##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527819_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2175778
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.161846015540185	33.0	31.0	34.0	31.0	34.0
2	32.59978545605296	34.0	31.0	34.0	31.0	34.0
3	32.63481292668646	34.0	31.0	34.0	31.0	34.0
4	36.27636826918923	37.0	37.0	37.0	35.0	37.0
5	35.09485894241048	35.0	35.0	37.0	33.0	37.0
6	36.03344734619065	37.0	35.0	37.0	35.0	37.0
7	36.539747161704916	37.0	37.0	37.0	35.0	37.0
8	36.62941118073627	37.0	37.0	37.0	35.0	37.0
9	38.52559130573064	39.0	39.0	39.0	37.0	39.0
10	37.89021766007377	39.0	38.0	39.0	35.0	39.0
11	37.2539243433843	39.0	37.0	39.0	35.0	39.0
12	37.16331399618895	39.0	37.0	39.0	34.0	39.0
13	37.01433418299109	39.0	37.0	39.0	33.0	39.0
14	37.882373569362315	40.0	37.0	40.0	33.0	40.0
15	38.00484470382548	40.0	38.0	40.0	34.0	40.0
16	37.913724653893915	40.0	37.0	40.0	34.0	40.0
17	37.801041742310105	40.0	37.0	40.0	34.0	40.0
18	37.816070389534225	40.0	37.0	40.0	34.0	40.0
19	37.69375735943649	40.0	37.0	40.0	33.0	40.0
20	37.78398301664968	40.0	37.0	40.0	33.0	40.0
21	37.76666001770401	40.0	37.0	40.0	33.0	40.0
22	37.61621406228025	40.0	37.0	40.0	33.0	40.0
23	37.4270536791897	39.0	36.0	40.0	33.0	40.0
24	37.18889059453676	39.0	36.0	40.0	33.0	40.0
25	36.85547836222262	39.0	35.0	40.0	33.0	40.0
26	36.65029106829833	38.0	35.0	40.0	32.0	40.0
27	36.566109226217016	39.0	35.0	40.0	32.0	40.0
28	36.38767374244983	38.0	35.0	40.0	31.0	40.0
29	36.29390176755165	39.0	35.0	40.0	31.0	40.0
30	36.20984953428153	39.0	35.0	40.0	31.0	40.0
31	36.05594688428691	39.0	35.0	40.0	30.0	40.0
32	35.75724683308683	38.0	35.0	40.0	30.0	40.0
33	35.54267530970531	38.0	35.0	40.0	28.0	40.0
34	34.955308859635494	38.0	35.0	40.0	24.0	40.0
35	34.79293475713055	38.0	35.0	40.0	23.0	40.0
36	34.455745485063275	38.0	34.0	40.0	21.0	40.0
37	33.98709289274917	38.0	34.0	40.0	17.0	40.0
38	33.69294661495796	38.0	34.0	40.0	15.0	40.0
39	33.32648689342387	37.0	33.0	40.0	12.0	40.0
40	32.85954771120951	36.0	33.0	40.0	10.0	40.0
41	32.62830812702399	35.0	33.0	40.0	10.0	40.0
42	32.417224551401844	35.0	33.0	40.0	10.0	40.0
43	32.19439988822389	35.0	32.0	40.0	10.0	40.0
44	31.912247021525175	35.0	31.0	40.0	10.0	40.0
45	31.77939201517802	35.0	31.0	40.0	9.0	40.0
46	31.547393621959593	35.0	31.0	40.0	9.0	40.0
47	31.40935885922185	35.0	31.0	40.0	9.0	40.0
48	31.20241862910646	35.0	30.0	39.0	9.0	40.0
49	30.971523289600317	35.0	29.0	39.0	9.0	40.0
50	30.201060494223217	35.0	27.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	12.0
14	15.0
15	52.0
16	130.0
17	307.0
18	649.0
19	1399.0
20	2689.0
21	4848.0
22	8306.0
23	14145.0
24	22352.0
25	31503.0
26	41717.0
27	55851.0
28	69936.0
29	71984.0
30	62760.0
31	58279.0
32	67101.0
33	88594.0
34	166430.0
35	158744.0
36	197297.0
37	317741.0
38	491213.0
39	241717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.964784091024	0.6309467234249082	0.29120618004226534	0.1130630055088341
8	98.27252596542479	0.9705034245221709	0.44926458489790777	0.3077060251551399
9	92.19276966675828	4.358211177794794	1.96072393415137	1.4882952212955551
10	42.32706645622853	43.77831745701997	6.544969201821142	7.349646884930356
11	25.81044573481302	37.146988341641475	19.512146919400784	17.530419004144722
12	30.956145342033974	27.403944703917404	25.52912107760994	16.11078887643868
13	26.417355079424464	26.529039267792946	23.826879396703156	23.226726256079434
14	22.86616557387748	30.944930962625783	22.04333346508697	24.145569998409762
15	25.0792130447132	29.239793765724258	25.05673832532547	20.624254864237066
16	29.221087813186823	31.63489105965774	22.777185907753456	16.366835219401977
17	30.33756201230089	28.99381278788553	24.49059600749709	16.178029192316494
18	33.10296362956147	28.26331546692723	21.5105585220551	17.123162381456197
19	30.701615697925067	28.961088861087852	22.35793357594387	17.979361865043217
20	29.78061180874152	31.769417651984718	18.830735488639007	19.61923505063476
21	28.597724584033852	29.004429679866234	24.871563183376246	17.526282552723668
22	27.015945560622452	31.211364394713065	19.774214097210287	21.9984759474542
23	23.099783158024394	35.00311153068006	21.533998413441076	20.36310689785447
24	24.657157118051565	34.4451501945511	23.116007239709198	17.781685447688137
25	26.352918358398696	32.1455130073013	20.2708180705936	21.23075056370641
26	26.879948230012435	32.41755362909267	22.767442266628304	17.935055874266585
27	27.379493679961836	31.018008271064417	19.590831417543516	22.01166663143023
28	27.730770326752086	35.508356091476244	18.328708167836975	18.432165413934694
29	31.225014684402545	32.561272335688656	18.212703685762058	18.001009294146737
30	26.023289140711963	36.12156203436196	18.7777889104495	19.07735991447657
31	27.81322359174511	35.68291434144476	18.613709670747657	17.89015239606247
32	25.311957378004557	36.308253875165576	20.843119104982218	17.536669641847652
33	27.28417145499219	36.789047412006184	18.88823216339167	17.038548969609952
34	23.537097994372587	36.895262292384615	20.44303233142352	19.124607381819285
35	22.808347175125405	39.867670322983315	20.485821623345764	16.838160878545512
36	25.695774109307106	37.13237287995375	19.32678793516618	17.845065075572965
37	25.133998045756506	36.08654007899703	20.698251384102605	18.081210491143857
38	27.610399590399386	35.45945404356511	19.27324386954919	17.656902496486314
39	26.70401116290357	33.33253668343002	19.096755275584183	20.86669687808223
40	26.247898452875244	32.89434859622627	20.591117292297284	20.2666356586012
41	26.869515180317112	32.991003677764915	19.9320426992092	20.20743844270877
42	24.66690075917672	32.509566692925475	19.735561256709094	23.087971291188715
43	24.1833495880554	32.36934098975171	22.223085259617477	21.224224162575407
44	26.29339941850685	32.170975163826455	20.063122248685296	21.472503168981394
45	24.714883595660954	32.404179102831264	21.764904323878632	21.11603297762915
46	26.653408573852666	32.83119877119817	20.357223944722303	20.15816871022687
47	25.730428380101277	32.94862803098478	20.812187640467	20.508755948446947
48	26.473381015894088	31.649736324202195	20.943221229371748	20.933661430531973
49	26.297995475641358	31.48827683706702	21.442674758178455	20.771052929113175
50	24.111467254471734	32.114259818786664	22.299517689764308	21.474755236977302
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	48.0
8	96.0
9	166.0
10	236.0
11	1759.5
12	3283.0
13	10525.0
14	17767.0
15	12961.5
16	8156.0
17	8311.0
18	8466.0
19	8665.5
20	8865.0
21	9952.0
22	11039.0
23	13607.5
24	16176.0
25	18476.5
26	20777.0
27	25952.0
28	31127.0
29	37512.5
30	43898.0
31	53621.0
32	63344.0
33	62844.0
34	62344.0
35	61448.5
36	60553.0
37	66587.5
38	72622.0
39	83781.0
40	94940.0
41	100540.0
42	106140.0
43	92445.0
44	78750.0
45	75107.0
46	71464.0
47	75802.5
48	80141.0
49	86669.0
50	93197.0
51	90850.5
52	88504.0
53	94440.0
54	100376.0
55	103591.0
56	106806.0
57	112725.5
58	118645.0
59	135280.0
60	151915.0
61	146725.0
62	141535.0
63	136370.5
64	131206.0
65	129119.5
66	127033.0
67	110478.5
68	93924.0
69	77819.5
70	61715.0
71	47848.5
72	33982.0
73	30227.0
74	26472.0
75	21291.5
76	16111.0
77	15421.0
78	14731.0
79	10259.5
80	5788.0
81	4156.0
82	2524.0
83	1621.5
84	719.0
85	511.5
86	304.0
87	182.5
88	61.0
89	40.5
90	20.0
91	16.5
92	13.0
93	8.5
94	4.0
95	6.5
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2175778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.805497673858845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5857766761996	15.725997008134849
2	9.780277280317055	4.0696707241066195
3	3.6493388482819875	2.2777893275716083
4	1.7903565534263284	1.4899703643075763
5	1.0335133079622272	1.075137936235514
6	0.6910481311855723	0.8626560171543556
7	0.5217882300799027	0.7599244665022037
8	0.4035748091234583	0.671725980195691
9	0.32781971649196906	0.6138407114026856
>10	4.3029025444015545	23.11811990981498
>50	1.3241185672531424	18.80646408162526
>100	0.5552207971814936	19.138913339270967
>500	0.02051363793885583	2.97899229529795
>1k	0.012172928007672692	4.6668080884066825
>5k	6.762737782040384E-4	1.0452760880599747
>10k+	9.01698370938718E-4	2.6987136619131786
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA	20232	0.9298742794531427	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13590	0.6246041645792907	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGA	13055	0.6000152589096865	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA	10664	0.4901235328236613	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8442	0.3879991432949501	No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7823	0.3595495496323614	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6022	0.27677456063991823	No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA	4215	0.19372380821940474	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAA	3986	0.18319883738138726	No Hit
GCTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	3826	0.17584514596617853	Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3493	0.1605402757082754	No Hit
GCTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3460	0.1590235768538886	Illumina Single End Adapter 2 (100% over 34bp)
GCTGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	3212	0.14762535516031505	Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	2757	0.12671329519831526	No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2754	0.12657541348428009	No Hit
GCTGTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2716	0.12482891177316804	No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2553	0.11733733864392414	No Hit
GCTGTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2420	0.1112245826550319	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAA	2407	0.1106270952275462	No Hit
GCTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	2384	0.10957000208660994	Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC	2347	0.10786946094684291	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGG	2274	0.10451433923865394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.08300479184916844	0.0	0.0	0.0	0.0
8	0.10561739295093525	0.0	0.0	0.0	0.0
9	0.19399957164747506	0.0	0.0	0.0	0.0
10	0.4139668661049059	0.0	0.0	0.0	0.0
11	0.5316718893195905	0.0	0.0	0.0	0.0
12	0.6236849531523896	0.0	0.0	0.0	0.0
13	0.6832958141869253	0.0	0.0	0.0	0.0
14	0.7822029637214826	0.0	0.0	0.0	0.0
15	0.8738483429835213	0.0	0.0	0.0	0.0
16	0.9765702199397182	0.0	0.0	0.0	0.0
17	1.065090280350293	0.0	0.0	0.0	0.0
18	1.1491062047690528	0.0	0.0	0.0	0.0
19	1.2508629097270034	0.0	0.0	0.0	0.0
20	1.3630526643802814	0.0	0.0	0.0	0.0
21	1.4851239418727462	0.0	0.0	0.0	0.0
22	1.6294860964675624	0.0	0.0	0.0	0.0
23	1.775778595058871	0.0	0.0	0.0	0.0
24	1.9291490216373177	0.0	0.0	0.0	0.0
25	2.059998768256688	0.0	0.0	0.0	0.0
26	2.20932466455677	0.0	0.0	0.0	0.0
27	2.3250993437749625	0.0	0.0	0.0	0.0
28	2.510136604010152	0.0	0.0	0.0	0.0
29	2.605045183837689	0.0	0.0	0.0	0.0
30	2.7383768013096925	0.0	0.0	0.0	0.0
31	2.846889710255366	0.0	0.0	0.0	0.0
32	2.9604582820489957	0.0	0.0	0.0	0.0
33	3.0638695675753684	0.0	0.0	0.0	0.0
34	3.163649967965482	0.0	0.0	0.0	0.0
35	3.329016103664988	0.0	0.0	0.0	0.0
36	3.4189609417872595	0.0	0.0	0.0	0.0
37	3.5142831667569028	0.0	0.0	0.0	0.0
38	3.604090123165139	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTA	20	7.858032E-4	44.000004	38
ATACTCG	40	8.3182385E-9	44.000004	44
TACGTTC	20	7.858032E-4	44.000004	14
TACCGTA	20	7.858032E-4	44.000004	19
CGACGTA	30	2.5287627E-6	44.0	28
CGCGATT	25	4.444155E-5	44.0	13
TACGCTT	35	1.4468787E-7	44.0	23
CATACGC	35	1.4468787E-7	44.0	20
ATAACCG	25	4.444155E-5	44.0	29
TAATCGA	25	4.444155E-5	44.0	42
TGGGGAT	19520	0.0	39.345287	5
AAGCAGT	8465	0.0	38.880096	10
TGGGGGT	17040	0.0	38.758217	5
TGGGGAC	9180	0.0	38.607845	5
GTGGGGG	97795	0.0	38.510967	4
CGTCTAT	40	4.1267958E-7	38.500004	34
TGGGGGG	25520	0.0	38.474136	5
TGGGGAG	26380	0.0	37.80364	5
ACGTACG	35	7.293207E-6	37.714287	30
TATCCGA	35	7.293207E-6	37.714287	11
>>END_MODULE