FastQCFastQC Report
Fri 17 Jun 2016
SRR1527825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975121
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC458372.320718578760491No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG264281.3380446058747795No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT129730.6568205188441619No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT78130.3955707017443489No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC71790.36347140251154236No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC61420.3109682900440024No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG49540.2508200763396268No Hit
TGGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT45440.23006185443828503No Hit
TGGATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG41460.20991119025112892No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC34460.17447032359030157No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC33470.16945797244827027No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC31620.16009145768790875No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT27270.1380674905486803No Hit
TGGATGGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA20840.1055125230302346No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT20450.10353796045913136No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG20260.10257599407833748No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG20110.10181654693560546No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAGGAGCACTGACTGC19950.10100646998335797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG207.857858E-444.024
CGCGATA254.444005E-544.029
ATATGCG207.857858E-444.038
ATCGTTC351.4467878E-744.036
AATAGCG254.444005E-544.036
CGTCATA302.5286445E-644.033
CGATAAC254.444005E-544.041
AATCGAT351.4467878E-744.026
TGGATGG2032500.042.238921
GGATGGG2011700.042.1452522
ATGGGGG1007450.042.139464
GATGGGG1883600.042.1394163
ATGGGGA617750.041.9415634
ATGGGGT102300.041.7204324
TGGGGGG459900.041.679935
TGGGGGA221700.041.3802455
TGGGGAC106300.041.1853265
CGATTAG700.040.85714332
TGGGGGT142050.040.8405535
ATGGGGC165250.040.8048444
Produced by FastQC (version 0.11.5)