FastQCFastQC Report
Fri 17 Jun 2016
SRR1527816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594076
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG130582.1980352682148414No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC98691.661235262828325No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT36860.6204593351692376No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT29300.4932028898659431No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT25800.43428786889219567No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA21610.3637581723550522No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC20000.33665726270712837No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGT13690.23044189632302936No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC13150.22135215022993693No Hit
TCGGGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT12710.2139456904503801No Hit
TCGGGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA12200.2053609302513483No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTG10590.1782600206034245No Hit
TCGGGTGGGGCTCCCACGGTGACTTGTTTACAGCTGGGTGTGACTCAGTA10510.17691339155259597No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTC9980.16799197409085706No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCG9380.1578922562096432No Hit
TCGGGTGGGGCCCGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGT9160.1541890263198648No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT8930.15031746779873284No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGGTAAGAGCACTGACTGCT8470.14257435075646888No Hit
TCGGGTGGGCGAGCAAGATGTTGGACACGTGTACAATCCTGTATCATTTA8450.14223769349376175No Hit
TCGGGTGGGAGCCGGGCGTGGTGGCCCACACCTTTAATCTCATCATCTCA8360.14072273581157965No Hit
TCGGGTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG8320.1400494212861654No Hit
TCGGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG7970.13415791918879066No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG7630.12843474572276947No Hit
TCGGGTGGGCCAGAGTGTGGGACTCACTCTGAGTCAAATAAAACTGCTAC7310.12304822951945543No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCTGTTAAGAGCACTGACTGC7270.12237491499404117No Hit
TCGGGTGGGGGAGAGATGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT7200.12119661457456621No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC6890.11597842700260573No Hit
TCGGGTGGGGGGCCAGGCAGTGGTGGTGCACGCCATTAATCCCAGCACTT6870.1156417697398986No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGC6840.1151367838458379No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC6650.1119385398501202No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT6530.10991859627387743No Hit
TCGGGTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT6510.1095819390111703No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA6500.10941361037981673No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGC6350.10688868090951327No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG5950.1001555356553707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCT207.853406E-444.026
TCGCATA207.853406E-444.021
TCCGCGC351.4444959E-744.030
CGTATGA207.853406E-444.024
GTACACC207.853406E-444.042
CGAAAAT207.853406E-444.018
CGACGAG502.7284841E-1144.038
CTATCGC207.853406E-444.018
GCGCGCA302.5256431E-644.014
CTTTTCG454.783942E-1044.011
GTTTACG454.783942E-1044.039
GACGTAG254.4402354E-544.010
GGCGTAT254.4402354E-544.022
TGGCGAT302.5256431E-644.019
CGACCTG207.853406E-444.034
CCGGACT302.5256431E-644.042
CGCGCAT254.4402354E-544.015
GCCGGAT408.300049E-944.027
CGCGCAC207.853406E-444.022
ATTCGGT302.5256431E-644.018
Produced by FastQC (version 0.11.5)