##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527818_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3190379
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84992441336907	31.0	31.0	34.0	30.0	34.0
2	32.334426097965164	34.0	31.0	34.0	31.0	34.0
3	32.31221400341464	34.0	31.0	34.0	30.0	34.0
4	36.15111997665481	37.0	35.0	37.0	35.0	37.0
5	34.84622892766032	35.0	35.0	37.0	33.0	37.0
6	35.827533656659604	37.0	35.0	37.0	35.0	37.0
7	36.495709443925	37.0	37.0	37.0	35.0	37.0
8	36.57408508518894	37.0	37.0	37.0	35.0	37.0
9	38.448447034035766	39.0	39.0	39.0	37.0	39.0
10	37.58028152768057	39.0	37.0	39.0	35.0	39.0
11	36.894365528358854	39.0	35.0	39.0	33.0	39.0
12	36.802951937685144	39.0	35.0	39.0	33.0	39.0
13	36.64311951652139	39.0	35.0	39.0	33.0	39.0
14	37.55866654087179	40.0	36.0	40.0	33.0	40.0
15	37.700762824730226	40.0	37.0	40.0	33.0	40.0
16	37.61323184486859	40.0	36.0	40.0	33.0	40.0
17	37.54056712384328	40.0	36.0	40.0	33.0	40.0
18	37.59972310499787	40.0	36.0	40.0	33.0	40.0
19	37.439589152260595	40.0	36.0	40.0	33.0	40.0
20	37.616990019054164	40.0	37.0	40.0	33.0	40.0
21	37.59153160173133	40.0	36.0	40.0	33.0	40.0
22	37.46813027543123	40.0	36.0	40.0	33.0	40.0
23	37.28583469236727	39.0	36.0	40.0	33.0	40.0
24	37.012846122670695	39.0	35.0	40.0	33.0	40.0
25	36.66838642054753	38.0	35.0	40.0	32.0	40.0
26	36.36917244001418	38.0	35.0	40.0	31.0	40.0
27	36.31063111937485	38.0	35.0	40.0	31.0	40.0
28	36.186139013578014	38.0	35.0	40.0	31.0	40.0
29	36.00840777851158	38.0	35.0	40.0	30.0	40.0
30	35.647436871920235	38.0	35.0	40.0	30.0	40.0
31	35.38127633111928	38.0	35.0	40.0	28.0	40.0
32	34.911694817449586	37.0	34.0	40.0	26.0	40.0
33	34.75546917779988	37.0	34.0	40.0	25.0	40.0
34	34.319165842051994	37.0	33.0	40.0	22.0	40.0
35	34.0898507669465	37.0	33.0	40.0	21.0	40.0
36	33.810626261017894	37.0	33.0	40.0	20.0	40.0
37	33.2975235857558	37.0	33.0	40.0	15.0	40.0
38	33.04363243363876	36.0	33.0	40.0	15.0	40.0
39	32.668768820256155	36.0	32.0	40.0	11.0	40.0
40	32.2012055620978	35.0	31.0	40.0	10.0	40.0
41	31.952006642471005	35.0	31.0	39.0	10.0	40.0
42	31.71672895289243	35.0	31.0	39.0	10.0	40.0
43	31.491851281618892	35.0	30.0	39.0	10.0	40.0
44	31.358589998241587	35.0	30.0	39.0	10.0	40.0
45	31.151209934619054	35.0	30.0	39.0	9.0	40.0
46	30.93305748313915	35.0	29.0	39.0	9.0	40.0
47	30.74547318672797	35.0	28.0	38.0	9.0	40.0
48	30.516313265602612	35.0	28.0	38.0	9.0	40.0
49	30.569635143661614	35.0	28.0	39.0	9.0	40.0
50	30.311868589907345	35.0	27.0	39.0	8.0	40.0
51	30.217393920910336	35.0	27.0	38.0	8.0	40.0
52	29.692648114847795	35.0	25.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	14.0
14	27.0
15	57.0
16	215.0
17	568.0
18	1400.0
19	3272.0
20	6514.0
21	11692.0
22	19930.0
23	32253.0
24	45642.0
25	61613.0
26	81858.0
27	101486.0
28	105094.0
29	96270.0
30	88620.0
31	95527.0
32	118654.0
33	160017.0
34	270334.0
35	245758.0
36	319079.0
37	458001.0
38	642082.0
39	224394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.0190507146643	0.6096140928710978	0.2676171075599482	0.10371808490464614
8	98.29653467503391	0.954463403877721	0.4430194657123809	0.3059824553759914
9	92.29991170328039	4.278770641356403	1.9541565437836694	1.4671611115795333
10	42.333810497122755	43.84670285254511	6.489511120779068	7.329975529553072
11	25.95776238497056	36.874678525654794	19.592186382871756	17.575372706502897
12	31.263307588220712	26.753498565530933	25.578998608002372	16.40419523824599
13	26.331009575978275	25.943814198877313	23.8208689312461	23.90430729389831
14	22.78961214325947	30.772174716546214	22.22109034694624	24.217122793248073
15	25.594702071446683	28.55137273659337	25.151964703880008	20.701960488079944
16	29.22890979410283	30.821761301713686	23.354059188579164	16.59526971560432
17	30.715849120120208	28.335536310889708	24.490287831006913	16.458326737983167
18	33.50272804579017	27.56741440437014	21.577279689967867	17.352577859871822
19	30.548188788855494	28.40170399817702	22.88242243319681	18.167684779770678
20	29.694089636372357	31.62260659313517	18.976804950132884	19.706498820359588
21	28.623401796463682	28.526892886393746	25.212803870637313	17.63690144650526
22	26.97795465679783	30.603762123559612	19.93380723732196	22.484475982320596
23	23.19555137493069	34.79621073232992	21.601602818975426	20.40663507376396
24	24.71490064346587	34.16484373800103	23.049330502739643	18.07092511579345
25	26.319725650150026	31.441029420015614	20.25295427283091	21.986290657003448
26	27.22353676475428	31.733032345059943	22.846658657168945	18.19677223301683
27	27.27472190608075	30.2573456006324	19.738783385923742	22.729149107363106
28	27.55086464648871	35.24289747393648	18.50661002971747	18.699627849857336
29	31.41570327537888	31.926865115398517	18.403800927726767	18.253630681495835
30	26.017880634244396	35.751457742167936	18.92605862814418	19.30460299544349
31	28.34531571327419	34.81962487842353	18.72125537436148	18.113804033940795
32	25.768599906155348	35.468137171163676	21.05505333378887	17.708209588892103
33	27.947337918159565	35.894356125087334	18.92433469503153	17.23397126172157
34	23.633210975874654	36.079193099001714	20.635134571785983	19.652461353337642
35	22.866875690944553	39.49267469476197	20.65159029695218	16.988859317341294
36	26.12159871914904	36.505600118355844	19.36961094590956	18.00319021658555
37	25.23051336533998	35.5383169209677	20.987067680673675	18.244102033018645
38	27.806476910736937	34.993804811277904	19.349707354518067	17.850010923467085
39	26.62972016804273	32.869919216494345	19.17054368775622	21.32981692770671
40	26.21453438604003	32.43993895396127	20.835549632191032	20.50997702780767
41	27.034499662892713	32.44683468641186	20.095104688189082	20.423560962506336
42	24.736810266115718	31.891477470231592	19.820121684602363	23.551590579050327
43	24.27131698146208	31.76190038863721	22.5179516289444	21.44883100095631
44	26.618686996121777	31.41730183153788	20.22596688355835	21.738044288781992
45	24.962802225064795	31.625584295784293	22.07546501528502	21.336148463865893
46	26.990931171500314	32.213132044813484	20.421492242771155	20.374444540915047
47	26.271894342333624	32.254945258854825	20.86817898437772	20.604981414433833
48	27.077002450179116	30.964032799864842	20.935192966102147	21.023771783853892
49	26.71030620499947	30.8568668487349	21.567813729967504	20.865013216298127
50	23.96624350900003	31.864552769435857	22.318257486022823	21.850946235541294
51	22.840797284585936	31.53898016505249	23.181227057976496	22.438995492385075
52	25.423562529718257	30.058936571485706	22.578351976363937	21.9391489224321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	58.0
8	116.0
9	222.0
10	328.0
11	2270.0
12	4212.0
13	14134.5
14	17343.5
15	10630.0
16	11004.5
17	11379.0
18	11320.5
19	11262.0
20	11916.0
21	12570.0
22	14936.5
23	17303.0
24	18646.0
25	19989.0
26	25762.5
27	31536.0
28	40724.0
29	49912.0
30	59487.5
31	69063.0
32	73198.0
33	77333.0
34	81419.5
35	85506.0
36	94714.5
37	103923.0
38	118790.5
39	140205.0
40	146752.0
41	127428.5
42	108105.0
43	105210.5
44	102316.0
45	106761.5
46	111207.0
47	120128.5
48	129050.0
49	122171.0
50	115292.0
51	121861.5
52	128431.0
53	136481.0
54	144531.0
55	149583.5
56	154636.0
57	162106.0
58	169576.0
59	187645.5
60	205715.0
61	209114.5
62	212514.0
63	200563.0
64	186591.0
65	184570.0
66	167740.5
67	150911.0
68	125198.0
69	99485.0
70	80638.5
71	61792.0
72	51361.5
73	40931.0
74	34601.0
75	28271.0
76	27116.5
77	25962.0
78	18966.5
79	11971.0
80	8245.5
81	4520.0
82	2999.5
83	1479.0
84	1092.0
85	705.0
86	456.5
87	208.0
88	119.5
89	24.0
90	17.0
91	10.5
92	4.0
93	4.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3190379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.45430080382738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61791310377347	16.866923562975185
2	8.799065576792536	3.7755559935421985
3	3.4854431110118362	2.2433323481482756
4	1.787113464369929	1.5336507934065
5	0.9967468837282077	1.0692253734391262
6	0.6489046993029174	0.8353077967117168
7	0.440027938179665	0.6608344223456144
8	0.32058003910509847	0.5502256472530821
9	0.25110762717273866	0.4848604710749347
>10	2.601103657650433	13.561362348882215
>50	1.2710491631769547	19.434071278457548
>100	0.7462760663624443	27.454706900310683
>500	0.020713060939367783	3.0269500396165885
>1k	0.012486597020186252	4.793172535479413
>5k	7.345057070697796E-4	0.9583571609035663
>10k+	7.345057070697796E-4	2.751463327453405
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA	26580	0.8331298569856433	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18206	0.5706532045252304	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAG	17390	0.5450763059812016	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA	13326	0.4176933210756465	No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11800	0.36986201325923973	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7785	0.24401489603586282	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6697	0.20991236464382446	No Hit
GCTGTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5398	0.16919619894689628	No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA	5279	0.16546623457589207	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	5249	0.1645259074235381	No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4260	0.1335264556342679	No Hit
GCTGTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4194	0.1314577358990891	No Hit
GCTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	3881	0.12164698927619572	Illumina Single End Adapter 2 (100% over 34bp)
GCTGTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3759	0.11782299218995611	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTC	3583	0.11230640622947932	No Hit
GCTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	3425	0.10735401656041493	Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3332	0.10443900238811753	Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3291	0.10315388861323373	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGGAG	3202	0.10036425139458352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.07171561748619835	0.0	0.0	0.0	0.0
8	0.09353120742081113	0.0	0.0	0.0	0.0
9	0.17740838941078788	0.0	0.0	0.0	0.0
10	0.3790145308754853	0.0	0.0	0.0	0.0
11	0.49097615048243487	0.0	0.0	0.0	0.0
12	0.574163759227352	0.0	0.0	0.0	0.0
13	0.631210273136828	0.0	0.0	0.0	0.0
14	0.7211368931402821	0.0	0.0	0.0	0.0
15	0.8033528304944334	0.0	0.0	0.0	0.0
16	0.8955989241403608	0.0	0.0	0.0	0.0
17	0.9748685030838029	0.0	0.0	0.0	0.0
18	1.0491856923581806	0.0	0.0	0.0	0.0
19	1.1427482440174035	0.0	0.0	0.0	0.0
20	1.2370003689216862	0.0	0.0	0.0	0.0
21	1.340436355680626	0.0	0.0	0.0	0.0
22	1.4608922638971733	0.0	0.0	0.0	0.0
23	1.581943712643545	0.0	0.0	0.0	0.0
24	1.7078221741053337	0.0	0.0	0.0	0.0
25	1.8156776984803373	0.0	0.0	0.0	0.0
26	1.9400202922599479	0.0	0.0	0.0	0.0
27	2.028317011865988	0.0	0.0	0.0	0.0
28	2.177734996375039	0.0	0.0	0.0	3.1344238411799976E-5
29	2.2451877974372323	0.0	0.0	0.0	3.1344238411799976E-5
30	2.3521970273751176	0.0	0.0	0.0	3.1344238411799976E-5
31	2.4393340101599215	0.0	0.0	0.0	3.1344238411799976E-5
32	2.5342757083092637	0.0	0.0	0.0	3.1344238411799976E-5
33	2.6198141349350657	0.0	0.0	0.0	3.1344238411799976E-5
34	2.7081108545411063	0.0	0.0	0.0	3.1344238411799976E-5
35	2.857152708189215	0.0	0.0	0.0	3.1344238411799976E-5
36	2.935231206073009	0.0	0.0	0.0	3.1344238411799976E-5
37	3.0174157991887482	0.0	0.0	0.0	3.1344238411799976E-5
38	3.0937390197214816	0.0	0.0	0.0	3.1344238411799976E-5
39	3.1678994878038003	0.0	0.0	0.0	3.1344238411799976E-5
40	3.2469183128399477	0.0	0.0	0.0	3.1344238411799976E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	1.8624505E-6	46.0	46
ATTCGCG	25	3.4185297E-5	46.0	10
TAACGAA	25	3.4185297E-5	46.0	14
GTCCGAT	20	6.313149E-4	46.0	44
TACGTTC	70	0.0	42.714287	14
TGGGGAT	29085	0.0	41.53206	5
AAGCAGT	13745	0.0	40.896328	10
CGCATAC	45	1.5899786E-8	40.88889	38
CACGACG	135	0.0	40.88889	34
TATCTCG	40	2.9127477E-7	40.25	35
GTGGGGG	144305	0.0	39.997578	4
TGGGGAC	14170	0.0	39.89697	5
TGGGGGG	38735	0.0	39.818768	5
GGAAGCA	14795	0.0	39.812775	8
TGGGGGT	24550	0.0	39.797966	5
ACGCGTA	35	5.3761123E-6	39.428574	23
GAAGCAG	14525	0.0	39.333565	9
TGGGGAG	38910	0.0	38.995377	5
CGTGTAT	95	0.0	38.736843	26
TGGGGGC	41855	0.0	38.510094	5
>>END_MODULE