.33618625470939995	No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	12534	0.2928865306545923	No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	11283	0.26365395926087165	No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	11241	0.26267253000544694	No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	9346	0.21839137669521458	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8275	0.19336493068188537	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	7424	0.1734793045779235	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	7294	0.17044154735875186	No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	7027	0.16420246137783787	No Hit
GACACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6731	0.15728572186341636	No Hit
GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6702	0.15660806832990884	No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	6551	0.1530795964830249	No Hit
GACACTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT	6345	0.1482659196587991	No Hit
GACACTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	5556	0.12982907007474986	No Hit
GACACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	5470	0.1278194768374517	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	4620	0.10795721809671426	No Hit
GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4553	0.10639160476067963	No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTCAATGGCTG	4451	0.10400813371179114	No Hit
GACACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	4430	0.10351741908407881	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.009089904294290442	0.0	0.0	0.0	0.0
8	0.011683681612198512	0.0	0.0	0.0	0.0
9	0.014487765199126154	0.0	0.0	0.0	0.0
10	0.03135900144714081	0.0	0.0	0.0	0.0
11	0.04505227629663746	0.0	0.0	0.0	0.0
12	0.05271677143423968	0.0	0.0	0.0	0.0
13	0.05771738716426065	0.0	0.0	0.0	0.0
14	0.06636331155728754	0.0	0.0	0.0	0.0
15	0.07521954221933401	0.0	0.0	0.0	0.0
16	0.08821179617209876	0.0	0.0	0.0	0.0
17	0.09996557987397046	0.0	0.0	0.0	0.0
18	0.11050426068817353	0.0	0.0	0.0	0.0
19	0.12653427186010988	0.0	0.0	0.0	0.0026405120443568635
20	0.13856846392067435	0.0	0.0	0.0	0.0026405120443568635
21	0.14873326692328706	0.0	0.0	0.0	0.0026872467708056575
22	0.15728572186341636	0.0	0.0	0.0	0.0026872467708056575
23	0.1688058319330441	0.0	0.0	0.0	0.0026872467708056575
24	0.18226543315029678	0.0	0.0	0.0	0.0026872467708056575
25	0.19238350142646068	0.0	0.0	0.0	0.0026872467708056575
26	0.20864718623064102	0.0	0.0	0.0	0.0026872467708056575
27	0.22098515401312263	0.0	0.0	0.0	0.0026872467708056575
28	0.24383843524658294	0.0	0.0	0.0	0.0026872467708056575
29	0.25519497377363987	0.0	0.0	0.0	0.0026872467708056575
30	0.26783671727803865	0.0	0.0	0.0	0.0026872467708056575
31	0.27737060147359266	0.0	0.0	0.0	0.0026872467708056575
32	0.2897786713457475	0.0	0.0	0.0	0.0026872467708056575
33	0.2996630659896674	0.0	0.0	0.0	0.0026872467708056575
34	0.31214123795149545	0.0	0.0	0.0	0.0026872467708056575
35	0.33396635520308227	0.0	0.0	0.0	0.0026872467708056575
36	0.35037024418660895	0.0	0.0	0.0	0.0026872467708056575
37	0.3649982135650815	0.0	0.0	0.0	0.0026872467708056575
38	0.37633138472891403	0.0	0.0	0.0	0.0026872467708056575
39	0.38743088226050265	0.0	0.0	0.0	0.0026872467708056575
40	0.3994183395946183	0.0	0.0	0.0	0.0026872467708056575
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACG	25	3.4187276E-5	46.0	11
ACTGGGA	106725	0.0	43.575546	4
ACACTGG	447615	0.0	43.544384	2
CACTGGG	442395	0.0	43.44834	3
GACACTG	451565	0.0	43.39575	1
ACTGGGG	271695	0.0	43.246216	4
ACTGGGC	43855	0.0	43.220383	4
CTGGGGG	124315	0.0	42.81961	5
TGGGGGG	67705	0.0	42.27339	6
CTGGGAT	31800	0.0	42.217297	5
CACTAGG	1640	0.0	42.213413	3
CTGGGGC	47560	0.0	42.10702	5
CTGGGGA	67285	0.0	42.058704	5
ACTGGGT	22140	0.0	41.803074	4
TGGGATT	10715	0.0	41.621094	6
CTGGGAC	18960	0.0	41.58439	5
CTGGGGT	38475	0.0	41.43288	5
TGGGGCT	26660	0.0	41.298203	6
CTGGGAG	39430	0.0	41.19351	5
TGGGGGT	18155	0.0	40.945194	6
>>END_MODULE