FastQCFastQC Report
Fri 17 Jun 2016
SRR1527820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222314
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG56510.46231982943826216No Hit
TGTGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT33730.27595200578574736No Hit
TGTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC33490.27398851686227926No Hit
TGTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG27720.22678297066056674No Hit
TGTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC25550.20902975831087592No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC25040.20485734434850617No Hit
TGTGCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA21970.17974104853580997No Hit
TGTGCGGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA21230.17368695768844994No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20150.16485125753284344No Hit
TGTGCGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAATA19680.16100609172438504No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC19060.1559337453387591No Hit
TGTGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC17620.14415281179795045No Hit
TGTGCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC13850.11330967329180554No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGTGCGGGGAAG12240.10013793509687365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG253.4172837E-546.046
AACGTGT253.4172837E-546.039
ACTATCG253.4172837E-546.034
ACGTTTA206.311612E-446.043
TTTACGA301.861501E-646.025
TAAAGCG206.311612E-446.031
TAGACCC301.861501E-646.013
CGCATAT206.311612E-446.042
TTCACGC301.861501E-646.034
TCGGGTA405.6115823E-946.036
CGATTTA301.861501E-646.037
CACGATT501.6370905E-1146.021
GCGTTAG206.311612E-446.032
TTATCCG501.6370905E-1146.023
ATCGTTG206.311612E-446.030
ATGTACG600.046.031
ACGATTG501.6370905E-1146.022
CTAACGA850.046.013
GTTAACG206.311612E-446.036
CGAATAT600.046.029
Produced by FastQC (version 0.11.5)