##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	807545
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.367360332860706	33.0	31.0	34.0	31.0	34.0
2	32.75101201790612	34.0	31.0	34.0	31.0	34.0
3	31.166951686902898	31.0	31.0	33.0	30.0	34.0
4	35.96521432242166	37.0	35.0	37.0	35.0	37.0
5	36.11406299339356	37.0	37.0	37.0	35.0	37.0
6	36.335627116755106	37.0	37.0	37.0	35.0	37.0
7	36.63337275322118	37.0	37.0	37.0	35.0	37.0
8	36.695397779690296	37.0	37.0	37.0	35.0	37.0
9	38.57821297884328	39.0	39.0	39.0	38.0	39.0
10	38.09037267273031	39.0	39.0	39.0	37.0	39.0
11	37.285254691689005	39.0	37.0	39.0	35.0	39.0
12	37.12979338612709	39.0	37.0	39.0	33.0	39.0
13	37.10997901045762	39.0	37.0	39.0	33.0	39.0
14	37.95938306843581	40.0	37.0	40.0	34.0	40.0
15	38.059401024091535	40.0	37.0	40.0	34.0	40.0
16	38.0367967110192	40.0	37.0	40.0	34.0	40.0
17	37.98483552000198	40.0	37.0	40.0	34.0	40.0
18	37.90625414063613	40.0	37.0	40.0	34.0	40.0
19	37.78372598431047	40.0	36.0	40.0	34.0	40.0
20	37.623889690357814	40.0	36.0	40.0	33.0	40.0
21	37.45051235534862	39.0	35.0	40.0	33.0	40.0
22	37.25026097616851	39.0	35.0	40.0	33.0	40.0
23	37.06936084057235	39.0	35.0	40.0	33.0	40.0
24	36.88364611260054	39.0	35.0	40.0	33.0	40.0
25	36.75353695459696	38.0	35.0	40.0	33.0	40.0
26	36.52033261304324	38.0	35.0	40.0	33.0	40.0
27	36.268695862150096	37.0	35.0	40.0	32.0	40.0
28	35.98697162387236	37.0	35.0	40.0	32.0	40.0
29	35.77465652068925	37.0	35.0	40.0	31.0	40.0
30	35.7588877400021	36.0	35.0	40.0	31.0	40.0
31	35.675263917181084	36.0	35.0	40.0	32.0	40.0
32	35.504119275086836	35.0	35.0	40.0	31.0	40.0
33	35.327034406751324	35.0	35.0	40.0	31.0	40.0
34	34.92507414447492	35.0	35.0	40.0	30.0	40.0
35	34.76953977796903	35.0	35.0	39.0	30.0	40.0
36	34.580087796964875	35.0	35.0	39.0	29.0	40.0
37	34.346258103263594	35.0	34.0	39.0	29.0	40.0
38	34.17186163000204	35.0	34.0	39.0	28.0	40.0
39	33.97452773529648	35.0	34.0	38.0	27.0	40.0
40	33.71364939415141	35.0	34.0	37.0	26.0	40.0
41	33.58403927954479	35.0	34.0	37.0	25.0	40.0
42	33.48070510002538	35.0	34.0	37.0	25.0	40.0
43	33.298482437511225	35.0	34.0	37.0	23.0	40.0
44	33.02842318384734	35.0	34.0	36.0	23.0	40.0
45	33.0094409599465	35.0	34.0	36.0	23.0	39.0
46	32.85813545994341	35.0	34.0	36.0	23.0	39.0
47	32.788084874527115	35.0	33.0	36.0	23.0	39.0
48	32.6361589756608	35.0	33.0	35.0	23.0	39.0
49	32.51133992532924	35.0	33.0	35.0	23.0	39.0
50	31.97274950621947	35.0	33.0	35.0	20.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	2.0
15	11.0
16	39.0
17	106.0
18	221.0
19	511.0
20	780.0
21	1425.0
22	2312.0
23	3626.0
24	5131.0
25	6998.0
26	9260.0
27	12338.0
28	14977.0
29	15282.0
30	15182.0
31	17417.0
32	22250.0
33	33680.0
34	75720.0
35	109768.0
36	161545.0
37	127792.0
38	137295.0
39	33873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.07497415004737	0.7005182373737686	0.1758415939668997	0.04866601861196589
8	98.1158944702772	1.4636955216117986	0.2534843259508758	0.1669256821601273
9	91.52740714139769	4.795646063067693	2.4269854930684978	1.2499613024661165
10	47.71758849352049	35.167328136512516	7.591775071358252	9.523308298608747
11	31.072447975035445	27.69319356816029	22.386616225721166	18.847742231083096
12	29.003461107430546	22.611495334625314	26.12114495167452	22.26389860626962
13	22.321728200905213	20.974806357540444	28.429375452761146	28.274089988793193
14	22.407048523611685	23.683509897281265	28.36015330415023	25.549288274956815
15	25.454680544118286	23.86665758564538	26.80296454067575	23.875697329560584
16	27.740249769362695	23.39671473416342	25.491087183995937	23.37194831247794
17	29.82471565052102	24.185277600629068	22.11220427344606	23.877802475403847
18	27.97255880477249	24.247317486951193	22.540415704387993	25.239708003888328
19	27.051867078614816	23.6244419815614	23.328111746094645	25.99557919372914
20	27.733315171290762	24.990433969623986	23.315480871035053	23.960769988050203
21	29.97517166226031	23.006395928400273	24.54104724814097	22.477385161198445
22	28.282634404274688	22.742138209016215	24.876384597762353	24.098842788946744
23	29.58596734547301	24.461051706096875	22.70585540124699	23.247125547183128
24	27.192911850113617	24.864001386919615	23.294677076819248	24.64840968614752
25	28.851395278281704	24.67899621692909	21.420106619445356	25.04950188534385
26	28.52150654143113	24.344278027849843	21.157582549579278	25.97663288113975
27	29.208774743203165	23.971667213591815	21.418001473602093	25.401556569602935
28	30.840510435950936	25.078850095041144	20.461646100217326	23.618993368790594
29	30.0393166944257	25.44403098279353	21.027558835730517	23.48909348705026
30	28.159545288497856	24.23220996972305	22.03196106718511	25.576283674593984
31	28.71567528744528	25.388430366109628	21.048981790488455	24.846912555956635
32	29.46770768192485	25.365645258158985	21.871598486771635	23.29504857314453
33	30.141602015986724	25.127763777869966	21.579850039316696	23.150784166826615
34	28.372784179209827	25.801534279823418	23.686853364208808	22.13882817675795
35	28.34504578692209	26.011429703607845	23.29405791627711	22.34946659319295
36	28.60323573299321	26.27692574407618	21.68027787925131	23.4395606436793
37	29.17719755555418	26.79256264356785	22.135113213505132	21.89512658737284
38	30.743549895052286	24.837872812041432	21.693032586419335	22.725544706486943
39	28.220099189518848	25.00380783733414	21.90936727984199	24.866725693305018
40	27.809967246407318	25.025726120525793	22.94571819527085	24.218588437796036
41	28.332910240296204	24.668718151929614	22.625364530769183	24.373007077004996
42	27.666322000631542	23.741091827700004	22.89630918400832	25.696276987660134
43	27.01694642403829	23.485997684339573	24.057978193165706	25.43907769845643
44	27.084806419456502	23.408602616572452	24.00658786816834	25.500003095802708
45	27.096446637648675	23.180503872849194	23.38334086645326	26.339708623048868
46	27.61183587292349	24.279018506708606	23.68858701372679	24.420558606641116
47	27.536545950999635	23.76969704474673	23.87247769474147	24.821279309512164
48	28.975475050925954	23.425939111752285	23.397333894705557	24.2012519426162
49	26.7032796933917	23.60103771306862	24.30390876050251	25.391773833037167
50	25.064609402571993	24.52383458506956	24.56866180832028	25.842894204038164
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	8.0
8	16.0
9	44.0
10	72.0
11	598.0
12	1124.0
13	1725.5
14	2327.0
15	1880.5
16	1434.0
17	1723.5
18	2013.0
19	2191.5
20	2370.0
21	2344.0
22	2318.0
23	2598.5
24	2879.0
25	3824.5
26	4770.0
27	5620.0
28	6470.0
29	7342.0
30	8214.0
31	8660.0
32	9106.0
33	12150.0
34	15194.0
35	14180.0
36	13166.0
37	13274.5
38	13383.0
39	15944.0
40	18505.0
41	18576.0
42	18647.0
43	19783.0
44	20919.0
45	22474.5
46	24030.0
47	25023.5
48	26017.0
49	27969.0
50	29921.0
51	32027.0
52	34133.0
53	36131.0
54	38129.0
55	41045.5
56	43962.0
57	44503.5
58	45045.0
59	55386.5
60	65728.0
61	65196.0
62	64664.0
63	67754.5
64	70845.0
65	65979.5
66	61114.0
67	56502.0
68	51890.0
69	43245.5
70	34601.0
71	29638.0
72	24675.0
73	20678.0
74	16681.0
75	14963.5
76	13246.0
77	12780.5
78	12315.0
79	8569.5
80	4824.0
81	3147.5
82	1471.0
83	1130.0
84	789.0
85	592.0
86	395.0
87	227.0
88	59.0
89	61.0
90	63.0
91	40.0
92	17.0
93	8.5
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	807545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.006004965314775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89550993589864	10.489868840419787
2	8.429507229733451	2.3612744022960706
3	3.119680904814329	1.3108279872908155
4	1.5294682180742094	0.8568695782655407
5	0.8551039877666361	0.5988295349259986
6	0.5528530579735932	0.4645957605040561
7	0.37330606273776995	0.3659968597801121
8	0.31464330317491873	0.35255165332567634
9	0.2426486064964721	0.305868382867467
>10	5.762237615741426	22.779413307956112
>50	2.680689286686379	25.99955869604032
>100	1.2043547690517387	28.54559857351956
>500	0.028442326649192353	2.6618142313837816
>1k	0.011554695201234392	2.906932191424688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3963	0.49074664569776294	No Hit
TGTGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2353	0.2913769511296584	No Hit
TGTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2235	0.2767647623352259	No Hit
TGTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	1939	0.24011045824071722	No Hit
TGTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1819	0.22525060522942997	No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1623	0.2009795119776607	No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1581	0.19577856342371014	No Hit
TGTGCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	1430	0.1770799150511736	No Hit
TGTGCGGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAG	1360	0.16841166746125602	No Hit
TGTGCGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA	1346	0.1666780179432725	No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1314	0.1627153904735959	No Hit
TGTGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	1287	0.15937192354605625	No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGTGCGGGGA	1101	0.13633915137856092	No Hit
TGTGCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	987	0.12222229101783802	No Hit
TGTGCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	890	0.11021057650038078	No Hit
TGTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	862	0.10674327746441375	No Hit
TGTGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	844	0.10451429951272065	Illumina Single End Adapter 2 (97% over 34bp)
TGTGCGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	828	0.10253298577788234	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.019317808914673485	0.0	0.0	0.0	0.0
8	0.027366895962454105	0.0	0.0	0.0	0.0
9	0.03504448668495254	0.0	0.0	0.0	0.0
10	0.07727123565869394	0.0	0.0	0.0	0.0
11	0.1056287884885672	0.0	0.0	0.0	0.0
12	0.14215926047464847	0.0	0.0	0.0	0.0
13	0.16754484270226427	0.0	0.0	0.0	0.0
14	0.2090285990254413	0.0	0.0	0.0	0.0
15	0.2534843259508758	0.0	0.0	0.0	0.0
16	0.2937297611897789	0.0	0.0	0.0	0.0
17	0.3290219120915862	0.0	0.0	0.0	0.0
18	0.36864818678835237	0.0	0.0	0.0	0.0
19	0.41545672377390735	0.0	0.0	0.0	0.0
20	0.46251292497631713	0.0	0.0	0.0	0.0
21	0.5145224105158226	0.0	0.0	0.0	0.0
22	0.5709898519587143	0.0	0.0	0.0	0.0
23	0.6291909429195897	0.0	0.0	0.0	0.0
24	0.6898686760490127	0.0	0.0	0.0	0.0
25	0.7375440377935595	0.0	0.0	0.0	0.0
26	0.7910395086341938	0.0	0.0	0.0	0.0
27	0.8356190676680556	0.0	0.0	0.0	0.0
28	0.8834182615210298	0.0	0.0	0.0	0.0
29	0.9231683683262233	0.0	0.0	0.0	0.0
30	0.9569745339269019	0.0	0.0	0.0	0.0
31	0.9859512472989121	0.0	0.0	0.0	0.0
32	1.0165377780804785	0.0	0.0	0.0	0.0
33	1.038084564946845	0.0	0.0	0.0	0.0
34	1.062850986632324	0.0	0.0	0.0	0.0
35	1.101858100786953	0.0	0.0	0.0	0.0
36	1.126005361930295	0.0	0.0	0.0	0.0
37	1.152753097350612	0.0	0.0	0.0	0.0
38	1.177519519036091	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	30	2.5267782E-6	44.0	20
CTATGCG	20	7.8550895E-4	44.0	27
TCGTTTA	25	4.4416593E-5	44.0	32
CTCGTCT	30	2.5267782E-6	44.0	37
CTACTAT	30	2.5267782E-6	44.0	39
CGTATAA	20	7.8550895E-4	44.0	35
AGTCACG	30	2.5267782E-6	44.0	38
TTTACGA	20	7.8550895E-4	44.0	25
CGAAACG	25	4.4416593E-5	44.0	39
TCCGATA	30	2.5267782E-6	44.0	20
ACGTTAC	25	4.4416593E-5	44.0	33
CCGATAC	20	7.8550895E-4	44.0	21
CAACCGT	20	7.8550895E-4	44.0	12
ATTCGAG	35	1.445369E-7	44.0	17
TACGGGT	20	7.8550895E-4	44.0	23
TAACGGC	35	1.445369E-7	44.0	32
TTATCGG	35	1.445369E-7	44.0	21
GCCATAC	30	2.5267782E-6	44.0	25
CTAACGG	20	7.8550895E-4	44.0	12
TACGCGG	20	7.8550895E-4	44.0	43
>>END_MODULE