FastQCFastQC Report
Fri 17 Jun 2016
SRR1527821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209240
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG58830.4865039198174059No Hit
TGTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC34840.28811484899606365No Hit
TGTGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32700.27041778307035824No Hit
TGTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG29500.24395488075154645No Hit
TGTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC26740.22113062750157123No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC25440.21038007343455395No Hit
TGTGCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA21910.18118818431411465No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20880.1726704376302471No Hit
TGTGCGGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA20750.17159538222354537No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC20270.1676259468757236No Hit
TGTGCGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAATA20070.16597201548079787No Hit
TGTGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC18600.15381561972809368No Hit
TGTGCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC14670.12131586781780292No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGTGCGGGGAAG14410.11916575700439945No Hit
TGTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC12550.10378419503159009No Hit
TGTGCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG12460.10303992590387351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG301.8614828E-646.046
AAGTACG206.311586E-446.022
CGTATAT206.311586E-446.016
CGAGTAT453.092282E-1046.08
TCCGATA453.092282E-1046.020
TAGACGG206.311586E-446.021
CGTTGTC301.8614828E-646.030
GACGTAT206.311586E-446.014
CGTCTAC301.8614828E-646.014
CACGACG206.311586E-446.021
TAACGAC253.417261E-546.011
ATGTCGC253.417261E-546.021
GCGTTAG501.6370905E-1146.032
TCGATAG453.092282E-1046.039
TTATCCG405.6115823E-946.023
TCTAGCG453.092282E-1046.026
ATCGTTA206.311586E-446.012
CCGTCAA206.311586E-446.035
AGTATCG405.6115823E-946.036
CTAACGG206.311586E-446.012
Produced by FastQC (version 0.11.5)