FastQCFastQC Report
Fri 17 Jun 2016
SRR1527814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910047
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT178731.963964498536889No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC137581.5117900504039903No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC54580.5997492437203792No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC43010.47261295295737477No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT36750.40382529693521324No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC29050.3192142823392638No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG27890.30646768793260126No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTC20690.22735089506366155No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA19690.21636245160964215No Hit
TCGGGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA16490.18119943255678003No Hit
TCGGGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT16240.17845232169327518No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCT14700.1615301187740853No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC14350.1576841635651785No Hit
TCGGGTGGGGCTCCCACGGTGACTTGTTTACAGCTGGGTGTGACTCAGTAAA14300.15713474139247754No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCGCT14280.15691497252339714No Hit
TCGGGTGGGGCCCGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGTAT14010.1539480927908119No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT13170.1447178002894356No Hit
TCGGGTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT12490.1372456587407024No Hit
TCGGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA12100.13296016579363484No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGGTAAGAGCACTGACTGCTCT12010.13197120588277308No Hit
TCGGGTGGGAGCCGGGCGTGGTGGCCCACACCTTTAATCTCATCATCTCATC12010.13197120588277308No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT11650.12801536623932608No Hit
TCGGGTGGGCGAGCAAGATGTTGGACACGTGTACAATCCTGTATCATTTATG11190.12296068225047718No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10560.11603796287444496No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCTGTTAAGAGCACTGACTGCTC10530.11570830957082436No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC10080.11076351001651563No Hit
TCGGGTGGGGGAGAGATGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTC10020.11010420340927447No Hit
TCGGGTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA9850.10823616802209117No Hit
TCGGGTGGGCCAGAGTGTGGGACTCACTCTGAGTCAAATAAAACTGCTACTG9740.10702743924214903No Hit
TCGGGTGGGGGGCCAGGCAGTGGTGGTGCACGCCATTAATCCCAGCACTTGG9700.10658790150398825No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC9510.10450009724772458No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT9420.10351113733686282No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC9120.10021460430065701No Hit
TCGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT9110.1001047198661168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG351.0192525E-746.00000423
ACGGATA351.0192525E-746.00000426
TCGACAC351.0192525E-746.00000418
TGTCGGC351.0192525E-746.00000411
CGCCACT351.0192525E-746.00000426
AACCGGA253.4165903E-546.033
ACTATCG206.3107576E-446.014
AACCGCG405.6097633E-946.017
GGTACGT206.3107576E-446.09
ACACGTA253.4165903E-546.035
TCGCACT206.3107576E-446.019
CGTATAT206.3107576E-446.035
CGAGTGT206.3107576E-446.038
CAACGAC650.046.024
CGCGGAC206.3107576E-446.038
TCCGACG301.8609735E-646.034
GCGCGAC206.3107576E-446.09
CGGTTGT206.3107576E-446.036
ACCGATT551.8189894E-1246.010
ATAGCGG206.3107576E-446.020
Produced by FastQC (version 0.11.5)