FastQCFastQC Report
Fri 17 Jun 2016
SRR1527823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998407
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC656662.1900295723695953No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT328221.0946479247146903No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC189850.6331695463624518No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC105310.35121983106362814No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA103960.34671744029412954No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC83900.279815248563654No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT71440.23825984931331873No Hit
TGGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT64810.21614810797867No Hit
TGGATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC60150.20060652206321558No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT57400.19143498531053324No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC50220.16748893662534806No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC47140.15721681546234384No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT44720.14914586311998337No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG39310.13110294899925196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC206.313086E-446.00000446
CGATAAC206.313086E-446.00000441
TACGTTA206.313086E-446.00000432
TGGATGG3086300.044.1428871
GGATGGG3055050.044.006452
GATGGGG2854300.043.9830783
ATGGGGG1526400.043.938684
ATGGGGA942150.043.8224264
TGGGGGG699250.043.4738665
ATGGGGT152950.043.2631574
ATATCGT800.043.12500424
TGGGGAC168000.043.0154765
TGGGGGA331400.042.9324075
TGGGGGC315400.042.733045
ATGGGGC248500.042.5291754
TGGGGAG317750.042.47495
TGGGGGT211350.042.4088025
TGGGGAT303100.041.0676355
TGGGGCA82100.040.369065
CGCGATA402.912675E-740.25000429