##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527816_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594076
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46753950672978	31.0	31.0	33.0	31.0	33.0
2	32.27020953548031	31.0	31.0	34.0	31.0	34.0
3	32.93744571401639	34.0	31.0	34.0	31.0	34.0
4	36.46902921511726	37.0	37.0	37.0	35.0	37.0
5	36.481036096391705	37.0	37.0	37.0	35.0	37.0
6	31.814227472579265	33.0	32.0	35.0	28.0	35.0
7	34.728021330604165	35.0	35.0	35.0	32.0	35.0
8	36.086480517644205	37.0	35.0	37.0	35.0	37.0
9	38.68061325486975	39.0	39.0	39.0	39.0	39.0
10	38.15622411947293	39.0	39.0	39.0	37.0	39.0
11	37.737646361744964	39.0	37.0	39.0	35.0	39.0
12	37.063574357489614	39.0	35.0	39.0	34.0	39.0
13	36.951778896976144	39.0	35.0	39.0	34.0	39.0
14	37.534184178455284	39.0	36.0	40.0	33.0	40.0
15	37.547628586241494	39.0	36.0	40.0	33.0	40.0
16	37.56459779556825	39.0	36.0	40.0	33.0	40.0
17	37.258083140877595	39.0	36.0	40.0	33.0	40.0
18	37.0751503174678	39.0	35.0	40.0	32.0	40.0
19	37.12368451174597	39.0	35.0	40.0	32.0	40.0
20	36.94554063789818	39.0	35.0	40.0	31.0	40.0
21	36.952997596267146	39.0	35.0	40.0	32.0	40.0
22	37.12619260835314	39.0	35.0	40.0	32.0	40.0
23	37.212871753782345	39.0	35.0	40.0	33.0	40.0
24	37.211333230091775	39.0	35.0	40.0	33.0	40.0
25	37.24009722661747	39.0	35.0	40.0	33.0	40.0
26	37.07073169089477	38.0	35.0	40.0	33.0	40.0
27	36.98010018920138	38.0	35.0	40.0	33.0	40.0
28	36.87181943051058	38.0	35.0	40.0	33.0	40.0
29	37.15967317312936	39.0	35.0	40.0	33.0	40.0
30	37.3512042230287	39.0	35.0	40.0	33.0	40.0
31	37.44675260404393	40.0	35.0	40.0	34.0	40.0
32	37.43000558851056	40.0	35.0	40.0	34.0	40.0
33	37.37361213043449	40.0	35.0	40.0	33.0	40.0
34	36.866496879187174	39.0	35.0	40.0	32.0	40.0
35	36.849138830722	39.0	35.0	40.0	32.0	40.0
36	37.018947070745156	39.0	35.0	40.0	33.0	40.0
37	37.08860482497189	39.0	35.0	40.0	33.0	40.0
38	37.01104572478942	39.0	35.0	40.0	33.0	40.0
39	36.92517792336334	39.0	35.0	40.0	33.0	40.0
40	36.82065762629697	39.0	35.0	40.0	33.0	40.0
41	36.704844161353094	39.0	35.0	40.0	32.0	40.0
42	36.623152593270895	39.0	35.0	40.0	32.0	40.0
43	36.55816932513685	39.0	35.0	40.0	32.0	40.0
44	36.3344891899353	38.0	35.0	40.0	32.0	40.0
45	36.23464674553424	38.0	35.0	40.0	32.0	40.0
46	36.01118712083976	38.0	35.0	40.0	31.0	40.0
47	35.97540382038662	37.0	35.0	40.0	31.0	40.0
48	35.94318403705923	37.0	35.0	40.0	31.0	40.0
49	35.83243558063278	37.0	35.0	40.0	31.0	40.0
50	35.20006026165002	36.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	1.0
15	6.0
16	20.0
17	28.0
18	88.0
19	166.0
20	248.0
21	340.0
22	519.0
23	810.0
24	1217.0
25	1656.0
26	2277.0
27	3085.0
28	4279.0
29	6106.0
30	8686.0
31	11400.0
32	16601.0
33	26611.0
34	65902.0
35	40200.0
36	56148.0
37	104222.0
38	222131.0
39	21326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.26070065109515	0.4987577347006107	0.15503066947663263	0.0855109447276106
8	98.77103266248763	0.8992115486907398	0.1856664803829813	0.14408930843865095
9	96.77852665315548	1.6772264828069137	0.7714501174933848	0.7727967465442133
10	59.26295625475528	27.551525394057325	5.251684969599849	7.93383338158754
11	35.17681239437378	20.61823739723537	23.305603996795025	20.899346211595823
12	32.249072509241245	16.028588934749088	28.61670897326268	23.10562958274699
13	27.106296164127148	16.45681697291256	28.904550932877278	27.53233593008302
14	22.80196473178516	19.133073882802872	34.11112382927437	23.9538375561376
15	26.520175869754038	21.032494158996492	31.29128259683946	21.156047374410008
16	32.85051070906753	21.35450683077586	26.916589796591683	18.87839266356493
17	31.823032743285374	21.324880991657633	25.26494926575051	21.587136999306487
18	29.344225317972782	23.393976528255646	26.737488132831487	20.52431002094008
19	29.465085275284643	25.487479716399925	24.397046842491534	20.6503881658239
20	29.868064018745077	24.918528942424874	25.134831233714205	20.078575805115843
21	29.020192702617177	24.7803311360836	26.36430355712064	19.835172604178588
22	25.81504723301396	23.223964610588542	30.509059446939453	20.451928709458052
23	29.65007844114221	21.3162962314586	29.029955763235684	20.003669564163506
24	32.1122213319508	20.939576754489327	25.277068927207967	21.671132986351914
25	28.568398656064208	22.100539324934857	23.16471293235209	26.166349086648843
26	24.336111877941544	22.914913243423403	26.828048936499705	25.920925942135348
27	22.07377507254964	22.429958456493782	26.995704253327858	28.500562217628723
28	22.330812892626533	25.28481204425023	24.33594354931019	28.04843151381305
29	28.984675361401575	24.80608541668069	22.589702327648315	23.61953689426942
30	27.231700994485553	21.894505080158094	27.14753667880877	23.72625724654758
31	29.378564358768912	20.590968158956095	25.820938735111334	24.209528747163663
32	32.988876844040156	22.3646469475286	24.74885368202048	19.897622526410764
33	33.804092405685466	20.659141254654287	25.727684673341457	19.809081666318786
34	27.041489641056028	23.748981611780312	29.861835859384993	19.347692887778667
35	23.322436859930377	29.375871100667254	29.111931806704867	18.1897602326975
36	25.495727819336246	32.55206404567766	23.400036358984373	18.552171776001725
37	28.225513233996995	29.273527292804285	23.11051111305624	19.39044836014247
38	27.374275345242022	28.984843690032925	21.974461180051037	21.666419784674016
39	27.552703694476797	27.246850571307373	22.68059978857924	22.519845945636586
40	27.079195254479227	24.680175600428228	22.894377150398267	25.34625199469428
41	22.09835105272726	26.894875403147072	22.517826002060342	28.488947542065326
42	21.96318316175035	26.976178132090844	23.44195018819141	27.6186885179674
43	21.352318558568264	23.406769504238515	23.71868245813667	31.522229479056552
44	23.052437735239263	22.361280374901526	25.86234757842431	28.723934311434903
45	25.929679030965737	23.758239686504755	24.927955345780674	25.384125936748838
46	25.575852247860542	26.296803775947858	23.198546987254158	24.928796988937442
47	21.778358324524135	26.20338138554663	26.21516438974138	25.803095900187856
48	25.105542051858688	22.94049919538914	27.79728519583353	24.156673556918644
49	26.36447188575199	22.435681629959802	27.3424612339162	23.85738525037201
50	24.24622438879874	23.03257495673954	27.442785098202922	25.278415556258793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	2.0
10	1.0
11	3.0
12	5.0
13	81.0
14	157.0
15	431.0
16	705.0
17	909.5
18	1114.0
19	947.5
20	781.0
21	1029.0
22	1277.0
23	1358.0
24	1439.0
25	1856.0
26	2273.0
27	2773.5
28	3274.0
29	3652.0
30	4030.0
31	5213.0
32	6396.0
33	6100.0
34	5804.0
35	8874.0
36	11944.0
37	11379.0
38	10814.0
39	13047.5
40	15281.0
41	15862.0
42	16443.0
43	18061.5
44	19680.0
45	20358.5
46	21037.0
47	22655.5
48	24274.0
49	26272.5
50	28271.0
51	32784.0
52	37297.0
53	34413.5
54	31530.0
55	34742.0
56	37954.0
57	38555.0
58	39156.0
59	44154.0
60	49152.0
61	51115.5
62	53079.0
63	53121.5
64	53164.0
65	56967.5
66	60771.0
67	43078.5
68	25386.0
69	19728.0
70	14070.0
71	10521.0
72	6972.0
73	6055.0
74	5138.0
75	4258.0
76	3378.0
77	2268.5
78	1159.0
79	846.5
80	534.0
81	389.0
82	244.0
83	158.0
84	72.0
85	40.5
86	9.0
87	6.0
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	594076.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.878661989375097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.5396428165169	5.9078299746160425
2	9.340992681909528	1.6587103333580215
3	3.4504986160087974	0.9190743271904604
4	1.611496606377735	0.5723173466021182
5	0.9896485041519736	0.4393377278328025
6	0.6711409395973155	0.35753001299497034
7	0.4834489819133205	0.30046660696611205
8	0.4057179691351003	0.2881786168773019
9	0.312819929473325	0.2499680175600428
>10	10.459560914571721	26.447626229640655
>50	3.8088196261327876	22.928716191194393
>100	1.833314374549729	28.496185673213525
>500	0.06825162097599818	4.100148802510117
>1k	0.020854661964888332	3.474639608400272
>5k	0.001895878360444394	1.661235262828325
>10k+	0.001895878360444394	2.1980352682148414
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	13058	2.1980352682148414	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9869	1.661235262828325	No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3686	0.6204593351692376	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2930	0.4932028898659431	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2580	0.43428786889219567	No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	2161	0.3637581723550522	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	2000	0.33665726270712837	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGT	1369	0.23044189632302936	No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1315	0.22135215022993693	No Hit
TCGGGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	1271	0.2139456904503801	No Hit
TCGGGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA	1220	0.2053609302513483	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTG	1059	0.1782600206034245	No Hit
TCGGGTGGGGCTCCCACGGTGACTTGTTTACAGCTGGGTGTGACTCAGTA	1051	0.17691339155259597	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTC	998	0.16799197409085706	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCG	938	0.1578922562096432	No Hit
TCGGGTGGGGCCCGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGT	916	0.1541890263198648	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	893	0.15031746779873284	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGGTAAGAGCACTGACTGCT	847	0.14257435075646888	No Hit
TCGGGTGGGCGAGCAAGATGTTGGACACGTGTACAATCCTGTATCATTTA	845	0.14223769349376175	No Hit
TCGGGTGGGAGCCGGGCGTGGTGGCCCACACCTTTAATCTCATCATCTCA	836	0.14072273581157965	No Hit
TCGGGTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG	832	0.1400494212861654	No Hit
TCGGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG	797	0.13415791918879066	No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	763	0.12843474572276947	No Hit
TCGGGTGGGCCAGAGTGTGGGACTCACTCTGAGTCAAATAAAACTGCTAC	731	0.12304822951945543	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCTGTTAAGAGCACTGACTGC	727	0.12237491499404117	No Hit
TCGGGTGGGGGAGAGATGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	720	0.12119661457456621	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	689	0.11597842700260573	No Hit
TCGGGTGGGGGGCCAGGCAGTGGTGGTGCACGCCATTAATCCCAGCACTT	687	0.1156417697398986	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGC	684	0.1151367838458379	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	665	0.1119385398501202	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	653	0.10991859627387743	No Hit
TCGGGTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT	651	0.1095819390111703	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	650	0.10941361037981673	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGC	635	0.10688868090951327	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG	595	0.1001555356553707	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.002524929470303463	0.0	0.0	0.0	0.0
8	0.003534901258424848	0.0	0.0	0.0	0.0
9	0.005049858940606926	0.0	0.0	0.0	0.0
10	0.013971276402345828	0.0	0.0	0.0	0.0
11	0.032655754482591455	0.0	0.0	0.0	0.0
12	0.043933772783280256	0.0	0.0	0.0	0.0
13	0.05117190393148351	0.0	0.0	0.0	0.0
14	0.059251678236454594	0.0	0.0	0.0	0.0
15	0.07019303927443626	0.0	0.0	0.0	0.0
16	0.08046108578700369	0.0	0.0	0.0	0.0
17	0.09039247503686397	0.0	0.0	0.0	0.0
18	0.10251213649432059	0.0	0.0	0.0	0.0
19	0.1151367838458379	0.0	0.0	0.0	0.0
20	0.12557315898975888	0.0	0.0	0.0	0.0
21	0.13331627603202284	0.0	0.0	0.0	0.0
22	0.14156437896834748	0.0	0.0	0.0	0.0
23	0.15166409684956134	0.0	0.0	0.0	0.0
24	0.16041718567994667	0.0	0.0	0.0	0.0
25	0.16900194587897846	0.0	0.0	0.0	0.0
26	0.17994330691696012	0.0	0.0	0.0	0.0
27	0.1875180953278705	0.0	0.0	0.0	0.0
28	0.20014274267938784	0.0	0.0	0.0	0.0
29	0.2087275028784196	0.0	0.0	0.0	0.0
30	0.22067883570452265	0.0	0.0	0.0	0.0
31	0.23111521084844364	0.0	0.0	0.0	0.0
32	0.24272988641183957	0.0	0.0	0.0	0.0
33	0.25232461839899273	0.0	0.0	0.0	0.0
34	0.26360263669968154	0.0	0.0	0.0	0.0
35	0.27807889899608806	0.0	0.0	0.0	0.0
36	0.2937334617119695	0.0	0.0	0.0	0.0
37	0.30703142358890106	0.0	0.0	0.0	0.0
38	0.3196560709404184	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCT	20	7.853406E-4	44.0	26
TCGCATA	20	7.853406E-4	44.0	21
TCCGCGC	35	1.4444959E-7	44.0	30
CGTATGA	20	7.853406E-4	44.0	24
GTACACC	20	7.853406E-4	44.0	42
CGAAAAT	20	7.853406E-4	44.0	18
CGACGAG	50	2.7284841E-11	44.0	38
CTATCGC	20	7.853406E-4	44.0	18
GCGCGCA	30	2.5256431E-6	44.0	14
CTTTTCG	45	4.783942E-10	44.0	11
GTTTACG	45	4.783942E-10	44.0	39
GACGTAG	25	4.4402354E-5	44.0	10
GGCGTAT	25	4.4402354E-5	44.0	22
TGGCGAT	30	2.5256431E-6	44.0	19
CGACCTG	20	7.853406E-4	44.0	34
CCGGACT	30	2.5256431E-6	44.0	42
CGCGCAT	25	4.4402354E-5	44.0	15
GCCGGAT	40	8.300049E-9	44.0	27
CGCGCAC	20	7.853406E-4	44.0	22
ATTCGGT	30	2.5256431E-6	44.0	18
>>END_MODULE