##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222314
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00499789743061	33.0	31.0	34.0	31.0	34.0
2	32.410822423698	34.0	31.0	34.0	31.0	34.0
3	30.88437668226004	31.0	31.0	31.0	30.0	33.0
4	35.75566180212286	37.0	35.0	37.0	35.0	37.0
5	35.909767866522024	37.0	35.0	37.0	35.0	37.0
6	36.03575840577789	37.0	35.0	37.0	35.0	37.0
7	36.534871563280795	37.0	37.0	37.0	35.0	37.0
8	36.59807872608839	37.0	37.0	37.0	35.0	37.0
9	38.45593358171468	39.0	39.0	39.0	37.0	39.0
10	37.76315169424551	39.0	38.0	39.0	35.0	39.0
11	36.86647211763917	39.0	35.0	39.0	33.0	39.0
12	36.6728189319602	39.0	35.0	39.0	32.0	39.0
13	36.511897106635445	39.0	35.0	39.0	33.0	39.0
14	37.5224770394514	40.0	36.0	40.0	33.0	40.0
15	37.66255806609431	40.0	36.0	40.0	33.0	40.0
16	37.67285574737752	40.0	36.0	40.0	33.0	40.0
17	37.64902553680969	40.0	36.0	40.0	33.0	40.0
18	37.634510444942954	40.0	36.0	40.0	33.0	40.0
19	37.468395191415624	40.0	35.0	40.0	33.0	40.0
20	37.395107149226796	39.0	35.0	40.0	33.0	40.0
21	37.27714400718637	39.0	35.0	40.0	33.0	40.0
22	36.911423742180816	39.0	35.0	40.0	33.0	40.0
23	36.76738137663481	39.0	35.0	40.0	32.0	40.0
24	36.61577876061307	38.0	35.0	40.0	32.0	40.0
25	36.45075651591981	38.0	35.0	40.0	32.0	40.0
26	36.143539221509364	37.0	35.0	40.0	31.0	40.0
27	35.923744635175574	37.0	35.0	40.0	31.0	40.0
28	35.69592592410788	37.0	35.0	40.0	31.0	40.0
29	35.41826813731987	36.0	35.0	40.0	30.0	40.0
30	35.1415380990482	35.0	34.0	39.0	30.0	40.0
31	34.95589103945468	35.0	34.0	39.0	29.0	40.0
32	34.670354753361245	35.0	34.0	39.0	29.0	40.0
33	34.3454014271292	35.0	34.0	39.0	27.0	40.0
34	34.21355069155716	35.0	33.0	39.0	27.0	40.0
35	34.050271861403864	35.0	33.0	39.0	27.0	40.0
36	33.763834824766796	35.0	33.0	38.0	26.0	40.0
37	33.575789854325485	35.0	33.0	38.0	25.0	40.0
38	33.15920377251672	35.0	33.0	37.0	23.0	40.0
39	33.111275826015245	35.0	33.0	37.0	23.0	40.0
40	32.89766132106807	35.0	33.0	37.0	23.0	40.0
41	32.60255793519505	35.0	33.0	37.0	22.0	40.0
42	32.625542209285015	35.0	33.0	36.0	22.0	40.0
43	32.53346357809859	35.0	33.0	36.0	22.0	39.0
44	32.39091591849557	35.0	32.0	36.0	22.0	39.0
45	32.31311512426431	35.0	32.0	36.0	22.0	39.0
46	32.02019448357787	35.0	32.0	35.0	20.0	39.0
47	31.798465860654463	35.0	32.0	35.0	20.0	39.0
48	31.692882516276505	35.0	31.0	35.0	20.0	38.0
49	31.609438327631036	35.0	31.0	35.0	20.0	38.0
50	31.501542156925307	35.0	31.0	35.0	20.0	37.0
51	31.74468589904067	35.0	32.0	35.0	20.0	37.0
52	31.49331595645636	35.0	32.0	35.0	20.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	3.0
14	11.0
15	18.0
16	64.0
17	222.0
18	499.0
19	1033.0
20	1981.0
21	3296.0
22	5238.0
23	7804.0
24	10996.0
25	14783.0
26	19168.0
27	23227.0
28	25003.0
29	25686.0
30	29255.0
31	36502.0
32	49152.0
33	74623.0
34	140355.0
35	176437.0
36	224970.0
37	167666.0
38	162225.0
39	22095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.10751247224526	0.6868120630214495	0.15928803891635046	0.046387425816934114
8	98.14180317005287	1.4252475223224146	0.26057134255191383	0.17237796507280453
9	91.631282960025	4.719981935901904	2.394392930130883	1.2543421739422114
10	47.772421816325426	35.17287701850752	7.4877650096456385	9.566936155521413
11	31.046686857877763	27.492608282323527	22.569732490996586	18.890972368802124
12	29.251076237366174	22.168689878378224	26.219203903415977	22.361029980839618
13	22.665861636208046	20.56468305198173	28.505686754794596	28.263768557015627
14	22.47843025605532	23.437103722938623	28.44007349993537	25.644392521070692
15	25.485268106231295	23.543868433152202	26.987828004915265	23.983035455701234
16	27.877124863169367	23.03581567420483	25.56822551324782	23.51883394937798
17	29.95850493408404	23.759524966579782	22.28829907863282	23.993671020703356
18	28.15594029030184	23.828656139093553	22.687296390289237	25.328107180315367
19	27.127153906443024	23.29311453521763	23.57945666989006	26.000274888449287
20	27.864443997205303	24.807128119288496	23.309967815144063	24.01846006836214
21	29.954823392352537	22.763872458304494	24.707071996230102	22.574232153112867
22	28.440891620320148	22.337795361911912	24.935000335429358	24.286312682338583
23	29.55288084731092	24.19165615381972	22.78890694207871	23.466556056790644
24	27.16601462472	24.59253514236113	23.424913729205425	24.81653650371345
25	28.798082980314387	24.489288349802095	21.45937950477537	25.253249165108148
26	28.63666782839761	24.071310645218823	21.236932572154128	26.05508895422944
27	29.249930868827484	23.758870470271958	21.46698802435381	25.524210636546744
28	30.82890321144976	24.900230219076278	20.46299068815378	23.807875881320186
29	30.14724530685241	25.124722452659466	21.106115122628065	23.621917117860058
30	28.306310817024105	24.011178796937614	22.07305160539763	25.609458780640654
31	28.778448091079706	25.185263361133064	21.101942708665693	24.934345839121537
32	29.693106681261934	25.15973800512798	21.879075262166676	23.26808005144341
33	30.243210827986918	24.853597357143908	21.72453232148204	23.178659493387133
34	28.32169148025794	25.6144493149878	23.79028629304745	22.27357291170681
35	28.392295269464313	25.891710313389193	23.300395806642154	22.41559861050434
36	28.95949813223116	26.023345883300035	21.75553908406514	23.261616900403663
37	29.171882184119628	26.665079513120197	22.204605363269994	21.95843293949018
38	30.74414593958672	24.775466860397575	21.610813587997846	22.86957361201786
39	28.20772731065831	24.854497289567163	21.93920711044789	24.998568289326638
40	27.796458193230215	24.987278228016695	22.88209085390497	24.334172724848116
41	28.497423738908335	24.569545959548854	22.81549585458401	24.117534446958803
42	27.746471037720262	23.597128070201272	22.774180775152704	25.882220116925765
43	26.997236389340216	23.455838679750048	24.069674404449266	25.477250526460466
44	27.002063299610413	23.279615548868783	24.096508753069998	25.621812398450807
45	27.208066012497607	22.96881161469148	23.365109129078128	26.458013243732786
46	27.603299970384043	24.10509901711017	23.792331594009394	24.499269418496393
47	27.876388554823066	23.62273523824484	23.929203134382817	24.57167307254928
48	29.19274425393148	23.257362674402813	23.350301150113637	24.19959192155207
49	26.89055349116512	23.467619613290857	24.30594757157326	25.33587932397076
50	25.26396654214874	24.415575703133566	24.37908753397245	25.94137022074524
51	26.063270158077223	24.091681842799805	24.196810312243827	25.64823768687915
52	27.046814484657787	23.995143637395955	23.438985399823615	25.519056478122643
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	7.5
8	15.0
9	48.0
10	81.0
11	753.0
12	1425.0
13	2250.0
14	2477.5
15	1880.0
16	2321.0
17	2762.0
18	2887.5
19	3013.0
20	2983.0
21	2953.0
22	3125.0
23	3297.0
24	4001.0
25	4705.0
26	5774.0
27	6843.0
28	8057.0
29	9271.0
30	10187.0
31	11103.0
32	15668.5
33	20234.0
34	18828.5
35	17423.0
36	19305.0
37	21187.0
38	21980.5
39	25457.0
40	28140.0
41	28185.5
42	28231.0
43	29743.5
44	31256.0
45	33500.5
46	35745.0
47	38749.0
48	41753.0
49	41491.0
50	41229.0
51	46270.5
52	51312.0
53	52141.5
54	52971.0
55	58479.5
56	63988.0
57	66642.5
58	69297.0
59	75715.5
60	82134.0
61	94081.0
62	106028.0
63	101317.5
64	95834.5
65	95062.0
66	85759.5
67	76457.0
68	70050.0
69	63643.0
70	51754.0
71	39865.0
72	33346.0
73	26827.0
74	24599.5
75	22372.0
76	21519.5
77	20667.0
78	15615.0
79	10563.0
80	6853.5
81	3144.0
82	2461.0
83	1778.0
84	1320.0
85	862.0
86	538.0
87	214.0
88	144.5
89	54.5
90	34.0
91	25.5
92	17.0
93	8.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1222314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.07693363058004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12399690916567	10.856693858153852
2	8.329473324778078	2.3450688634117576
3	3.304120331042567	1.3953564782260908
4	1.7375467292793427	0.9783731995238693
5	0.9294054424060396	0.6541589364324849
6	0.5304912669132081	0.44806142135637317
7	0.37309726135588567	0.36764457701005837
8	0.26986271759635605	0.3039071651977495
9	0.21820498088239684	0.27644913303691376
>10	3.215521750373825	12.309356457224562
>50	2.335945989650835	23.69242022195421
>100	1.5691345516670259	38.049249581316985
>500	0.04995710310255586	4.6205595427657755
>1k	0.0126397489777551	3.2239023472006045
>5k	6.018928084645285E-4	0.47879821718871524
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5651	0.46231982943826216	No Hit
TGTGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3373	0.27595200578574736	No Hit
TGTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3349	0.27398851686227926	No Hit
TGTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	2772	0.22678297066056674	No Hit
TGTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2555	0.20902975831087592	No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2504	0.20485734434850617	No Hit
TGTGCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2197	0.17974104853580997	No Hit
TGTGCGGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA	2123	0.17368695768844994	No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2015	0.16485125753284344	No Hit
TGTGCGGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAATA	1968	0.16100609172438504	No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1906	0.1559337453387591	No Hit
TGTGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1762	0.14415281179795045	No Hit
TGTGCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1385	0.11330967329180554	No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGTGCGGGGAAG	1224	0.10013793509687365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.015544287310789207	0.0	0.0	0.0	0.0
8	0.022825558735316786	0.0	0.0	0.0	0.0
9	0.03329749966048004	0.0	0.0	0.0	0.0
10	0.07207640589897522	0.0	0.0	0.0	0.0
11	0.09784719801949417	0.0	0.0	0.0	0.0
12	0.12689047167912665	0.0	0.0	0.0	0.0
13	0.14767072945249748	0.0	0.0	0.0	0.0
14	0.18514064307534725	0.0	0.0	0.0	0.0
15	0.22220149650580784	0.0	0.0	0.0	0.0
16	0.251899266473263	0.0	0.0	0.0	0.0
17	0.2832332772102749	0.0	0.0	0.0	0.0
18	0.31972144637139066	0.0	0.0	0.0	0.0
19	0.3588276007637972	0.0	0.0	0.0	0.0
20	0.40030630427206104	0.0	0.0	0.0	0.0
21	0.4464482939735616	0.0	0.0	0.0	0.0
22	0.4901359225207271	0.0	0.0	0.0	0.0
23	0.5402867021076417	0.0	0.0	0.0	0.0
24	0.587083188116965	0.0	0.0	0.0	0.0
25	0.630525380548697	0.0	0.0	0.0	0.0
26	0.6729858285186948	0.0	0.0	0.0	0.0
27	0.7035835309094063	0.0	0.0	0.0	0.0
28	0.74506223441767	0.0	0.0	0.0	0.0
29	0.7682968533453761	0.0	0.0	0.0	0.0
30	0.7933313371195945	0.0	0.0	0.0	0.0
31	0.8132116624697091	0.0	0.0	0.0	0.0
32	0.8348918526663361	0.0	0.0	0.0	0.0
33	0.8547721780164508	0.0	0.0	0.0	0.0
34	0.8759614959822107	0.0	0.0	0.0	0.0
35	0.9104043641813806	0.0	0.0	0.0	0.0
36	0.9292211330312833	0.0	0.0	0.0	0.0
37	0.9468107213040184	0.0	0.0	0.0	0.0
38	0.9642366854997979	0.0	0.0	0.0	0.0
39	0.9804354691184098	0.0	0.0	0.0	0.0
40	0.9996612981607017	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	25	3.4172837E-5	46.0	46
AACGTGT	25	3.4172837E-5	46.0	39
ACTATCG	25	3.4172837E-5	46.0	34
ACGTTTA	20	6.311612E-4	46.0	43
TTTACGA	30	1.861501E-6	46.0	25
TAAAGCG	20	6.311612E-4	46.0	31
TAGACCC	30	1.861501E-6	46.0	13
CGCATAT	20	6.311612E-4	46.0	42
TTCACGC	30	1.861501E-6	46.0	34
TCGGGTA	40	5.6115823E-9	46.0	36
CGATTTA	30	1.861501E-6	46.0	37
CACGATT	50	1.6370905E-11	46.0	21
GCGTTAG	20	6.311612E-4	46.0	32
TTATCCG	50	1.6370905E-11	46.0	23
ATCGTTG	20	6.311612E-4	46.0	30
ATGTACG	60	0.0	46.0	31
ACGATTG	50	1.6370905E-11	46.0	22
CTAACGA	85	0.0	46.0	13
GTTAACG	20	6.311612E-4	46.0	36
CGAATAT	60	0.0	46.0	29
>>END_MODULE