SRR1527826_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527826_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4301327
Sequences flagged as poor quality	0
Sequence length	52
%GC	58

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	74310	1.7276063875171548	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	45551	1.058998769449521	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	27691	0.6437780712789333	No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	22430	0.5214669798413374	No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	17843	0.4148254713022283	No Hit
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	14112	0.32808479801698404	No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	12000	0.27898367178314976	No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	10859	0.25245697432443526	No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	10772	0.2504343427040074	No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	8917	0.2073081167741955	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7941	0.18461744480249934	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	7089	0.1648096041058957	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	7031	0.16346118302561047	No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6787	0.15778851503268643	No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	6379	0.14830307019205932	No Hit
GACACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6348	0.1475823623732862	No Hit
GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6208	0.14432755286914947	No Hit
GACACTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT	5860	0.1362370263874381	No Hit
GACACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	5150	0.11973049247360175	No Hit
GACACTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	5147	0.11966074655565596	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	4588	0.10666475717842423	No Hit
GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4314	0.10029463000604232	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACACTGG	450195	43.499702	2
ACTGGGA	105400	43.473053	4
CACTGGG	444625	43.410446	3
ACTGGGC	43880	43.384457	4
GACACTG	454000	43.36564	1
ACTGGGG	273945	43.19327	4
CTGGGGG	125330	42.74443	5
CTGGGAT	32040	42.48252	5
TGGGGGG	68605	42.288754	6
CTGGGGC	47875	42.18068	5
CTGGGGA	67865	41.86871	5
TGGGATT	10625	41.692234	6
ACTGGGT	23590	41.671047	4
CACTAGG	1790	41.374302	3
CTGGGGT	39100	41.294117	5
ACACTAG	1910	41.183247	2
TGGGGCT	26995	41.126507	6
CTGGGAG	37695	41.033295	5
CTGGGAC	19460	40.846867	5
TGGGGAG	29440	40.734375	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content