##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527826_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4301327
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.305488283034514	33.0	31.0	34.0	31.0	34.0
2	32.437576589736146	34.0	31.0	34.0	31.0	34.0
3	32.85634526275263	34.0	31.0	34.0	31.0	34.0
4	36.32394909756919	37.0	37.0	37.0	35.0	37.0
5	36.35387149128629	37.0	37.0	37.0	35.0	37.0
6	36.26196520283159	37.0	37.0	37.0	35.0	37.0
7	36.396325366567105	37.0	36.0	37.0	35.0	37.0
8	36.58110973659989	37.0	37.0	37.0	35.0	37.0
9	38.660358070893004	39.0	39.0	39.0	38.0	39.0
10	38.013724834219765	39.0	38.0	39.0	37.0	39.0
11	37.73891406070731	39.0	38.0	39.0	35.0	39.0
12	37.028167353935196	39.0	35.0	39.0	34.0	39.0
13	36.730232553814204	39.0	35.0	39.0	33.0	39.0
14	37.59106061919961	40.0	36.0	40.0	33.0	40.0
15	37.66386466316093	40.0	36.0	40.0	33.0	40.0
16	37.719090178449584	40.0	36.0	40.0	33.0	40.0
17	37.56747882688296	40.0	36.0	40.0	33.0	40.0
18	37.584430804726075	40.0	36.0	40.0	33.0	40.0
19	37.52291746244822	40.0	36.0	40.0	33.0	40.0
20	37.53112237223536	40.0	36.0	40.0	33.0	40.0
21	37.580751010095256	40.0	36.0	40.0	33.0	40.0
22	37.47064987153964	40.0	36.0	40.0	33.0	40.0
23	37.490670437285985	40.0	35.0	40.0	33.0	40.0
24	37.50866209427928	40.0	35.0	40.0	33.0	40.0
25	37.44683257050673	40.0	35.0	40.0	33.0	40.0
26	37.25719899928557	40.0	35.0	40.0	33.0	40.0
27	37.1798537986068	40.0	35.0	40.0	33.0	40.0
28	37.04764692384467	39.0	35.0	40.0	33.0	40.0
29	36.969822801196	39.0	35.0	40.0	32.0	40.0
30	36.78520721628465	39.0	35.0	40.0	32.0	40.0
31	36.70585170576429	39.0	35.0	40.0	31.0	40.0
32	36.62357663111872	39.0	35.0	40.0	31.0	40.0
33	36.34498958112229	39.0	35.0	40.0	31.0	40.0
34	36.02467215350054	38.0	35.0	40.0	29.0	40.0
35	35.99878502610938	38.0	35.0	40.0	30.0	40.0
36	35.974243297475404	38.0	35.0	40.0	30.0	40.0
37	36.02898338117516	38.0	35.0	40.0	30.0	40.0
38	35.698963598907966	38.0	34.0	40.0	30.0	40.0
39	35.76509319100826	38.0	34.0	40.0	30.0	40.0
40	35.613011519468294	38.0	34.0	40.0	30.0	40.0
41	35.299166513031906	37.0	34.0	40.0	28.0	40.0
42	35.335075198886294	37.0	34.0	40.0	29.0	40.0
43	35.30613947742174	37.0	34.0	40.0	29.0	40.0
44	35.20521806409975	37.0	34.0	40.0	29.0	40.0
45	35.0261131041653	36.0	34.0	40.0	28.0	40.0
46	34.65781722710224	36.0	33.0	40.0	26.0	40.0
47	34.41758554976174	36.0	33.0	40.0	26.0	40.0
48	34.40281220190885	35.0	33.0	39.0	26.0	40.0
49	34.33846810530797	35.0	33.0	39.0	26.0	40.0
50	34.173869598847055	35.0	33.0	39.0	26.0	40.0
51	34.51703648664703	35.0	34.0	39.0	27.0	40.0
52	34.275717935418534	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	8.0
15	49.0
16	115.0
17	335.0
18	774.0
19	1426.0
20	2628.0
21	4739.0
22	7633.0
23	11567.0
24	16757.0
25	23342.0
26	31231.0
27	40337.0
28	51744.0
29	65804.0
30	82765.0
31	103497.0
32	134584.0
33	195643.0
34	405836.0
35	383976.0
36	420714.0
37	653247.0
38	1177653.0
39	484918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.53279534432049	0.3777438915943847	0.07437239716952465	0.015088366915605347
8	99.45096013393076	0.4354237657355509	0.07516285090624358	0.03845324942744414
9	98.51283104028128	0.733215586724748	0.2997679553309944	0.45418541766296777
10	60.693246526013944	24.09509902409187	5.2195287640302634	9.99212568586392
11	40.86743463121962	23.08276027374808	18.930274308370418	17.119530786661883
12	36.588731802999405	15.308159551691839	24.316751551323577	23.786357093985185
13	27.862331787376316	15.183198115372305	25.95457169380519	30.999898403446192
14	24.798277368821296	17.157449317385076	30.96397925570411	27.080294058089514
15	29.52319133141935	16.926799566738357	27.345049562611724	26.204959539230565
16	34.00092576081754	18.51117108743418	23.639611682627244	23.84829146912104
17	31.424767286932614	18.624275717702933	23.491959574336015	26.458997421028442
18	32.38112331380525	20.255516495258323	22.60374530929641	24.759614881640015
19	30.894907548298466	22.38755621230378	21.474582146393427	25.24295409300432
20	33.41131701914316	21.29863644405552	21.7636557276394	23.526390809161917
21	33.630528439246774	22.399203780600732	22.12940332134711	21.840864458805388
22	31.058089747652296	19.418123755761883	25.44998322610673	24.07380327047909
23	31.83573348410851	20.15040939691402	24.30894000851365	23.704917110463818
24	33.710898985359634	19.732677845697385	22.007278219024037	24.549144949918944
25	29.636202967130842	19.65307450468193	21.589035197742465	29.121687330444768
26	27.216972808623947	19.20237638291625	24.439899593776527	29.14075121468328
27	25.254113439875645	17.807830002229544	24.02068012964371	32.917376428251096
28	27.36590359207752	23.118214448703853	21.44856691899965	28.067315040218983
29	31.93737653519484	21.622768973388908	20.93305159082302	25.506802900593232
30	30.147370799755517	19.544084883571976	24.387869138988968	25.92067517768354
31	34.20142202627236	19.29497571330894	21.824590411284703	24.679011849134
32	36.1565860954073	19.47715205098334	21.098581902747686	23.267679950861677
33	36.3562919071254	19.18656730818187	21.694723512069647	22.762417272623075
34	31.202952019225695	21.02530219162598	26.472156150880878	21.299589638267445
35	28.066571083760895	25.325835492163236	25.814010420505113	20.79358300357076
36	28.01963208098338	27.693616411865456	21.91116834409474	22.375583163056426
37	31.401146669388307	26.034918991278737	19.78173247465259	22.782201864680367
38	31.294202928538102	26.02180675870493	20.29092417293547	22.393066139821503
39	32.58566484250093	23.073949039447594	19.69947878875519	24.640907329296287
40	29.451027554984776	21.8483505206649	19.998712025381934	28.701909898968385
41	27.118677561599014	24.349997105544404	20.75127048001698	27.7800548528396
42	25.071518626693578	22.31181214541466	21.20289854735527	31.413770680536494
43	24.956414613443712	20.335445317224195	23.120492815356748	31.587647253975344
44	25.909446084894267	19.07915859454536	22.942803465070195	32.06859185549018
45	30.16487702515991	19.644681745889116	22.218282869449357	27.972158359501613
46	27.469894755734686	24.20125231120536	21.63053401892021	26.69831891413975
47	26.29879104750697	22.17759775064765	22.9598679663276	28.563743235517787
48	27.597181055985747	20.49632590128581	26.270869431689338	25.635623611039104
49	30.1945655375655	19.479686152668698	24.58943484185229	25.736313467913508
50	26.752279005990477	20.309871813977406	23.961558839865	28.976290340167115
51	24.98242984083749	20.759105271466225	26.58788787739226	27.670577010304033
52	24.807530327268772	20.48709619147765	25.106577574780992	29.598795906472585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	49.5
6	99.0
7	72.5
8	46.0
9	155.5
10	265.0
11	870.0
12	1475.0
13	4295.5
14	8615.0
15	10114.0
16	9163.0
17	8212.0
18	7378.5
19	6545.0
20	7069.0
21	7593.0
22	7888.5
23	8184.0
24	8572.0
25	8960.0
26	10556.5
27	12153.0
28	15033.5
29	17914.0
30	19302.0
31	20690.0
32	22239.0
33	23788.0
34	29596.0
35	35404.0
36	39177.5
37	42951.0
38	48013.5
39	60979.0
40	68882.0
41	73071.0
42	77260.0
43	82601.0
44	87942.0
45	93292.5
46	98643.0
47	111531.0
48	124419.0
49	134290.5
50	144162.0
51	156467.0
52	168772.0
53	188620.5
54	208469.0
55	227535.0
56	246601.0
57	281637.5
58	316674.0
59	357978.0
60	399282.0
61	430637.5
62	461993.0
63	489350.0
64	426910.0
65	337113.0
66	298072.0
67	259031.0
68	211738.5
69	164446.0
70	143492.5
71	122539.0
72	103345.5
73	84152.0
74	81208.0
75	78264.0
76	61176.5
77	44089.0
78	30466.0
79	16843.0
80	11510.0
81	6177.0
82	4626.5
83	3076.0
84	1908.5
85	741.0
86	514.0
87	287.0
88	178.0
89	52.5
90	36.0
91	34.5
92	33.0
93	36.5
94	40.0
95	20.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4301327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.998192887016746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04836881920119	5.44263979462827
2	9.968282451561663	1.594564915997087
3	4.342142633228837	1.0418788297050916
4	2.2354457273281074	0.7151810446251058
5	1.239495349149485	0.4956861442528874
6	0.7704204551667432	0.3697182842716109
7	0.5646465850335444	0.31613066100655135
8	0.429944050890366	0.27510203597171934
9	0.3117160870267203	0.22438488510232263
>10	5.475067021216182	12.361931548968457
>50	4.045544739492706	23.19282288312479
>100	2.417241735452365	33.64867821551185
>500	0.09343755667537858	5.006321015484451
>1k	0.05217941476676986	7.831331524001628
>5k	0.003337055595549235	1.767803196989006
>10k+	0.0027303182145402833	5.715825020359144
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	74310	1.7276063875171548	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	45551	1.058998769449521	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	27691	0.6437780712789333	No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	22430	0.5214669798413374	No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	17843	0.4148254713022283	No Hit
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	14112	0.32808479801698404	No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	12000	0.27898367178314976	No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	10859	0.25245697432443526	No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	10772	0.2504343427040074	No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	8917	0.2073081167741955	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7941	0.18461744480249934	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	7089	0.1648096041058957	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	7031	0.16346118302561047	No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6787	0.15778851503268643	No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	6379	0.14830307019205932	No Hit
GACACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6348	0.1475823623732862	No Hit
GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6208	0.14432755286914947	No Hit
GACACTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT	5860	0.1362370263874381	No Hit
GACACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	5150	0.11973049247360175	No Hit
GACACTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	5147	0.11966074655565596	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	4588	0.10666475717842423	No Hit
GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4314	0.10029463000604232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.009229709808159204	0.0	0.0	0.0	0.0
8	0.011903303329414387	0.0	0.0	0.0	0.0
9	0.01464664276861536	0.0	0.0	0.0	0.0
10	0.03182738722259433	0.0	0.0	0.0	0.0
11	0.04505586299297868	0.0	0.0	0.0	0.0
12	0.052472178934547406	0.0	0.0	0.0	0.0
13	0.05821459284541724	0.0	0.0	0.0	0.0
14	0.06681658939206436	0.0	0.0	0.0	0.0
15	0.07623228831474566	0.0	0.0	0.0	0.0
16	0.08762412157922427	0.0	0.0	0.0	0.0
17	0.09855098205739764	0.0	0.0	0.0	0.0
18	0.10943134525694047	0.0	0.0	0.0	0.0
19	0.12489169040159002	0.0	0.0	0.0	0.002231869374265198
20	0.13714372332073335	0.0	0.0	0.0	0.002231869374265198
21	0.1457689685066957	0.0	0.0	0.0	0.0022783666528957227
22	0.154649948725126	0.0	0.0	0.0	0.0022783666528957227
23	0.16590229015371302	0.0	0.0	0.0	0.0022783666528957227
24	0.17859604721984634	0.0	0.0	0.0	0.0022783666528957227
25	0.18845347028951764	0.0	0.0	0.0	0.0022783666528957227
26	0.20405330727005874	0.0	0.0	0.0	0.0022783666528957227
27	0.21472443271576422	0.0	0.0	0.0	0.0022783666528957227
28	0.23732211013019935	0.0	0.0	0.0	0.0022783666528957227
29	0.24871394339467798	0.0	0.0	0.0	0.0022783666528957227
30	0.26098922495313653	0.0	0.0	0.0	0.0022783666528957227
31	0.27070715618691626	0.0	0.0	0.0	0.0022783666528957227
32	0.2836333996462022	0.0	0.0	0.0	0.0022783666528957227
33	0.2949787356320503	0.0	0.0	0.0	0.0022783666528957227
34	0.3069285362400952	0.0	0.0	0.0	0.0022783666528957227
35	0.3283870303280825	0.0	0.0	0.0	0.0022783666528957227
36	0.3446610778487662	0.0	0.0	0.0	0.0022783666528957227
37	0.3588659964703916	0.0	0.0	0.0	0.0022783666528957227
38	0.3708157970784365	0.0	0.0	0.0	0.0022783666528957227
39	0.3808592092626299	0.0	0.0	0.0	0.0022783666528957227
40	0.3912048537579217	0.0	0.0	0.0	0.0022783666528957227
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACTGG	450195	0.0	43.499702	2
ACTGGGA	105400	0.0	43.473053	4
CACTGGG	444625	0.0	43.410446	3
ACTGGGC	43880	0.0	43.384457	4
GACACTG	454000	0.0	43.36564	1
ACTGGGG	273945	0.0	43.19327	4
CTGGGGG	125330	0.0	42.74443	5
CTGGGAT	32040	0.0	42.48252	5
TGGGGGG	68605	0.0	42.288754	6
CTGGGGC	47875	0.0	42.18068	5
CTGGGGA	67865	0.0	41.86871	5
TGGGATT	10625	0.0	41.692234	6
ACTGGGT	23590	0.0	41.671047	4
CACTAGG	1790	0.0	41.374302	3
CTGGGGT	39100	0.0	41.294117	5
ACACTAG	1910	0.0	41.183247	2
TGGGGCT	26995	0.0	41.126507	6
CTGGGAG	37695	0.0	41.033295	5
CTGGGAC	19460	0.0	40.846867	5
TGGGGAG	29440	0.0	40.734375	6
>>END_MODULE