SRR1527815_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	903415
Sequences flagged as poor quality	0
Sequence length	52
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	18574	2.055976489210385	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14560	1.6116624142835794	No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5625	0.6226374368368912	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4623	0.5117249547550129	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3722	0.41199227376122827	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3204	0.35465428402229315	No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2977	0.3295274043490533	No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2131	0.2358827338487849	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTC	2124	0.23510789614961008	No Hit
TCGGGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	1817	0.20112572848580113	No Hit
TCGGGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	1756	0.19437357139299216	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCT	1523	0.1685825451204596	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC	1519	0.16813978072093114	No Hit
TCGGGTGGGGCTCCCACGGTGACTTGTTTACAGCTGGGTGTGACTCAGTAAA	1472	0.16293729902647178	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCGCT	1460	0.1616090058278864	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	1430	0.158288272831423	No Hit
TCGGGTGGGGCCCGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGTAT	1400	0.15496753983495956	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGGTAAGAGCACTGACTGCTCT	1294	0.1432342832474555	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	1272	0.140799079050049	No Hit
TCGGGTGGGAGCCGGGCGTGGTGGCCCACACCTTTAATCTCATCATCTCATC	1252	0.13858525705240668	No Hit
TCGGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	1251	0.13847456595252458	No Hit
TCGGGTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1247	0.13803180155299613	No Hit
TCGGGTGGGCGAGCAAGATGTTGGACACGTGTACAATCCTGTATCATTTATG	1221	0.13515383295606118	No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1117	0.12364195856832132	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCTGTTAAGAGCACTGACTGCTC	1069	0.11832878577397983	No Hit
TCGGGTGGGCCAGAGTGTGGGACTCACTCTGAGTCAAATAAAACTGCTACTG	1052	0.1164470370759839	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC	1030	0.11401183287857741	No Hit
TCGGGTGGGGGAGAGATGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTC	1025	0.11345837737916684	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	1024	0.11334768627928471	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1016	0.1124621574802278	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC	1006	0.11135524648140667	No Hit
TCGGGTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	999	0.11058040878223185	No Hit
TCGGGTGGGGGGCCAGGCAGTGGTGGTGCACGCCATTAATCCCAGCACTTGG	990	0.10958418888329284	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	909	0.1006182097928416	No Hit
TCGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	906	0.10028613649319526	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	40	5.6097633E-9	46.000004	9
CCGGGTC	20	6.3107326E-4	46.000004	34
CGAACAA	20	6.3107326E-4	46.000004	27
CGAAAGG	40	5.6097633E-9	46.000004	36
CGCGGAC	35	1.0192525E-7	46.000004	38
CGACGAA	20	6.3107326E-4	46.000004	24
ACGTTAA	40	5.6097633E-9	46.000004	12
GGCACGA	20	6.3107326E-4	46.000004	9
CGGTTAG	20	6.3107326E-4	46.000004	29
CGTGAAA	20	6.3107326E-4	46.000004	34
ACCGACC	20	6.3107326E-4	46.000004	46
CGACCGA	40	5.6097633E-9	46.000004	13
CGCGCAC	35	1.0192525E-7	46.000004	22
CCTACCG	20	6.3107326E-4	46.000004	27
ATTCGGT	80	0.0	46.000004	18
TTCGTCG	20	6.3107326E-4	46.000004	17
CGTCTAC	20	6.3107326E-4	46.000004	27
CGATTGT	20	6.3107326E-4	46.000004	14
CGATTAC	20	6.3107326E-4	46.000004	25
TCGATTG	20	6.3107326E-4	46.000004	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content