FastQCFastQC Report
Fri 17 Jun 2016
SRR1527819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175778
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA202320.9298742794531427No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135900.6246041645792907No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGA130550.6000152589096865No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA106640.4901235328236613No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84420.3879991432949501No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG78230.3595495496323614No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC60220.27677456063991823No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA42150.19372380821940474No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAA39860.18319883738138726No Hit
GCTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA38260.17584514596617853Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT34930.1605402757082754No Hit
GCTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA34600.1590235768538886Illumina Single End Adapter 2 (100% over 34bp)
GCTGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA32120.14762535516031505Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA27570.12671329519831526No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT27540.12657541348428009No Hit
GCTGTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC27160.12482891177316804No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25530.11733733864392414No Hit
GCTGTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG24200.1112245826550319No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAA24070.1106270952275462No Hit
GCTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA23840.10957000208660994Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC23470.10786946094684291No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGG22740.10451433923865394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA207.858032E-444.00000438
ATACTCG408.3182385E-944.00000444
TACGTTC207.858032E-444.00000414
TACCGTA207.858032E-444.00000419
CGACGTA302.5287627E-644.028
CGCGATT254.444155E-544.013
TACGCTT351.4468787E-744.023
CATACGC351.4468787E-744.020
ATAACCG254.444155E-544.029
TAATCGA254.444155E-544.042
TGGGGAT195200.039.3452875
AAGCAGT84650.038.88009610
TGGGGGT170400.038.7582175
TGGGGAC91800.038.6078455
GTGGGGG977950.038.5109674
CGTCTAT404.1267958E-738.50000434
TGGGGGG255200.038.4741365
TGGGGAG263800.037.803645
ACGTACG357.293207E-637.71428730
TATCCGA357.293207E-637.71428711
Produced by FastQC (version 0.11.5)