FastQCFastQC Report
Fri 17 Jun 2016
SRR1527824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2966322
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC687462.317550151332189No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT342131.1533811905787708No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC194160.6545479553467224No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC109230.3682337925552249No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA106290.3583225287072678No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC88310.2977087450384685No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT74410.2508493683423445No Hit
TGGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT66560.22438561963266293No Hit
TGGATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC63970.21565426814755784No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT59240.19970859535815735No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC51860.1748293003928771No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC48870.16474947763594108No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT45850.15456851953361772No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG41050.13838686427164684No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT31610.10656294225643743No Hit
TGGATGGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT30650.10332661120404327No Hit
TGGATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA30570.10305691694967709No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAGGAGCACTGACTGCTC30040.10127019251450112No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG29710.10015770371524062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG206.3130766E-446.024
CGCGATA600.046.029
ACGCTTA301.862405E-646.033
CGTAACG206.3130766E-446.031
TAGTCGA301.862405E-646.011
TGGATGG3051850.044.1377531
ATGGGGG1499000.044.0605744
GATGGGG2817750.044.025483
GGATGGG3020400.044.0087052
ATGGGGA947200.043.9530224
TGGGGGG693850.043.553655
ATGGGGT145700.042.9375464
TGGGGGA329650.042.9300775
TGGGGGT214350.042.8668065
TGGGGGC291550.042.8286745
TGGGGAC164350.042.781265
TGGGGAG319300.042.4920125
ATGGGGC240100.042.1395264
TGGGGAT309950.041.1692245
TACGCGA402.9126568E-740.2510
Produced by FastQC (version 0.11.5)