FastQCFastQC Report
Fri 17 Jun 2016
SRR1527818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190379
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA265800.8331298569856433No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182060.5706532045252304No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAG173900.5450763059812016No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA133260.4176933210756465No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT118000.36986201325923973No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77850.24401489603586282No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC66970.20991236464382446No Hit
GCTGTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG53980.16919619894689628No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA52790.16546623457589207No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAA52490.1645259074235381No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42600.1335264556342679No Hit
GCTGTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT41940.1314577358990891No Hit
GCTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA38810.12164698927619572Illumina Single End Adapter 2 (100% over 34bp)
GCTGTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG37590.11782299218995611No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTC35830.11230640622947932No Hit
GCTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA34250.10735401656041493Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA33320.10443900238811753Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32910.10315388861323373No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGGAG32020.10036425139458352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG301.8624505E-646.046
ATTCGCG253.4185297E-546.010
TAACGAA253.4185297E-546.014
GTCCGAT206.313149E-446.044
TACGTTC700.042.71428714
TGGGGAT290850.041.532065
AAGCAGT137450.040.89632810
CGCATAC451.5899786E-840.8888938
CACGACG1350.040.8888934
TATCTCG402.9127477E-740.2535
GTGGGGG1443050.039.9975784
TGGGGAC141700.039.896975
TGGGGGG387350.039.8187685
GGAAGCA147950.039.8127758
TGGGGGT245500.039.7979665
ACGCGTA355.3761123E-639.42857423
GAAGCAG145250.039.3335659
TGGGGAG389100.038.9953775
CGTGTAT950.038.73684326
TGGGGGC418550.038.5100945
Produced by FastQC (version 0.11.5)