FastQCFastQC Report
Fri 17 Jun 2016
SRR1527822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807545
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT39630.49074664569776294No Hit
TGTGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC23530.2913769511296584No Hit
TGTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC22350.2767647623352259No Hit
TGTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC19390.24011045824071722No Hit
TGTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC18190.22525060522942997No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16230.2009795119776607No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15810.19577856342371014No Hit
TGTGCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA14300.1770799150511736No Hit
TGTGCGGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAG13600.16841166746125602No Hit
TGTGCGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA13460.1666780179432725No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA13140.1627153904735959No Hit
TGTGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG12870.15937192354605625No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGTGCGGGGA11010.13633915137856092No Hit
TGTGCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC9870.12222229101783802No Hit
TGTGCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG8900.11021057650038078No Hit
TGTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG8620.10674327746441375No Hit
TGTGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8440.10451429951272065Illumina Single End Adapter 2 (97% over 34bp)
TGTGCGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC8280.10253298577788234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG302.5267782E-644.020
CTATGCG207.8550895E-444.027
TCGTTTA254.4416593E-544.032
CTCGTCT302.5267782E-644.037
CTACTAT302.5267782E-644.039
CGTATAA207.8550895E-444.035
AGTCACG302.5267782E-644.038
TTTACGA207.8550895E-444.025
CGAAACG254.4416593E-544.039
TCCGATA302.5267782E-644.020
ACGTTAC254.4416593E-544.033
CCGATAC207.8550895E-444.021
CAACCGT207.8550895E-444.012
ATTCGAG351.445369E-744.017
TACGGGT207.8550895E-444.023
TAACGGC351.445369E-744.032
TTATCGG351.445369E-744.021
GCCATAC302.5267782E-644.025
CTAACGG207.8550895E-444.012
TACGCGG207.8550895E-444.043
Produced by FastQC (version 0.11.5)