##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2998407
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10606832227913	33.0	31.0	34.0	31.0	34.0
2	32.55850289837237	34.0	31.0	34.0	31.0	34.0
3	32.99062168678235	34.0	31.0	34.0	31.0	34.0
4	35.17159444998627	35.0	35.0	37.0	33.0	37.0
5	35.86035518193494	37.0	35.0	37.0	35.0	37.0
6	36.09244475483148	37.0	36.0	37.0	35.0	37.0
7	36.55926597023019	37.0	37.0	37.0	35.0	37.0
8	36.63164773828236	37.0	37.0	37.0	35.0	37.0
9	38.54877273165384	39.0	39.0	39.0	37.0	39.0
10	37.89620855340853	39.0	38.0	39.0	35.0	39.0
11	36.756589415646374	39.0	35.0	39.0	33.0	39.0
12	36.5728098286857	39.0	35.0	39.0	33.0	39.0
13	36.311619469938535	39.0	35.0	39.0	33.0	39.0
14	37.114068570410886	40.0	35.0	40.0	33.0	40.0
15	37.22865641655719	40.0	35.0	40.0	33.0	40.0
16	37.29950203558089	40.0	35.0	40.0	33.0	40.0
17	37.10661361182788	40.0	35.0	40.0	32.0	40.0
18	37.12013712614732	40.0	35.0	40.0	32.0	40.0
19	37.09258416219012	40.0	35.0	40.0	32.0	40.0
20	37.09453253010682	39.0	35.0	40.0	32.0	40.0
21	37.15635635855973	40.0	35.0	40.0	33.0	40.0
22	37.0946529273711	39.0	35.0	40.0	33.0	40.0
23	37.09128947471107	39.0	35.0	40.0	33.0	40.0
24	37.11839286661217	39.0	35.0	40.0	33.0	40.0
25	37.036401662616186	39.0	35.0	40.0	33.0	40.0
26	36.852764484607995	39.0	35.0	40.0	33.0	40.0
27	36.77049413238429	39.0	35.0	40.0	32.0	40.0
28	36.64334995215793	38.0	35.0	40.0	32.0	40.0
29	36.572700437265524	38.0	35.0	40.0	32.0	40.0
30	36.3611954614567	38.0	35.0	40.0	31.0	40.0
31	36.27129305661306	38.0	35.0	40.0	31.0	40.0
32	36.19055952043868	38.0	35.0	40.0	31.0	40.0
33	35.942821638289935	38.0	35.0	40.0	30.0	40.0
34	35.50231506263159	38.0	34.0	40.0	27.0	40.0
35	35.53460387465744	38.0	34.0	40.0	29.0	40.0
36	35.59910912694641	38.0	34.0	40.0	30.0	40.0
37	35.64574455702645	38.0	34.0	40.0	30.0	40.0
38	35.30861921013391	37.0	34.0	40.0	29.0	40.0
39	35.31224546901071	37.0	34.0	40.0	29.0	40.0
40	35.203474044717744	37.0	34.0	40.0	29.0	40.0
41	34.90489049685383	37.0	34.0	40.0	27.0	40.0
42	34.97312072710609	36.0	34.0	40.0	28.0	40.0
43	34.97166528760105	36.0	34.0	40.0	28.0	40.0
44	34.865695350897994	36.0	34.0	40.0	28.0	40.0
45	34.70903383029722	36.0	34.0	40.0	27.0	40.0
46	34.30516737721063	35.0	33.0	40.0	25.0	40.0
47	34.05799246066328	35.0	33.0	39.0	24.0	40.0
48	34.07908899625701	35.0	33.0	39.0	24.0	40.0
49	34.07145060693895	35.0	33.0	39.0	26.0	40.0
50	33.95394254349059	35.0	33.0	39.0	26.0	40.0
51	34.30388536312782	35.0	34.0	39.0	27.0	40.0
52	34.0495533128091	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	12.0
15	50.0
16	141.0
17	433.0
18	871.0
19	1620.0
20	2881.0
21	4751.0
22	7289.0
23	10417.0
24	14687.0
25	19683.0
26	26465.0
27	34333.0
28	42590.0
29	51673.0
30	63785.0
31	81406.0
32	109801.0
33	172281.0
34	372106.0
35	245856.0
36	267955.0
37	415617.0
38	761815.0
39	289880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.7656445572599	1.0555605026268948	0.12726757908449385	0.051527361028706246
8	98.61753257646477	1.179059413882105	0.11552801204106047	0.08787999761206533
9	93.24381246441861	3.555221155767046	1.9835199157419257	1.2174464640724225
10	52.41463216968209	33.0474148439488	5.993515890271067	8.54443709609803
11	38.083188839940675	21.963095737169773	21.59363288572899	18.360082537160565
12	35.91617148705963	16.67785594150494	25.220158570867795	22.185814000567635
13	26.07291138261083	17.25202749326559	27.48939686973783	29.185664254385745
14	23.702752828418557	19.056018745954102	32.716372393741075	24.524856031886266
15	29.417454001408082	19.04104412776518	28.648078796507615	22.89342307431913
16	35.21373182493237	20.566020556915724	23.955420328194272	20.264827289957633
17	31.96547366651692	21.17330969411424	25.132678785768576	21.728537853600262
18	32.41057668288528	21.16760666580621	23.656728389441458	22.76508826186705
19	28.973318165279093	25.70424895619574	22.926907521227104	22.39552535729806
20	31.838206087432425	24.38378112110864	22.550040738298705	21.227972053160226
21	30.449702125161792	25.287594379282062	23.611237567148155	20.651465928407987
22	28.87276477142696	21.979837960623758	27.53088556690269	21.61651170104659
23	31.524172669020583	21.322255450977803	25.420998550230173	21.732573329771444
24	33.8766218195195	20.80438045935725	23.27682666162399	22.04217105949926
25	29.743560497290726	21.784967817911312	20.71489961169381	27.756572073104152
26	26.591119884658752	21.306013493164873	25.721191285906148	26.381675336270227
27	25.10559773906611	20.778766858535217	23.783062139329317	30.332573263069357
28	25.62290576296013	26.181469026719856	21.551043604153804	26.644581606166206
29	31.759164116145673	24.289631127462016	20.548711365735205	23.402493390657106
30	29.299691469503642	21.01129032849777	25.513514342782685	24.17550385921591
31	32.83757008304743	21.402731517102247	22.80057377133925	22.959124628511073
32	35.98420761424316	21.035903398037693	22.27923027127405	20.7006587164451
33	35.8060796949847	20.67004245921251	21.96042765375081	21.56345019205198
34	28.640908322319152	22.47460067962755	28.438634248119083	20.445856749934215
35	25.751940947309688	28.643309597396215	26.413558933126822	19.191190522167272
36	26.588751960624425	30.510367671900447	22.34076294512386	20.56011742235127
37	31.163147631392267	27.65391756355958	20.543075039512647	20.639859765535498
38	29.83497570543292	28.481790497420796	20.46933588402108	21.2138979131252
39	31.184892511256812	24.764650029165487	21.136290036676144	22.91416742290156
40	28.334912505206933	23.21039138449183	20.516761066793133	27.937935043508105
41	24.13178064218767	27.11623205255324	21.719199561633896	27.032787743625196
42	23.45999058833574	23.990972539751944	21.961394833990184	30.587642037922137
43	24.665263921809146	21.705192123684345	22.875113351856502	30.754430602650007
44	25.459418951463224	21.121215365359006	22.33639395852531	31.08297172465246
45	29.414218950262587	22.089129327672993	21.821453858665617	26.675197863398797
46	27.0372901343947	27.03408843429194	21.24698214752033	24.68163928379303
47	24.559174254862665	23.94164634754388	23.63775164612409	27.861427751469364
48	25.43807428411153	22.22836993110008	27.04272635436083	25.290829430427557
49	29.327139377676215	21.57485624866804	24.610868371105056	24.487136002550688
50	25.408525260246524	21.7310058307628	24.53419432385263	28.32627458513804
51	23.424571780949016	22.020159371292824	27.599188502428124	26.956080345330037
52	23.42713981123977	21.894492642259706	26.196376942823306	28.48199060367722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	9.5
6	19.0
7	56.0
8	93.0
9	488.5
10	884.0
11	1941.0
12	2998.0
13	3574.5
14	5056.0
15	5961.0
16	5892.0
17	5823.0
18	5801.0
19	5779.0
20	6353.5
21	6928.0
22	6874.0
23	6820.0
24	8276.5
25	9733.0
26	11853.0
27	13973.0
28	16288.0
29	18603.0
30	21614.5
31	24626.0
32	30165.5
33	35705.0
34	39039.5
35	42374.0
36	48433.0
37	54492.0
38	59420.0
39	65845.5
40	67343.0
41	72858.5
42	78374.0
43	84979.5
44	91585.0
45	98231.5
46	104878.0
47	106024.5
48	107171.0
49	115857.0
50	124543.0
51	129501.0
52	134459.0
53	145683.5
54	156908.0
55	165820.0
56	174732.0
57	192530.0
58	210328.0
59	229216.0
60	248104.0
61	265889.0
62	283674.0
63	306664.5
64	255431.5
65	181208.0
66	159031.0
67	136854.0
68	112781.5
69	88709.0
70	74495.0
71	60281.0
72	51374.5
73	42468.0
74	40372.5
75	38277.0
76	29019.5
77	19762.0
78	15011.5
79	10261.0
80	7017.5
81	3774.0
82	2450.0
83	1126.0
84	708.5
85	291.0
86	258.0
87	225.0
88	156.5
89	49.0
90	10.0
91	6.5
92	3.0
93	4.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2998407.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.353764959139935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.50401824915741	6.594780153775312
2	9.622065580424922	1.8000507972145017
3	4.008204194705538	1.1247539983654309
4	2.100778174199111	0.7860074109099847
5	1.1943281249985678	0.5585732282663451
6	0.7149727239686275	0.4012612087319146
7	0.4815026627378359	0.315270391411482
8	0.3427805564218161	0.2565031005866298
9	0.28490280207127966	0.23984224620976044
>10	5.00234632665893	13.271198319815653
>50	3.543915439829444	23.582692611420235
>100	2.083582056700762	34.69892958942852
>500	0.07897718505641103	5.013355745781352
>1k	0.03352805025979713	5.182358577235975
>5k	0.002235203350653142	1.3517438840799474
>10k+	0.0018626694588776188	4.8226787367669814
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	65666	2.1900295723695953	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	32822	1.0946479247146903	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	18985	0.6331695463624518	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10531	0.35121983106362814	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	10396	0.34671744029412954	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	8390	0.279815248563654	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	7144	0.23825984931331873	No Hit
TGGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6481	0.21614810797867	No Hit
TGGATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	6015	0.20060652206321558	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	5740	0.19143498531053324	No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5022	0.16748893662534806	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	4714	0.15721681546234384	No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4472	0.14914586311998337	No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3931	0.13110294899925196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.01100584410321881	0.0	0.0	0.0	0.0
8	0.021177912138011953	0.0	0.0	0.0	0.0
9	0.034418276104611546	0.0	0.0	0.0	0.0
10	0.06900330742290824	0.0	0.0	0.0	0.0
11	0.09318281340725258	0.0	0.0	0.0	0.0
12	0.11052535563050647	0.0	0.0	0.0	0.0
13	0.1200637538532961	0.0	0.0	0.0	0.0
14	0.1350717230849581	0.0	0.0	0.0	0.0
15	0.15248096739368605	0.0	0.0	0.0	0.0
16	0.1702904242152583	0.0	0.0	0.0	0.0
17	0.18796647686588244	0.0	0.0	0.0	0.0
18	0.20434183884976256	0.0	0.0	0.0	0.0
19	0.22555310203051154	0.0	0.0	0.0	0.0
20	0.2456304297582016	0.0	0.0	0.0	0.0
21	0.2644070668191476	0.0	0.0	0.0	0.0
22	0.28208311946977177	0.0	0.0	0.0	0.0
23	0.30252730866756916	0.0	0.0	0.0	0.0
24	0.32200431762599274	0.0	0.0	0.0	0.0
25	0.34098106094336095	0.0	0.0	0.0	0.0
26	0.3593574854914626	0.0	0.0	0.0	0.0
27	0.37626646415913517	0.0	0.0	0.0	0.0
28	0.3964438450150363	0.0	0.0	0.0	0.0
29	0.41058468713553564	0.0	0.0	0.0	0.0
30	0.4250256886406682	0.0	0.0	0.0	0.0
31	0.43856621199190104	0.0	0.0	0.0	0.0
32	0.452240139514082	0.0	0.0	0.0	0.0
33	0.46421316385667455	0.0	0.0	0.0	0.0
34	0.4786541653618071	0.0	0.0	0.0	0.0
35	0.4954964419440056	0.0	0.0	0.0	0.0
36	0.513539356064737	0.0	0.0	0.0	0.0
37	0.5299814201340912	0.0	0.0	0.0	0.0
38	0.5443223685110127	0.0	0.0	0.0	0.0
39	0.5582631043750899	0.0	0.0	0.0	0.0
40	0.5718369787690597	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	20	6.313086E-4	46.000004	46
CGATAAC	20	6.313086E-4	46.000004	41
TACGTTA	20	6.313086E-4	46.000004	32
TGGATGG	308630	0.0	44.142887	1
GGATGGG	305505	0.0	44.00645	2
GATGGGG	285430	0.0	43.983078	3
ATGGGGG	152640	0.0	43.93868	4
ATGGGGA	94215	0.0	43.822426	4
TGGGGGG	69925	0.0	43.473866	5
ATGGGGT	15295	0.0	43.263157	4
ATATCGT	80	0.0	43.125004	24
TGGGGAC	16800	0.0	43.015476	5
TGGGGGA	33140	0.0	42.932407	5
TGGGGGC	31540	0.0	42.73304	5
ATGGGGC	24850	0.0	42.529175	4
TGGGGAG	31775	0.0	42.4749	5
TGGGGGT	21135	0.0	42.408802	5
TGGGGAT	30310	0.0	41.067635	5
TGGGGCA	8210	0.0	40.36906	5
CGCGATA	40	2.912675E-7	40.250004	29
>>END_MODULE