FastQCFastQC Report
Fri 17 Jun 2016
SRR1527810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742155
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC349212.004471473548565No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG180181.0342363337360911No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT91860.527277997652333No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA72790.41781586598207393No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC65790.377635744236305No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC64750.3716661261483622No Hit
AAAGTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC60200.34554904701361244No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGC42990.2467633476929435No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT41590.2387273233437897No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG28950.16617350350571564No Hit
AAAGTTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT25170.14447623776300042No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG22260.12777278715154508No Hit
AAAGTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG22090.12679698419486213No Hit
AAAGTTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC21950.12599338175994673No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC20730.11899056054139844No Hit
AAAGTTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC20270.1163501525409622No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT19850.11393934523621606No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA19790.1135949441926809No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC19420.11147113775754741No Hit
AAAGTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA18590.10670692332197768No Hit
AAAGTTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA17500.10045030436442223No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGCTGGTGAGATGGCTCAG17490.1003929041904997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG302.5284717E-644.021
TCGCAAT302.5284717E-644.021
ACACGCT254.4437897E-544.024
CGTATCT254.4437897E-544.021
AATCACG254.4437897E-544.031
CGACGTT207.857602E-444.015
CGGTTAT207.857602E-444.016
CGTCTAT207.857602E-444.011
CGATTTA302.5284717E-644.038
ATCGAGG254.4437897E-544.026
CGATTAG700.043.99999632
GGCGATA603.6379788E-1240.33333231
TAATCGG603.6379788E-1240.33333240
TTGGGAT185300.040.2245035
TACCACG557.8216544E-1140.020
CACGTTA557.8216544E-1140.028
TTGGGAC102300.039.9784935
AAAGTTG1913300.039.839861
AAGTTGG1898850.039.8360062
AGTTGGG1845100.039.8148653
Produced by FastQC (version 0.11.5)