##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	903415
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54527653404028	31.0	31.0	33.0	30.0	33.0
2	32.087645212886656	31.0	31.0	34.0	31.0	34.0
3	32.71750856472385	34.0	31.0	34.0	31.0	34.0
4	36.39331204374512	37.0	37.0	37.0	35.0	37.0
5	36.34637791048411	37.0	37.0	37.0	35.0	37.0
6	31.626591322924682	33.0	32.0	35.0	28.0	35.0
7	34.75128927458588	35.0	35.0	35.0	32.0	35.0
8	36.09276135552321	37.0	35.0	37.0	35.0	37.0
9	38.65064007128507	39.0	39.0	39.0	38.0	39.0
10	38.04134201889497	39.0	38.0	39.0	35.0	39.0
11	37.65003791170171	39.0	37.0	39.0	35.0	39.0
12	36.972108056651706	39.0	35.0	39.0	34.0	39.0
13	36.89281338034015	39.0	35.0	39.0	33.0	39.0
14	37.56196100352551	40.0	36.0	40.0	33.0	40.0
15	37.610302020666026	40.0	36.0	40.0	33.0	40.0
16	37.65411687873237	40.0	36.0	40.0	33.0	40.0
17	37.433790672061015	40.0	36.0	40.0	33.0	40.0
18	37.37781528976163	40.0	36.0	40.0	33.0	40.0
19	37.44042217585495	40.0	36.0	40.0	33.0	40.0
20	37.40108809351184	40.0	35.0	40.0	33.0	40.0
21	37.37229401769951	40.0	35.0	40.0	33.0	40.0
22	37.39304527819441	40.0	35.0	40.0	33.0	40.0
23	37.43983994066957	39.0	35.0	40.0	33.0	40.0
24	37.42643414156285	39.0	35.0	40.0	33.0	40.0
25	37.431254738962714	39.0	35.0	40.0	34.0	40.0
26	37.283207606692386	39.0	35.0	40.0	33.0	40.0
27	37.19264236259084	39.0	35.0	40.0	33.0	40.0
28	37.116119391420334	39.0	35.0	40.0	33.0	40.0
29	37.03238046744851	39.0	35.0	40.0	33.0	40.0
30	36.90640071285068	39.0	35.0	40.0	32.0	40.0
31	36.81578123011019	39.0	35.0	40.0	32.0	40.0
32	36.72475772485513	38.0	35.0	40.0	32.0	40.0
33	36.666783261291876	38.0	35.0	40.0	32.0	40.0
34	36.22580873684851	38.0	35.0	40.0	30.0	40.0
35	36.121084994161045	38.0	35.0	40.0	30.0	40.0
36	36.35382963532817	38.0	35.0	40.0	31.0	40.0
37	36.43604877049861	38.0	35.0	40.0	31.0	40.0
38	36.36388038719747	38.0	35.0	40.0	31.0	40.0
39	36.26535977374739	38.0	35.0	40.0	31.0	40.0
40	36.152961817105094	38.0	35.0	40.0	31.0	40.0
41	36.02847417853368	38.0	35.0	40.0	31.0	40.0
42	35.919805405046404	38.0	35.0	40.0	31.0	40.0
43	35.84867751808416	38.0	35.0	40.0	31.0	40.0
44	35.78155775584864	37.0	34.0	40.0	30.0	40.0
45	35.58477775994421	37.0	34.0	40.0	30.0	40.0
46	35.321917391232155	37.0	34.0	40.0	29.0	40.0
47	35.19276301588971	36.0	34.0	40.0	29.0	40.0
48	35.08627264324812	36.0	34.0	39.0	29.0	40.0
49	35.432139160850774	36.0	34.0	40.0	30.0	40.0
50	35.53762445830543	36.0	34.0	40.0	30.0	40.0
51	35.55326732454077	36.0	34.0	40.0	31.0	40.0
52	35.12972222068485	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	8.0
16	32.0
17	39.0
18	124.0
19	196.0
20	348.0
21	570.0
22	976.0
23	1545.0
24	2299.0
25	3342.0
26	4629.0
27	6470.0
28	8623.0
29	11932.0
30	15783.0
31	21258.0
32	28689.0
33	43441.0
34	100333.0
35	68743.0
36	92655.0
37	156252.0
38	298749.0
39	36377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.2981077356475	0.48748360388083	0.13603936175511808	0.0783692987165367
8	98.82202531505455	0.8606233015834361	0.1781019797103214	0.13924940365169938
9	96.74944516086184	1.6612520270307667	0.784799898164188	0.8045029139432045
10	59.29489769375094	27.528765849581866	5.218863977241909	7.957472479425292
11	35.031740672891196	20.608690358251742	23.323721656160238	21.035847312696824
12	32.290586275410526	15.969404979992582	28.591621790649924	23.148386953946968
13	27.07526441336484	16.547544594676864	28.817763707709084	27.55942728424921
14	22.885052827327417	19.10771904385028	34.1578344393219	23.849393689500396
15	26.615010820055012	20.998433720936667	31.334215172429058	21.05234028657926
16	32.93890404742007	21.189486559333197	26.9350187898142	18.93659060343253
17	31.696175069043576	21.385188423924774	25.2894848989667	21.62915160806495
18	29.384170065805858	23.3301417399534	26.83561818212007	20.450070012120676
19	29.432652767554224	25.49692002014578	24.3030058168173	20.767421395482696
20	29.781993878782174	25.02349418594998	25.17491961058871	20.019592324679135
21	28.951589247466558	24.873507745609714	26.353890515433108	19.821012491490624
22	26.06033771854574	23.064593791336208	30.377844069447598	20.497224420670456
23	29.601346003774566	21.365264025945994	28.97018535224675	20.06320461803269
24	32.129087960682526	20.90943807663145	25.256831024501476	21.704642938184556
25	28.470415036279007	22.07988576678492	23.23904296475042	26.210656232185652
26	24.415135900997882	22.817752638599092	26.671131207695247	26.09598025270778
27	22.05298782951357	22.40232894074152	26.978409700967994	28.566273528776918
28	22.406867275836685	25.308191694846776	24.34185839287592	27.943082636440618
29	29.012137279102074	24.77410713791558	22.52586020820996	23.687895374772392
30	27.309708162915157	21.78777195419602	27.10371202603455	23.79880785685427
31	29.471616034712728	20.64034801281803	25.861204429857814	24.026831522611424
32	33.06564535678509	22.35118965259598	24.769015347320998	19.81414964329793
33	33.923944145271	20.540836714024007	25.590453999546163	19.944765141158825
34	26.938782287210195	23.65834085110387	30.069347974076145	19.33352888760979
35	23.376521310804005	29.305911458189204	29.083090274126512	18.234476956880282
36	25.444563129901542	32.596868548784336	23.37519301760542	18.583375303708706
37	28.220031768345667	29.24669171975227	23.160120210534473	19.37315630136759
38	27.437999147678532	29.0398100540726	21.97804995489338	21.54414084335549
39	27.60303957760276	27.21683833011407	22.627806711201387	22.552315381081783
40	27.001654831943238	24.680351776315426	22.91493942429559	25.40305396744575
41	22.112096876850618	27.071279533769086	22.33491806091331	28.481705528466982
42	22.017677368651174	26.87956254877327	23.370654682510253	27.73210540006531
43	21.2922079000238	23.250997603537687	23.786853218067	31.66994127837151
44	22.933646220175667	22.19500451066232	25.885556471831883	28.985792797330127
45	26.003442493206336	23.67737972028359	24.86609144191761	25.453086344592464
46	25.471239684973128	26.34735974054006	23.246016504042995	24.935384070443817
47	21.874221703203954	26.031558032576392	26.280502316211262	25.813717948008392
48	25.041647526330646	23.004820597399867	27.72557462517226	24.227957251097227
49	26.42229761516025	22.43420797750757	27.463790173951065	23.679704233381116
50	24.29946370162107	22.95179956055633	27.497772341614873	25.25096439620772
51	21.775817315408755	22.49508808244273	28.944394325974223	26.784700276174295
52	20.515045687751478	24.132984287398372	29.467299081817327	25.884670943032827
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	3.5
10	4.0
11	8.0
12	12.0
13	92.5
14	568.5
15	964.0
16	1275.5
17	1587.0
18	1312.5
19	1038.0
20	1451.0
21	1864.0
22	1827.5
23	1791.0
24	2165.5
25	2540.0
26	3077.0
27	3614.0
28	4524.5
29	5435.0
30	6927.0
31	8419.0
32	8403.5
33	8388.0
34	10579.0
35	12770.0
36	14315.0
37	15860.0
38	17182.0
39	21151.5
40	23799.0
41	25323.0
42	26847.0
43	26506.5
44	26166.0
45	29456.5
46	32747.0
47	34174.0
48	35601.0
49	40238.0
50	44875.0
51	47579.0
52	50283.0
53	50238.0
54	50193.0
55	51310.0
56	52427.0
57	55873.0
58	59319.0
59	66803.0
60	74287.0
61	78059.0
62	81831.0
63	79633.5
64	81369.5
65	85303.0
66	64431.0
67	43559.0
68	33788.5
69	24018.0
70	18650.5
71	13283.0
72	11011.0
73	8739.0
74	6647.5
75	4556.0
76	3801.5
77	3047.0
78	2220.0
79	1393.0
80	856.5
81	320.0
82	341.5
83	363.0
84	206.5
85	50.0
86	26.5
87	3.0
88	2.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	903415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.056446926384884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.22455254200065	4.6025359330983
2	9.8934885253102	1.3962575339129857
3	4.630660873111736	0.9802803805560013
4	2.392194387362939	0.6752157092808952
5	1.3443348130951074	0.4743113629948584
6	0.8235423300757658	0.34867696462865905
7	0.5019686583318954	0.24794806373593534
8	0.4000062746082292	0.22580984375951252
9	0.3105931073428603	0.19725153998992712
>10	6.429904782820123	13.203345085038437
>50	4.96635241337119	24.867862499515727
>100	2.930242043012439	38.352030904955086
>500	0.10353103578095342	4.9319526463474705
>1k	0.04392225760404085	5.2062451918553485
>5k	0.001568652057287173	0.6226374368368912
>10k+	0.003137304114574346	3.6676389034939647
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	18574	2.055976489210385	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14560	1.6116624142835794	No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5625	0.6226374368368912	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4623	0.5117249547550129	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3722	0.41199227376122827	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3204	0.35465428402229315	No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2977	0.3295274043490533	No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2131	0.2358827338487849	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTC	2124	0.23510789614961008	No Hit
TCGGGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	1817	0.20112572848580113	No Hit
TCGGGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	1756	0.19437357139299216	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCT	1523	0.1685825451204596	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC	1519	0.16813978072093114	No Hit
TCGGGTGGGGCTCCCACGGTGACTTGTTTACAGCTGGGTGTGACTCAGTAAA	1472	0.16293729902647178	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCGCT	1460	0.1616090058278864	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	1430	0.158288272831423	No Hit
TCGGGTGGGGCCCGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGTAT	1400	0.15496753983495956	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGGTAAGAGCACTGACTGCTCT	1294	0.1432342832474555	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	1272	0.140799079050049	No Hit
TCGGGTGGGAGCCGGGCGTGGTGGCCCACACCTTTAATCTCATCATCTCATC	1252	0.13858525705240668	No Hit
TCGGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	1251	0.13847456595252458	No Hit
TCGGGTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1247	0.13803180155299613	No Hit
TCGGGTGGGCGAGCAAGATGTTGGACACGTGTACAATCCTGTATCATTTATG	1221	0.13515383295606118	No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1117	0.12364195856832132	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCTGTTAAGAGCACTGACTGCTC	1069	0.11832878577397983	No Hit
TCGGGTGGGCCAGAGTGTGGGACTCACTCTGAGTCAAATAAAACTGCTACTG	1052	0.1164470370759839	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC	1030	0.11401183287857741	No Hit
TCGGGTGGGGGAGAGATGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTC	1025	0.11345837737916684	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	1024	0.11334768627928471	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1016	0.1124621574802278	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC	1006	0.11135524648140667	No Hit
TCGGGTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	999	0.11058040878223185	No Hit
TCGGGTGGGGGGCCAGGCAGTGGTGGTGCACGCCATTAATCCCAGCACTTGG	990	0.10958418888329284	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	909	0.1006182097928416	No Hit
TCGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	906	0.10028613649319526	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0033207329964634194	0.0	0.0	0.0	0.0
8	0.004981099494695129	0.0	0.0	0.0	0.0
9	0.006088010493516269	0.0	0.0	0.0	0.0
10	0.011511874387739854	0.0	0.0	0.0	0.0
11	0.02590171737241467	0.0	0.0	0.0	0.0
12	0.03420354986357322	0.0	0.0	0.0	0.0
13	0.04350160225367079	0.0	0.0	0.0	0.0
14	0.05080721484589032	0.0	0.0	0.0	0.0
15	0.059109047337048866	0.0	0.0	0.0	0.0
16	0.06862848192691066	0.0	0.0	0.0	0.0
17	0.07715169661783344	0.0	0.0	0.0	0.0
18	0.08700320450734159	0.0	0.0	0.0	0.0
19	0.10039682759307737	0.0	0.0	0.0	0.0
20	0.10947349778341073	0.0	0.0	0.0	0.0
21	0.11843947687386196	0.0	0.0	0.0	0.0
22	0.12718407376454896	0.0	0.0	0.0	0.0
23	0.1362607439548823	0.0	0.0	0.0	0.0
24	0.14611225184439044	0.0	0.0	0.0	0.0
25	0.15474615763519534	0.0	0.0	0.0	0.0
26	0.16360144562576445	0.0	0.0	0.0	0.0
27	0.17245673361633357	0.0	0.0	0.0	0.0
28	0.18264031480548806	0.0	0.0	0.0	0.0
29	0.19182767609570353	0.0	0.0	0.0	0.0
30	0.2011257284858011	0.0	0.0	0.0	0.0
31	0.2092061787771954	0.0	0.0	0.0	0.0
32	0.2193897599663499	0.0	0.0	0.0	0.0
33	0.22724882805798	0.0	0.0	0.0	0.0
34	0.2374324092471345	0.0	0.0	0.0	0.0
35	0.2502725768334597	0.0	0.0	0.0	0.0
36	0.2642196554186061	0.0	0.0	0.0	0.0
37	0.2772812052046955	0.0	0.0	0.0	0.0
38	0.2873540952939679	0.0	0.0	0.0	0.0
39	0.29742698538324025	0.0	0.0	0.0	0.0
40	0.3099350796699191	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	40	5.6097633E-9	46.000004	9
CCGGGTC	20	6.3107326E-4	46.000004	34
CGAACAA	20	6.3107326E-4	46.000004	27
CGAAAGG	40	5.6097633E-9	46.000004	36
CGCGGAC	35	1.0192525E-7	46.000004	38
CGACGAA	20	6.3107326E-4	46.000004	24
ACGTTAA	40	5.6097633E-9	46.000004	12
GGCACGA	20	6.3107326E-4	46.000004	9
CGGTTAG	20	6.3107326E-4	46.000004	29
CGTGAAA	20	6.3107326E-4	46.000004	34
ACCGACC	20	6.3107326E-4	46.000004	46
CGACCGA	40	5.6097633E-9	46.000004	13
CGCGCAC	35	1.0192525E-7	46.000004	22
CCTACCG	20	6.3107326E-4	46.000004	27
ATTCGGT	80	0.0	46.000004	18
TTCGTCG	20	6.3107326E-4	46.000004	17
CGTCTAC	20	6.3107326E-4	46.000004	27
CGATTGT	20	6.3107326E-4	46.000004	14
CGATTAC	20	6.3107326E-4	46.000004	25
TCGATTG	20	6.3107326E-4	46.000004	13
>>END_MODULE