##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2966322
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.159660009938236	33.0	31.0	34.0	31.0	34.0
2	32.59578326290942	34.0	31.0	34.0	31.0	34.0
3	33.00939850764684	34.0	31.0	34.0	31.0	34.0
4	35.21989386182619	35.0	35.0	37.0	33.0	37.0
5	35.8646927069954	37.0	35.0	37.0	35.0	37.0
6	36.19449270847871	37.0	37.0	37.0	35.0	37.0
7	36.60464474187226	37.0	37.0	37.0	35.0	37.0
8	36.661395155347265	37.0	37.0	37.0	35.0	37.0
9	38.591998778285024	39.0	39.0	39.0	37.0	39.0
10	37.980286024241465	39.0	38.0	39.0	35.0	39.0
11	36.79392156347153	39.0	35.0	39.0	33.0	39.0
12	36.62296574680699	39.0	35.0	39.0	33.0	39.0
13	36.51254145706366	39.0	35.0	39.0	33.0	39.0
14	37.19297635253354	40.0	35.0	40.0	33.0	40.0
15	37.282885674582865	40.0	35.0	40.0	33.0	40.0
16	37.322991907149664	40.0	35.0	40.0	33.0	40.0
17	37.0976825846958	40.0	35.0	40.0	32.0	40.0
18	37.095155886650204	40.0	35.0	40.0	32.0	40.0
19	37.10876331025425	40.0	35.0	40.0	32.0	40.0
20	37.09624511432002	39.0	35.0	40.0	32.0	40.0
21	37.10695029062927	39.0	35.0	40.0	33.0	40.0
22	37.14896595851698	39.0	35.0	40.0	33.0	40.0
23	37.12650413542427	39.0	35.0	40.0	33.0	40.0
24	37.105313920740905	39.0	35.0	40.0	33.0	40.0
25	37.05591773246465	39.0	35.0	40.0	33.0	40.0
26	36.920021157514256	39.0	35.0	40.0	33.0	40.0
27	36.8376204606243	39.0	35.0	40.0	33.0	40.0
28	36.69478364115561	39.0	35.0	40.0	32.0	40.0
29	36.62346602964884	38.0	35.0	40.0	32.0	40.0
30	36.422162192776106	38.0	35.0	40.0	31.0	40.0
31	36.31220615968192	38.0	35.0	40.0	31.0	40.0
32	36.22783905455982	38.0	35.0	40.0	31.0	40.0
33	36.1202532968437	38.0	35.0	40.0	31.0	40.0
34	35.58069993749836	38.0	34.0	40.0	27.0	40.0
35	35.54073832847546	38.0	34.0	40.0	29.0	40.0
36	35.752440901560924	38.0	34.0	40.0	30.0	40.0
37	35.73409360143639	38.0	34.0	40.0	30.0	40.0
38	35.662610465081	38.0	34.0	40.0	30.0	40.0
39	35.50324172493748	37.0	34.0	40.0	30.0	40.0
40	35.32202808730812	37.0	34.0	40.0	29.0	40.0
41	35.21277797892474	37.0	34.0	40.0	29.0	40.0
42	35.13880927289755	37.0	34.0	40.0	29.0	40.0
43	35.02133820940545	36.0	34.0	40.0	29.0	40.0
44	34.91688663604288	36.0	34.0	40.0	28.0	40.0
45	34.766991580819614	36.0	34.0	40.0	27.0	40.0
46	34.53271020475862	35.0	33.0	40.0	26.0	40.0
47	34.37385084963804	35.0	33.0	40.0	26.0	40.0
48	34.22400467649837	35.0	33.0	39.0	25.0	40.0
49	34.57644348792882	35.0	34.0	40.0	27.0	40.0
50	34.671794228677804	35.0	34.0	40.0	28.0	40.0
51	34.69330672799514	35.0	34.0	40.0	28.0	40.0
52	34.24928750149174	35.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	2.0
14	14.0
15	56.0
16	126.0
17	366.0
18	794.0
19	1632.0
20	2723.0
21	4217.0
22	6668.0
23	9999.0
24	13921.0
25	18993.0
26	25348.0
27	32676.0
28	40842.0
29	49197.0
30	60140.0
31	76733.0
32	102017.0
33	156279.0
34	371540.0
35	246784.0
36	251264.0
37	388095.0
38	770300.0
39	335594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.78401603062647	1.0444247118148333	0.12405935700844345	0.0474999005502437
8	98.62088471851673	1.177485114562748	0.1172158653039016	0.08441430161661478
9	93.3028174284518	3.5427374371359552	1.94719251652383	1.2072526178884153
10	52.36417354555574	33.10355382861335	5.968030443087432	8.56424218274348
11	38.076715879125736	22.013388971258006	21.604026804911943	18.30586834470432
12	35.907598703040335	16.69916482431779	25.221806668325286	22.171429804316592
13	25.90376230227197	17.20271770900125	27.54185149151036	29.351668497216416
14	23.70976583122129	19.024569820808395	32.71307026007291	24.552594087897404
15	29.38005381748846	19.06239443998325	28.657846316077624	22.899705426450666
16	35.19705547813083	20.575042089159574	23.983033534457824	20.244868898251774
17	32.01820301369844	21.236028994829287	25.137695772744834	21.608072218727433
18	32.4523770514462	21.171032679527038	23.61678873702855	22.75980153199821
19	28.949284669702074	25.740428719471453	22.918145771092956	22.392140839733514
20	31.8439131018143	24.39333963069417	22.592557382509384	21.170189884982143
21	30.47393371319769	25.29937747823736	23.606607778926225	20.620081029638722
22	28.71653852818406	21.98139649033382	27.594711565366133	21.707353416115986
23	31.52038113192027	21.35331228369678	25.448113859520312	21.67819272486264
24	33.85620306898577	20.819857048560472	23.253240882142936	22.070699000310825
25	29.72613896940386	21.82692910614559	20.741477155885303	27.705454768565247
26	26.548634976243306	21.331736743347484	25.687433798488495	26.432194481920707
27	25.065249153665718	20.800540197591495	23.844983788004136	30.28922686073865
28	25.661846556105505	26.172917168129423	21.55565039803501	26.609585877730062
29	31.722651822694907	24.344727241344668	20.552927160301547	23.37969377565888
30	29.27837908359241	21.00179953491226	25.521470696707908	24.198350684787425
31	32.82893765410498	21.444468941672547	22.772679432644196	22.95391397157827
32	35.90729529700417	21.029948872711728	22.318952561454893	20.74380326882921
33	35.90513774296924	20.583571169953903	21.888925072868016	21.62236601420884
34	28.639237412526352	22.49068037792256	28.46373387649756	20.406348333053526
35	25.74474382754131	28.581961095255338	26.50339376507338	19.169901312129973
36	26.358669085824125	30.623917430407083	22.392511669333267	20.62490181443552
37	31.16000218452346	27.664933206846726	20.597055882672212	20.5780087259576
38	29.782572492129983	28.509817882212385	20.552724889610772	21.15488473604686
39	31.163440786266627	24.75189814187401	21.186978352316437	22.89768271954292
40	28.376184379173942	23.206786046828363	20.494403507104085	27.922626066893613
41	23.917868660246594	27.133062425454824	21.74686362438063	27.202205289917952
42	23.50577583957507	23.973459388427823	21.971957191431006	30.548807580566102
43	24.687137809044334	21.7766311277063	22.840102996235743	30.69612806701363
44	25.41858233866721	21.16112141567908	22.364160060843023	31.056136184810683
45	29.370412247894865	22.11803034195209	21.85045992983904	26.661097480314005
46	26.914711214763603	27.074606195820955	21.23521991206619	24.775462677349257
47	24.339232220911956	24.007474576259757	23.65002855387918	28.0032646489491
48	25.417368714522564	22.219974770102503	27.02808393694279	25.334572578432144
49	29.298134187724735	21.6195005127562	24.676417462433275	24.405947837085794
50	25.427650807970277	21.730243715955314	24.589441065400184	28.252664410674228
51	23.416169923561906	22.006073514608325	27.575293579051767	27.002462982778
52	23.373726790281026	21.955606977260057	26.211112616904032	28.459553615554885
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	7.0
6	14.0
7	53.5
8	93.0
9	464.0
10	835.0
11	2019.5
12	3204.0
13	3718.5
14	5144.5
15	6056.0
16	6031.5
17	6007.0
18	5813.0
19	5619.0
20	6216.5
21	6814.0
22	6794.5
23	6775.0
24	8163.0
25	9551.0
26	11672.5
27	13794.0
28	16255.5
29	18717.0
30	21529.5
31	24342.0
32	29886.5
33	35431.0
34	38661.0
35	41891.0
36	48148.5
37	54406.0
38	59339.0
39	65462.5
40	66653.0
41	72112.0
42	77571.0
43	84088.0
44	90605.0
45	97182.0
46	103759.0
47	104927.0
48	106095.0
49	114491.5
50	122888.0
51	127883.5
52	132879.0
53	144387.0
54	155895.0
55	164459.0
56	173023.0
57	190100.5
58	207178.0
59	226040.0
60	244902.0
61	262063.5
62	279225.0
63	304162.0
64	253658.5
65	178218.0
66	156670.5
67	135123.0
68	111142.0
69	87161.0
70	73135.0
71	59109.0
72	50607.0
73	42105.0
74	39955.0
75	37805.0
76	28600.0
77	19395.0
78	14817.5
79	10240.0
80	6923.5
81	3607.0
82	2352.5
83	1098.0
84	694.5
85	291.0
86	256.0
87	221.0
88	165.5
89	59.5
90	9.0
91	6.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2966322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.932041047718725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.83085656891855	6.326641183186581
2	9.161035815385304	1.6365349588528584
3	4.050203476988694	1.0852975112422834
4	2.01640340970409	0.7204239209694773
5	1.1568411148551034	0.5166476161787239
6	0.7149986652094854	0.3831838456029134
7	0.5061361473701502	0.3164580190831076
8	0.36320745390774817	0.25953471097131303
9	0.28325310480895216	0.22770255231426015
>10	4.8667905014523996	12.251272902593861
>50	3.659190633606297	23.378263901870607
>100	2.2632922874335213	35.95176781014725
>500	0.08594780838354533	5.161128377656354
>1k	0.037696407185765494	5.577566180771206
>5k	0.0022617844311459295	1.3614701300896688
>10k+	0.0018848203592882746	4.846106378469537
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	68746	2.317550151332189	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	34213	1.1533811905787708	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	19416	0.6545479553467224	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10923	0.3682337925552249	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	10629	0.3583225287072678	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	8831	0.2977087450384685	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	7441	0.2508493683423445	No Hit
TGGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6656	0.22438561963266293	No Hit
TGGATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	6397	0.21565426814755784	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	5924	0.19970859535815735	No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5186	0.1748293003928771	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	4887	0.16474947763594108	No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4585	0.15456851953361772	No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4105	0.13838686427164684	No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	3161	0.10656294225643743	No Hit
TGGATGGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	3065	0.10332661120404327	No Hit
TGGATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	3057	0.10305691694967709	No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAGGAGCACTGACTGCTC	3004	0.10127019251450112	No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2971	0.10015770371524062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.012507071046231664	0.0	0.0	0.0	0.0
8	0.023227417657287374	0.0	0.0	0.0	0.0
9	0.03822916055640622	0.0	0.0	0.0	0.0
10	0.07325570184221403	0.0	0.0	0.0	0.0
11	0.10127019251450112	0.0	0.0	0.0	0.0
12	0.11805865984879592	0.0	0.0	0.0	0.0
13	0.12810477082393618	0.0	0.0	0.0	0.0
14	0.1436121904499916	0.0	0.0	0.0	0.0
15	0.16019838709351175	0.0	0.0	0.0	0.0
16	0.1780656314452713	0.0	0.0	0.0	0.0
17	0.19539348728829845	0.0	0.0	0.0	0.0
18	0.2126876313495298	0.0	0.0	0.0	0.0
19	0.23419574813523278	0.0	0.0	0.0	0.0
20	0.2538160051403725	0.0	0.0	0.0	0.0
21	0.27417792134501917	0.0	0.0	0.0	0.0
22	0.2930228073688561	0.0	0.0	0.0	0.0
23	0.3137555531732563	0.0	0.0	0.0	0.0
24	0.3320947624701566	0.0	0.0	0.0	0.0
25	0.3506362424578316	0.0	0.0	0.0	0.0
26	0.3699530934268094	0.0	0.0	0.0	0.0
27	0.386168460470576	0.0	0.0	0.0	0.0
28	0.40612583529367347	0.0	0.0	0.0	0.0
29	0.4207904603748346	0.0	0.0	0.0	0.0
30	0.4358596268375449	0.0	0.0	0.0	0.0
31	0.4496140338102202	0.0	0.0	0.0	0.0
32	0.4645483531457475	0.0	0.0	0.0	0.0
33	0.47766223626430304	0.0	0.0	3.3711781795772674E-5	0.0
34	0.49027044265592207	0.0	0.0	3.3711781795772674E-5	0.0
35	0.5095535818431041	0.0	0.0	3.3711781795772674E-5	0.0
36	0.5294098213208142	0.0	0.0	3.3711781795772674E-5	0.0
37	0.5449509527286653	0.0	0.0	3.3711781795772674E-5	0.0
38	0.5595481542462349	0.0	0.0	3.3711781795772674E-5	0.0
39	0.5735722554732763	0.0	0.0	3.3711781795772674E-5	0.0
40	0.5896527753898599	0.0	0.0	3.3711781795772674E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAG	20	6.3130766E-4	46.0	24
CGCGATA	60	0.0	46.0	29
ACGCTTA	30	1.862405E-6	46.0	33
CGTAACG	20	6.3130766E-4	46.0	31
TAGTCGA	30	1.862405E-6	46.0	11
TGGATGG	305185	0.0	44.137753	1
ATGGGGG	149900	0.0	44.060574	4
GATGGGG	281775	0.0	44.02548	3
GGATGGG	302040	0.0	44.008705	2
ATGGGGA	94720	0.0	43.953022	4
TGGGGGG	69385	0.0	43.55365	5
ATGGGGT	14570	0.0	42.937546	4
TGGGGGA	32965	0.0	42.930077	5
TGGGGGT	21435	0.0	42.866806	5
TGGGGGC	29155	0.0	42.828674	5
TGGGGAC	16435	0.0	42.78126	5
TGGGGAG	31930	0.0	42.492012	5
ATGGGGC	24010	0.0	42.139526	4
TGGGGAT	30995	0.0	41.169224	5
TACGCGA	40	2.9126568E-7	40.25	10
>>END_MODULE