##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527814_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	910047
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51268890507853	31.0	31.0	33.0	30.0	33.0
2	32.04907438846565	31.0	31.0	34.0	31.0	34.0
3	32.69580691986238	34.0	31.0	34.0	31.0	34.0
4	36.38133854625091	37.0	37.0	37.0	35.0	37.0
5	36.3541564336787	37.0	37.0	37.0	35.0	37.0
6	31.484655188138635	33.0	32.0	35.0	28.0	35.0
7	34.68575029641326	35.0	35.0	35.0	32.0	35.0
8	36.04224726854767	37.0	35.0	37.0	35.0	37.0
9	38.60504896999825	39.0	39.0	39.0	37.0	39.0
10	37.95427049372175	39.0	38.0	39.0	35.0	39.0
11	37.6192328528087	39.0	37.0	39.0	35.0	39.0
12	36.922490816408384	39.0	35.0	39.0	34.0	39.0
13	36.7060184803642	39.0	35.0	39.0	33.0	39.0
14	37.509782461784944	40.0	36.0	40.0	33.0	40.0
15	37.57916129606493	40.0	36.0	40.0	33.0	40.0
16	37.64019770407462	40.0	36.0	40.0	33.0	40.0
17	37.46052896169099	40.0	36.0	40.0	33.0	40.0
18	37.41427970203737	40.0	36.0	40.0	33.0	40.0
19	37.42742407809707	40.0	36.0	40.0	33.0	40.0
20	37.39868490308742	40.0	35.0	40.0	33.0	40.0
21	37.4289690532467	40.0	35.0	40.0	33.0	40.0
22	37.34631837696295	40.0	35.0	40.0	33.0	40.0
23	37.41641365775614	39.0	35.0	40.0	33.0	40.0
24	37.444175960142715	39.0	35.0	40.0	34.0	40.0
25	37.41956953871613	39.0	35.0	40.0	34.0	40.0
26	37.22905190611034	39.0	35.0	40.0	33.0	40.0
27	37.13212724177982	39.0	35.0	40.0	33.0	40.0
28	37.07796630283931	39.0	35.0	40.0	33.0	40.0
29	36.99657819870842	39.0	35.0	40.0	33.0	40.0
30	36.859683071313896	39.0	35.0	40.0	32.0	40.0
31	36.79689400657329	39.0	35.0	40.0	32.0	40.0
32	36.70617781279429	38.0	35.0	40.0	32.0	40.0
33	36.46325409566759	38.0	35.0	40.0	31.0	40.0
34	36.14485076045523	38.0	35.0	40.0	30.0	40.0
35	36.11736097146631	38.0	35.0	40.0	30.0	40.0
36	36.201537942545826	38.0	35.0	40.0	31.0	40.0
37	36.35691782951869	38.0	35.0	40.0	31.0	40.0
38	36.01819576351551	38.0	35.0	40.0	30.0	40.0
39	36.08354293789222	38.0	35.0	40.0	31.0	40.0
40	36.04618113130421	38.0	35.0	40.0	31.0	40.0
41	35.74258472364614	38.0	34.0	40.0	30.0	40.0
42	35.7760390397419	38.0	34.0	40.0	30.0	40.0
43	35.805902332516894	37.0	34.0	40.0	30.0	40.0
44	35.73427526270621	37.0	34.0	40.0	30.0	40.0
45	35.53311092723782	37.0	34.0	40.0	30.0	40.0
46	35.09539177646869	37.0	34.0	40.0	29.0	40.0
47	34.88231596829614	36.0	34.0	40.0	28.0	40.0
48	34.947867527721094	36.0	34.0	39.0	28.0	40.0
49	34.871216541563236	36.0	34.0	39.0	28.0	40.0
50	34.7742479234589	36.0	34.0	39.0	28.0	40.0
51	35.15017026593132	36.0	34.0	39.0	30.0	40.0
52	34.93988222586306	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	14.0
17	58.0
18	99.0
19	224.0
20	407.0
21	623.0
22	1008.0
23	1622.0
24	2419.0
25	3454.0
26	4991.0
27	7085.0
28	9309.0
29	12399.0
30	16396.0
31	22012.0
32	30836.0
33	47562.0
34	100240.0
35	71190.0
36	99009.0
37	165774.0
38	283410.0
39	29896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.29113551278121	0.4834915119768539	0.1463660668075385	0.07900690843439954
8	98.80808353854252	0.8766580187616684	0.18328723681304374	0.13197120588277308
9	96.67456735751011	1.707054690581915	0.8100680514303108	0.8083099004776677
10	59.276389021665906	27.5055024630596	5.277969159834602	7.940139355439883
11	35.04071767721887	20.509050631450904	23.390330389529332	21.059901301800895
12	32.27723403296753	15.94851694472923	28.595116515960168	23.179132506343077
13	27.21672616908797	16.52189392415996	28.854883319213183	27.406496587538886
14	23.031008288582896	19.06385054837827	34.12922629270796	23.775914870330872
15	26.67763313323378	21.05506638668113	31.188499055543282	21.07880142454181
16	32.829403316532	21.238573392363254	26.924873110949214	19.00715018015553
17	31.673419065169163	21.337689152318507	25.322758055353184	21.66613372715915
18	29.45902794031517	23.199021588994853	26.78510011021409	20.556850360475888
19	29.411667749028346	25.463190362695553	24.36874139467522	20.75640049360088
20	29.83142628897189	24.966622603008414	25.114966589637678	20.086984518382017
21	28.924330281842586	24.80564190640703	26.478742306716025	19.791285505034356
22	26.160187330984	23.0438647674241	30.310412539132592	20.485535362459302
23	29.661325184303667	21.364720723215395	28.848510022009854	20.125444070471087
24	32.067134994126675	20.952873862558747	25.186721125392424	21.79327001792215
25	28.431498592929817	22.071057868439762	23.31418047639298	26.183263062237444
26	24.491811961360238	22.7721205608062	26.79773682018621	25.938330657647352
27	22.067102028796313	22.391370995124426	26.987397354202585	28.554129621876672
28	22.434225924595104	25.242102880400687	24.300942698563922	28.02272849644029
29	29.00520522566417	24.733997255086827	22.55608776250018	23.704709756748827
30	27.304963370023742	21.839751133732655	27.015857422748496	23.839428073495107
31	29.455291869540805	20.675855203082918	25.727352543330177	24.1415003840461
32	33.07169849469313	22.316319926333474	24.78201675298089	19.829964825992505
33	33.701995611215686	20.587288348843522	25.7058151941603	20.004900845780494
34	26.919488773656745	23.720313346453533	29.95570558443685	19.404492295452872
35	23.365166854019627	29.355956340716467	28.997952852984515	18.280923952279387
36	25.60087555917442	32.45557647022626	23.364287778543304	18.579260192056015
37	28.266232403381363	29.186404658220948	23.18429707476647	19.363065863631217
38	27.440340993377266	28.89466148451673	21.935240707348083	21.72975681475792
39	27.59055301539371	27.16057522303793	22.713002735023576	22.535869026544784
40	27.036515696442052	24.5830160420286	22.90870691293966	25.471761348589688
41	22.327528138656575	26.97695833292127	22.344779994879385	28.350733533542773
42	22.037762884774086	26.887732172074628	23.41659276938444	27.657912173766853
43	21.376917895449356	23.25242542418139	23.74031231353985	31.630344366829405
44	22.990131278933944	22.21006167813311	25.787129675720045	29.012677367212902
45	25.905255442850756	23.641965744626376	24.892780262997405	25.559998549525464
46	25.64395025751417	26.14820992761912	23.329014875055904	24.8788249398108
47	22.09325452421688	25.997118830126354	26.250622220610587	25.659004425046177
48	25.055409226116893	22.95397929997022	27.769334990390604	24.22127648352228
49	26.260951357457362	22.426534014177292	27.47759181668639	23.834922811678958
50	24.269625634719965	22.875082275970364	27.428803127750545	25.42648896155913
51	21.748766821933373	22.52202358779272	28.99696389307365	26.732245697200252
52	20.532675784877043	24.105568173951454	29.539683115267675	25.82207292590383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.5
8	7.0
9	4.0
10	1.0
11	7.5
12	14.0
13	93.5
14	536.0
15	899.0
16	1196.0
17	1493.0
18	1249.5
19	1006.0
20	1386.0
21	1766.0
22	1783.0
23	1800.0
24	2177.0
25	2554.0
26	3088.0
27	3622.0
28	4486.5
29	5351.0
30	6894.5
31	8438.0
32	8418.0
33	8398.0
34	10597.5
35	12797.0
36	14336.0
37	15875.0
38	17161.0
39	21141.5
40	23836.0
41	25341.5
42	26847.0
43	26770.0
44	26693.0
45	29848.0
46	33003.0
47	34632.5
48	36262.0
49	40821.0
50	45380.0
51	47926.5
52	50473.0
53	50810.0
54	51147.0
55	52188.5
56	53230.0
57	56460.5
58	59691.0
59	67201.0
60	74711.0
61	78456.0
62	82201.0
63	79857.0
64	81452.5
65	85392.0
66	65071.0
67	44750.0
68	34242.5
69	23735.0
70	18747.5
71	13760.0
72	11278.5
73	8797.0
74	6763.5
75	4730.0
76	3905.0
77	3080.0
78	2247.5
79	1415.0
80	875.0
81	335.0
82	356.5
83	378.0
84	209.0
85	40.0
86	21.5
87	3.0
88	2.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	910047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.84218727164641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49490721503207	7.860033602660082
2	10.245365819051576	2.221643497533644
3	3.708358248284669	1.2062014379477104
4	1.766512278425848	0.7661142776142331
5	0.9182215285449331	0.49777648846707917
6	0.587823936596094	0.3823978321998754
7	0.4114767556172658	0.3122915629632316
8	0.2706017087433743	0.23471315217785454
9	0.21790025235889693	0.21262638083527555
>10	4.390436712645309	13.77555225169689
>50	3.169181809889631	24.314788137315986
>100	1.7279996756833453	34.93863503753103
>500	0.06384984138888607	4.726569067311908
>1k	0.02432374910052803	4.475153481083944
>5k	0.0010134895458553347	0.5997492437203792
>10k+	0.0020269790917106694	3.475754548940879
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	17873	1.963964498536889	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	13758	1.5117900504039903	No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5458	0.5997492437203792	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4301	0.47261295295737477	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3675	0.40382529693521324	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2905	0.3192142823392638	No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2789	0.30646768793260126	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTC	2069	0.22735089506366155	No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1969	0.21636245160964215	No Hit
TCGGGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	1649	0.18119943255678003	No Hit
TCGGGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	1624	0.17845232169327518	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCT	1470	0.1615301187740853	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC	1435	0.1576841635651785	No Hit
TCGGGTGGGGCTCCCACGGTGACTTGTTTACAGCTGGGTGTGACTCAGTAAA	1430	0.15713474139247754	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCGCT	1428	0.15691497252339714	No Hit
TCGGGTGGGGCCCGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGTAT	1401	0.1539480927908119	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	1317	0.1447178002894356	No Hit
TCGGGTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1249	0.1372456587407024	No Hit
TCGGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	1210	0.13296016579363484	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGGTAAGAGCACTGACTGCTCT	1201	0.13197120588277308	No Hit
TCGGGTGGGAGCCGGGCGTGGTGGCCCACACCTTTAATCTCATCATCTCATC	1201	0.13197120588277308	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	1165	0.12801536623932608	No Hit
TCGGGTGGGCGAGCAAGATGTTGGACACGTGTACAATCCTGTATCATTTATG	1119	0.12296068225047718	No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1056	0.11603796287444496	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCTGTTAAGAGCACTGACTGCTC	1053	0.11570830957082436	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1008	0.11076351001651563	No Hit
TCGGGTGGGGGAGAGATGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTC	1002	0.11010420340927447	No Hit
TCGGGTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	985	0.10823616802209117	No Hit
TCGGGTGGGCCAGAGTGTGGGACTCACTCTGAGTCAAATAAAACTGCTACTG	974	0.10702743924214903	No Hit
TCGGGTGGGGGGCCAGGCAGTGGTGGTGCACGCCATTAATCCCAGCACTTGG	970	0.10658790150398825	No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC	951	0.10450009724772458	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	942	0.10351113733686282	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	912	0.10021460430065701	No Hit
TCGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	911	0.1001047198661168	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0029668797325852403	0.0	0.0	0.0	0.0
8	0.004505261816147957	0.0	0.0	0.0	0.0
9	0.005604106161549898	0.0	0.0	0.0	0.0
10	0.01263670997212232	0.0	0.0	0.0	0.0
11	0.028130415242289684	0.0	0.0	0.0	0.0
12	0.035932210094643466	0.0	0.0	0.0	0.0
13	0.04285492947067569	0.0	0.0	0.0	0.0
14	0.0501073021503285	0.0	0.0	0.0	0.0
15	0.058678288044463636	0.0	0.0	0.0	0.0
16	0.06812834941492033	0.0	0.0	0.0	0.0
17	0.07779817965445741	0.0	0.0	0.0	0.0
18	0.08746800989399449	0.0	0.0	0.0	0.0
19	0.09966518212795603	0.0	0.0	0.0	0.0
20	0.1095547812365735	0.0	0.0	0.0	0.0
21	0.11647750061260573	0.0	0.0	0.0	0.0
22	0.1259275619830624	0.0	0.0	0.0	0.0
23	0.1355973922225995	0.0	0.0	0.0	0.0
24	0.14581664463483754	0.0	0.0	0.0	0.0
25	0.15449751496351288	0.0	0.0	0.0	0.0
26	0.1650464206793715	0.0	0.0	0.0	0.0
27	0.17504590422252916	0.0	0.0	0.0	0.0
28	0.185155272200227	0.0	0.0	0.0	0.0
29	0.1950448713088445	0.0	0.0	0.0	0.0
30	0.2041652793756806	0.0	0.0	0.0	0.0
31	0.21405487848429805	0.0	0.0	0.0	0.0
32	0.22218632664027244	0.0	0.0	0.0	0.0
33	0.23064742809986738	0.0	0.0	0.0	0.0
34	0.24185564042296717	0.0	0.0	0.0	0.0
35	0.2555911947404914	0.0	0.0	0.0	0.0
36	0.26943663349255587	0.0	0.0	0.0	0.0
37	0.28031519251203507	0.0	0.0	0.0	0.0
38	0.2897652538824918	0.0	0.0	0.0	0.0
39	0.29943508412202885	0.0	0.0	0.0	0.0
40	0.30877526105794534	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTCG	35	1.0192525E-7	46.000004	23
ACGGATA	35	1.0192525E-7	46.000004	26
TCGACAC	35	1.0192525E-7	46.000004	18
TGTCGGC	35	1.0192525E-7	46.000004	11
CGCCACT	35	1.0192525E-7	46.000004	26
AACCGGA	25	3.4165903E-5	46.0	33
ACTATCG	20	6.3107576E-4	46.0	14
AACCGCG	40	5.6097633E-9	46.0	17
GGTACGT	20	6.3107576E-4	46.0	9
ACACGTA	25	3.4165903E-5	46.0	35
TCGCACT	20	6.3107576E-4	46.0	19
CGTATAT	20	6.3107576E-4	46.0	35
CGAGTGT	20	6.3107576E-4	46.0	38
CAACGAC	65	0.0	46.0	24
CGCGGAC	20	6.3107576E-4	46.0	38
TCCGACG	30	1.8609735E-6	46.0	34
GCGCGAC	20	6.3107576E-4	46.0	9
CGGTTGT	20	6.3107576E-4	46.0	36
ACCGATT	55	1.8189894E-12	46.0	10
ATAGCGG	20	6.3107576E-4	46.0	20
>>END_MODULE