##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1975121
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.469718564077844	33.0	31.0	34.0	31.0	34.0
2	32.910246005181456	34.0	31.0	34.0	31.0	34.0
3	33.27733338868859	34.0	34.0	34.0	31.0	34.0
4	35.41682813356751	35.0	35.0	37.0	35.0	37.0
5	36.16513823710041	37.0	35.0	37.0	35.0	37.0
6	36.43973761607517	37.0	37.0	37.0	35.0	37.0
7	36.67011337533245	37.0	37.0	37.0	35.0	37.0
8	36.72710127632687	37.0	37.0	37.0	35.0	37.0
9	38.66930127318782	39.0	39.0	39.0	39.0	39.0
10	38.21610169706059	39.0	39.0	39.0	37.0	39.0
11	37.11782974308916	39.0	35.0	39.0	35.0	39.0
12	36.98758860849538	39.0	35.0	39.0	34.0	39.0
13	36.8589666152099	39.0	35.0	39.0	33.0	39.0
14	37.51587067323977	40.0	35.0	40.0	33.0	40.0
15	37.5954278244219	40.0	35.0	40.0	33.0	40.0
16	37.624373899118076	40.0	35.0	40.0	34.0	40.0
17	37.33802637914335	40.0	35.0	40.0	33.0	40.0
18	37.28308290985717	40.0	35.0	40.0	33.0	40.0
19	37.2751279541861	40.0	35.0	40.0	33.0	40.0
20	37.17451133373601	40.0	35.0	40.0	32.0	40.0
21	37.217240867774684	40.0	35.0	40.0	33.0	40.0
22	37.35835222247143	40.0	35.0	40.0	33.0	40.0
23	37.34351363789864	39.0	35.0	40.0	34.0	40.0
24	37.329219830076234	39.0	35.0	40.0	34.0	40.0
25	37.27443381949764	39.0	35.0	40.0	34.0	40.0
26	37.178941948366706	39.0	35.0	40.0	33.0	40.0
27	37.08797081292741	39.0	35.0	40.0	33.0	40.0
28	36.90069165382779	39.0	35.0	40.0	33.0	40.0
29	36.959674875615214	39.0	35.0	40.0	33.0	40.0
30	37.05400783040634	40.0	35.0	40.0	33.0	40.0
31	37.08227799714549	40.0	35.0	40.0	33.0	40.0
32	37.05566798186035	40.0	35.0	40.0	33.0	40.0
33	36.96171576323678	39.0	35.0	40.0	33.0	40.0
34	36.403561604580176	39.0	35.0	40.0	31.0	40.0
35	36.403878547187745	39.0	35.0	40.0	31.0	40.0
36	36.5240489063708	39.0	35.0	40.0	32.0	40.0
37	36.46891456270274	39.0	35.0	40.0	32.0	40.0
38	36.38972954062055	39.0	35.0	40.0	32.0	40.0
39	36.22804273763481	39.0	35.0	40.0	32.0	40.0
40	36.066987288373724	38.0	35.0	40.0	31.0	40.0
41	35.95940856281717	38.0	35.0	40.0	31.0	40.0
42	35.88983662266767	38.0	35.0	40.0	31.0	40.0
43	35.78683027520846	37.0	35.0	40.0	31.0	40.0
44	35.52250672237296	37.0	35.0	40.0	30.0	40.0
45	35.47213208709745	37.0	35.0	40.0	30.0	40.0
46	35.31743422301722	37.0	35.0	40.0	30.0	40.0
47	35.22572844904185	36.0	35.0	40.0	29.0	40.0
48	35.1616078204829	36.0	35.0	40.0	29.0	40.0
49	35.10534493836074	36.0	35.0	40.0	29.0	40.0
50	34.52986728408032	35.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	8.0
15	33.0
16	79.0
17	196.0
18	432.0
19	769.0
20	1328.0
21	1993.0
22	3175.0
23	4545.0
24	6458.0
25	8980.0
26	11942.0
27	15519.0
28	20673.0
29	25824.0
30	31639.0
31	39992.0
32	52740.0
33	79135.0
34	231811.0
35	178732.0
36	135365.0
37	234920.0
38	551568.0
39	337262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.74549457982575	1.0548720812547687	0.1397889040722062	0.05984443484728277
8	98.59861750242138	1.1864083263759535	0.12196721112276158	0.0930069600799141
9	93.23833830939978	3.59051420140842	1.9678288064376814	1.2033186827541198
10	52.42605389745742	33.04830438236442	6.017200971484785	8.508440748693372
11	38.03513810039993	22.05930674627023	21.611384821486887	18.29417033184296
12	35.82383054000236	16.773655892474434	25.19683604194376	22.205677525579446
13	25.857048758025456	17.28835853600868	27.591119733930224	29.263472972035636
14	23.718951902187257	19.14839647798793	32.63901300224138	24.49363861758343
15	29.317039310503002	19.150117891511456	28.685331177178515	22.84751162080703
16	35.14696061659007	20.72607197229942	23.907851721489468	20.21911568962104
17	32.03702456710247	21.34355312915006	25.072641119202316	21.54678118454515
18	32.27680734496773	21.33038937867604	23.60802198953887	22.784781286817367
19	29.055485714546098	25.735284066140757	22.855511130710475	22.353719088602674
20	31.8070133424737	24.522041940721607	22.527784373716848	21.143160343087843
21	30.459906000695653	25.246301365840374	23.665486823338924	20.62830581012505
22	28.73763177040799	22.072622386172796	27.57127284860016	21.61847299481905
23	31.475084311290296	21.445875974180822	25.447301709616777	21.631738004912105
24	33.881974825846115	20.950311398643425	23.156910386756053	22.01080338875441
25	29.704711761962937	21.879368403252254	20.799637085525394	27.616282749259412
26	26.501161194681238	21.441015512467338	25.67989505453084	26.377928238320592
27	25.04327582968335	20.866265914847748	23.8657277199726	30.22473053549631
28	25.668503347389855	26.216165996918672	21.460963657416432	26.65436699827504
29	31.746206941245624	24.429743797974908	20.482441328910987	23.341607931868477
30	29.233145716135873	21.075316398337115	25.51975296703341	24.171784918493604
31	32.68549116737658	21.515441332455072	22.864877645470834	22.93418985469751
32	35.85901825761561	21.138198621755325	22.317721294037177	20.68506182659189
33	35.808337818290624	20.743994924867895	21.916935721912733	21.530731534928744
34	28.726037544028948	22.602007674466527	28.337706905045312	20.334247876459212
35	25.694780218528386	28.64528299785178	26.480402972779892	19.179533810839942
36	26.442228096405234	30.552153513632835	22.36389568031528	20.641722709646647
37	31.11196731744536	27.731009897621462	20.570081529182264	20.58694125575091
38	29.80419933766083	28.486457285401755	20.55985430766014	21.149489069277276
39	31.1412313473453	24.84425004847804	21.131819265756377	22.882699338420277
40	28.478255256260248	23.197515493987456	20.450443289297212	27.873785960455084
41	23.90648471663255	27.16694318980964	21.778969490983084	27.14760260257473
42	23.515217548697017	24.050678414132605	22.01049960989732	30.423604427273066
43	24.711043019642847	21.764337476033113	22.91449485879599	30.610124645528046
44	25.5661805023591	21.29991023334773	22.289874898803667	30.84403436548951
45	29.29228133364994	22.241574060525913	21.861040412207657	26.60510419361649
46	26.980220452316594	27.15985501647747	21.176373498129987	24.683551033075947
47	24.279474523333	24.11072536821795	23.66108202991108	27.948718078537972
48	25.381533587056186	22.370781334409383	26.917540748136442	25.330144330397985
49	29.238664365373058	21.733959590323835	24.60598616489825	24.421389879404856
50	25.480312345420863	21.8127395739299	24.49900537739207	28.207942703257167
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	6.0
6	12.0
7	38.5
8	65.0
9	374.5
10	684.0
11	1525.0
12	2366.0
13	2674.0
14	2982.0
15	3671.0
16	4360.0
17	4255.0
18	4150.0
19	4301.0
20	4452.0
21	4990.5
22	5529.0
23	5760.0
24	5991.0
25	7120.0
26	8249.0
27	9959.0
28	11669.0
29	14022.0
30	16375.0
31	18309.5
32	20244.0
33	23819.0
34	27394.0
35	31543.0
36	35692.0
37	36536.0
38	37380.0
39	42059.5
40	46739.0
41	49687.5
42	52636.0
43	57309.5
44	61983.0
45	65496.0
46	69009.0
47	72481.5
48	75954.0
49	78241.0
50	80528.0
51	87860.5
52	95193.0
53	99688.5
54	104184.0
55	113126.5
56	122069.0
57	132989.5
58	143910.0
59	152270.0
60	160630.0
61	179214.0
62	197798.0
63	213848.5
64	229899.0
65	173473.0
66	117047.0
67	98233.0
68	79419.0
69	63984.5
70	48550.0
71	43241.5
72	37933.0
73	31439.5
74	24946.0
75	23063.0
76	21180.0
77	15965.0
78	10750.0
79	7648.0
80	4546.0
81	3208.0
82	1870.0
83	1153.0
84	436.0
85	313.5
86	191.0
87	149.5
88	108.0
89	57.5
90	7.0
91	8.0
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1975121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.162525075898843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.90408380502907	6.130103455436166
2	9.56107368726129	1.7520715482409637
3	3.841023326516045	1.0558041763894699
4	1.8298528291551468	0.670642897293539
5	0.9759647619720196	0.44711508023811375
6	0.7107893404699145	0.3907575093442315
7	0.545228626702281	0.34969696749802776
8	0.4394206184330859	0.32209619482081014
9	0.36377934270327134	0.29998236146514506
>10	9.303039167262611	24.32791310507717
>50	3.733186698676689	23.393041340343096
>100	1.6932828490539003	26.833471079624587
>500	0.0729138027085151	4.567728449622278
>1k	0.022995891623454758	3.993081559624457
>5k	0.0016826262163503482	1.0860836499028748
>10k+	0.0016826262163503482	4.380410625079078
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	45837	2.320718578760491	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	26428	1.3380446058747795	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	12973	0.6568205188441619	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	7813	0.3955707017443489	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	7179	0.36347140251154236	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	6142	0.3109682900440024	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	4954	0.2508200763396268	No Hit
TGGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4544	0.23006185443828503	No Hit
TGGATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	4146	0.20991119025112892	No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3446	0.17447032359030157	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	3347	0.16945797244827027	No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3162	0.16009145768790875	No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2727	0.1380674905486803	No Hit
TGGATGGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA	2084	0.1055125230302346	No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2045	0.10353796045913136	No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2026	0.10257599407833748	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2011	0.10181654693560546	No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAGGAGCACTGACTGC	1995	0.10100646998335797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.013973827426269073	0.0	0.0	0.0	0.0
8	0.02485923647209462	0.0	0.0	0.0	0.0
9	0.04136455437413708	0.0	0.0	0.0	0.0
10	0.0815646231294184	0.0	0.0	0.0	0.0
11	0.10920849912486374	0.0	0.0	0.0	0.0
12	0.12647326416963822	0.0	0.0	0.0	0.0
13	0.13756119245352563	0.0	0.0	0.0	0.0
14	0.15431965940314543	0.0	0.0	0.0	0.0
15	0.1726982802572602	0.0	0.0	0.0	0.0
16	0.19234264634926165	0.0	0.0	0.0	0.0
17	0.2114807143461084	0.0	0.0	0.0	0.0
18	0.22793540243863541	0.0	0.0	0.0	0.0
19	0.25228834081557533	0.0	0.0	0.0	0.0
20	0.27421104833577287	0.0	0.0	0.0	0.0
21	0.2955768279513002	0.0	0.0	0.0	0.0
22	0.3160819008050646	0.0	0.0	0.0	0.0
23	0.3412955459437675	0.0	0.0	0.0	0.0
24	0.3655472247016765	0.0	0.0	0.0	0.0
25	0.38671048507914196	0.0	0.0	0.0	0.0
26	0.4095445291706179	0.0	0.0	0.0	0.0
27	0.4272143326915161	0.0	0.0	0.0	0.0
28	0.45121286240184777	0.0	0.0	0.0	0.0
29	0.4694902236369316	0.0	0.0	0.0	0.0
30	0.4886282916337784	0.0	0.0	0.0	0.0
31	0.5040197537264806	0.0	0.0	0.0	0.0
32	0.5193099562001517	0.0	0.0	0.0	0.0
33	0.5341951201976993	0.0	0.0	0.0	0.0
34	0.5489790245762158	0.0	0.0	0.0	0.0
35	0.5728256648580011	0.0	0.0	0.0	0.0
36	0.5914574347596933	0.0	0.0	0.0	0.0
37	0.6087728296139832	0.0	0.0	0.0	0.0
38	0.6259869648492421	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAG	20	7.857858E-4	44.0	24
CGCGATA	25	4.444005E-5	44.0	29
ATATGCG	20	7.857858E-4	44.0	38
ATCGTTC	35	1.4467878E-7	44.0	36
AATAGCG	25	4.444005E-5	44.0	36
CGTCATA	30	2.5286445E-6	44.0	33
CGATAAC	25	4.444005E-5	44.0	41
AATCGAT	35	1.4467878E-7	44.0	26
TGGATGG	203250	0.0	42.23892	1
GGATGGG	201170	0.0	42.145252	2
ATGGGGG	100745	0.0	42.13946	4
GATGGGG	188360	0.0	42.139416	3
ATGGGGA	61775	0.0	41.941563	4
ATGGGGT	10230	0.0	41.720432	4
TGGGGGG	45990	0.0	41.67993	5
TGGGGGA	22170	0.0	41.380245	5
TGGGGAC	10630	0.0	41.185326	5
CGATTAG	70	0.0	40.857143	32
TGGGGGT	14205	0.0	40.840553	5
ATGGGGC	16525	0.0	40.804844	4
>>END_MODULE