##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1742155
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.498549784605846	31.0	31.0	33.0	3.0	34.0
2	30.297313384859557	34.0	31.0	34.0	16.0	34.0
3	32.339439372501296	34.0	31.0	34.0	28.0	34.0
4	36.1695360056941	37.0	35.0	37.0	35.0	37.0
5	34.97173271035011	35.0	35.0	37.0	33.0	37.0
6	35.62441114596577	35.0	35.0	37.0	35.0	37.0
7	36.18069230349768	37.0	35.0	37.0	35.0	37.0
8	36.653359201678384	37.0	37.0	37.0	35.0	37.0
9	38.751540477167644	39.0	39.0	39.0	39.0	39.0
10	38.34075268848065	39.0	39.0	39.0	37.0	39.0
11	38.10900235627714	39.0	38.0	39.0	37.0	39.0
12	37.637618351983605	39.0	37.0	39.0	35.0	39.0
13	37.51343479770744	39.0	37.0	39.0	35.0	39.0
14	38.25838630891052	40.0	38.0	40.0	35.0	40.0
15	38.3088393397832	40.0	38.0	40.0	35.0	40.0
16	38.31223054205854	40.0	38.0	40.0	35.0	40.0
17	38.1727056432981	40.0	38.0	40.0	34.0	40.0
18	38.11137413146362	40.0	38.0	40.0	34.0	40.0
19	38.1276843908837	40.0	38.0	40.0	34.0	40.0
20	38.075071965468055	40.0	38.0	40.0	34.0	40.0
21	38.0778105277659	40.0	38.0	40.0	34.0	40.0
22	38.163579589646154	40.0	38.0	40.0	34.0	40.0
23	38.14535733043271	40.0	38.0	40.0	34.0	40.0
24	38.074708622367126	40.0	37.0	40.0	35.0	40.0
25	38.031559763626085	40.0	37.0	40.0	35.0	40.0
26	37.884763984834876	40.0	37.0	40.0	34.0	40.0
27	37.78867035367117	40.0	37.0	40.0	34.0	40.0
28	37.65639280087019	40.0	36.0	40.0	34.0	40.0
29	37.66399200989579	40.0	36.0	40.0	34.0	40.0
30	37.816553636157515	40.0	37.0	40.0	34.0	40.0
31	37.85724921146511	40.0	37.0	40.0	34.0	40.0
32	37.857794513117376	40.0	37.0	40.0	34.0	40.0
33	37.74125781001116	40.0	36.0	40.0	34.0	40.0
34	37.316335802497484	40.0	36.0	40.0	33.0	40.0
35	37.40295438695179	40.0	35.0	40.0	33.0	40.0
36	37.427734042034146	40.0	35.0	40.0	33.0	40.0
37	37.36746845142941	40.0	35.0	40.0	33.0	40.0
38	37.29303879390755	40.0	35.0	40.0	33.0	40.0
39	37.187021820676115	40.0	35.0	40.0	33.0	40.0
40	37.05717459123901	40.0	35.0	40.0	33.0	40.0
41	36.91399559740666	40.0	35.0	40.0	33.0	40.0
42	36.8376562360984	40.0	35.0	40.0	33.0	40.0
43	36.778034101443325	40.0	35.0	40.0	33.0	40.0
44	36.426652048755706	39.0	35.0	40.0	32.0	40.0
45	36.41461465828242	39.0	35.0	40.0	32.0	40.0
46	36.23751445766881	39.0	35.0	40.0	31.0	40.0
47	36.188870106276426	39.0	35.0	40.0	31.0	40.0
48	36.12581486721905	39.0	35.0	40.0	31.0	40.0
49	36.075486968725514	39.0	35.0	40.0	31.0	40.0
50	35.5909887466959	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	2.0
13	4.0
14	5.0
15	13.0
16	25.0
17	88.0
18	149.0
19	298.0
20	519.0
21	895.0
22	1455.0
23	2346.0
24	3661.0
25	5122.0
26	7368.0
27	9844.0
28	13033.0
29	16983.0
30	22058.0
31	28634.0
32	37333.0
33	60742.0
34	118608.0
35	129748.0
36	138523.0
37	314250.0
38	552685.0
39	277762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.4697371933037	0.30760753205082214	0.19602159394542965	0.02663368070005252
8	99.1109861062879	0.7443654554273299	0.07094661496824335	0.07370182331652465
9	96.99877450628675	1.5885498133059344	0.6591261971523774	0.7535494832549343
10	53.14722283608519	31.044137863737724	7.33166681495045	8.47697248522663
11	34.445499969864905	23.407790925606506	25.73226836877316	16.414440735755427
12	34.081008865456866	18.517353507581127	24.461945119693713	22.939692507268298
13	24.99634073891244	18.645815096819742	28.822349331718478	27.535494832549347
14	24.718409096779563	20.41000944232861	31.540477167645818	23.331104293246007
15	28.056631011591964	21.239441955509125	28.61783251203251	22.086094520866396
16	32.074069184429625	22.56636177607618	25.530908558652932	19.828660480841258
17	30.07378792357741	22.489847344237454	25.270713570262117	22.16565116192302
18	29.78942746196521	23.457097675005954	25.124859728324978	21.628615134703857
19	28.309191776851083	26.031380675083444	24.324529103323183	21.334898444742286
20	29.29463796275303	25.296715849049022	23.598876104594595	21.809770083603354
21	26.787283565469206	27.115210759088598	26.2017443912855	19.89576128415669
22	26.413608433233556	24.58093568023511	27.195169201362678	21.810286685168656
23	30.825615401614666	23.84110483854766	25.432409860201876	19.900869899635794
24	31.937743771363625	23.142429921562663	23.19850989148497	21.72131641558874
25	28.574495380721004	22.453914835361953	22.91845444291696	26.053135341000083
26	25.461741349076288	22.6431058086106	27.113029552479546	24.782123289833567
27	24.48209258074052	22.154687728703816	25.0540853138785	28.309134376677157
28	26.487999058637147	27.346763060692076	21.52345801607779	24.641779864592987
29	30.50170622016985	24.040226041884907	22.957601361532127	22.50046637641312
30	30.18921967333561	21.66632704897096	26.543218025950623	21.60123525174281
31	32.068788368428756	22.847450427774795	22.800152684462635	22.283608519333814
32	33.93676222838955	22.467576076755513	22.639948799044863	20.955712895810073
33	33.34387583194377	22.12902985096045	23.70110581435062	20.82598850274516
34	27.883856488085158	23.828936001676084	27.959452517141127	20.32775499309763
35	25.361692845929323	28.730796054312048	26.289853658256586	19.617657441502047
36	26.272461405558055	30.507848038779557	22.53817829068022	20.681512264982164
37	29.510060815484273	28.87911810372785	21.870843868656923	19.739977212130952
38	28.592920836550135	29.09844416828583	21.305337355172185	21.00329763999185
39	29.503344995135333	25.548702612568917	21.283295688385937	23.664656703909813
40	26.95942668706286	24.761401827047536	22.31121800299055	25.967953482899052
41	23.838636631068994	27.798215428592748	22.723810453145674	25.639337487192588
42	22.509937405110335	25.44934291150902	23.107301015122076	28.933418668258565
43	23.330358090985015	23.795299499757483	24.27786276192417	28.596479647333332
44	25.437231474811366	23.00128289388717	23.258894874451467	28.302590756849995
45	27.921453602004416	24.131205317552112	22.194638249753897	25.75270283068958
46	24.802787352445677	27.493994506803354	22.806638904115882	24.896579236635084
47	23.607543530856898	25.273813179653935	25.02664803074353	26.09199525874563
48	25.438666479159433	24.095904210589758	25.59806676214229	24.86736254810852
49	27.630492120391125	23.80488532880255	23.876807746727472	24.687814804078855
50	23.519836065103277	24.675818167729048	24.705092256429538	27.099253510738137
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	42.0
2	84.0
3	63.0
4	42.0
5	70.0
6	98.0
7	978.0
8	1858.0
9	2827.0
10	3796.0
11	4332.0
12	4868.0
13	4586.5
14	4305.0
15	4386.0
16	4467.0
17	4739.0
18	5011.0
19	5665.5
20	6320.0
21	7834.5
22	9349.0
23	10768.0
24	12187.0
25	13260.5
26	14334.0
27	16763.0
28	19192.0
29	23031.5
30	26871.0
31	27742.0
32	28613.0
33	33023.5
34	37434.0
35	42077.5
36	46721.0
37	51392.5
38	56064.0
39	58849.0
40	61634.0
41	63911.5
42	66189.0
43	76313.5
44	86438.0
45	94843.0
46	103248.0
47	102875.5
48	102503.0
49	106530.5
50	110558.0
51	112200.0
52	113842.0
53	123696.0
54	133550.0
55	137115.0
56	140680.0
57	145964.0
58	151248.0
59	155853.0
60	160458.0
61	122826.5
62	85195.0
63	66745.5
64	48296.0
65	41546.5
66	34797.0
67	29417.0
68	24037.0
69	20050.5
70	16064.0
71	14245.0
72	12426.0
73	8923.0
74	5420.0
75	3986.0
76	2552.0
77	1750.5
78	949.0
79	597.5
80	246.0
81	209.5
82	173.0
83	100.5
84	28.0
85	17.0
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1742155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.767205208606915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.74400682409055	5.939256094800691
2	9.664443135098715	1.6946031238464558
3	4.0881123522283636	1.0752395972348034
4	2.0467376323204585	0.7177667532292683
5	1.1365977997808052	0.49823930751647183
6	0.7592249172291821	0.3993768389301504
7	0.5360294702132361	0.32896362542542024
8	0.43468556861558105	0.30487820650182246
9	0.34619784801731535	0.2731668818711321
>10	6.807695945106579	17.153688774037818
>50	4.164879559088002	25.473158781868836
>100	2.1774142294031846	33.03132031967528
>500	0.057984400966659654	3.3730053944744585
>1k	0.03132490626934487	4.567361917004695
>5k	0.003332436837164348	2.076626049618498
>10k+	0.0013329747348657392	3.0933483339641983
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	34921	2.004471473548565	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	18018	1.0342363337360911	No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	9186	0.527277997652333	No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	7279	0.41781586598207393	No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	6579	0.377635744236305	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	6475	0.3716661261483622	No Hit
AAAGTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	6020	0.34554904701361244	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGC	4299	0.2467633476929435	No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	4159	0.2387273233437897	No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	2895	0.16617350350571564	No Hit
AAAGTTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	2517	0.14447623776300042	No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2226	0.12777278715154508	No Hit
AAAGTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG	2209	0.12679698419486213	No Hit
AAAGTTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC	2195	0.12599338175994673	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	2073	0.11899056054139844	No Hit
AAAGTTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	2027	0.1163501525409622	No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT	1985	0.11393934523621606	No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	1979	0.1135949441926809	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC	1942	0.11147113775754741	No Hit
AAAGTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA	1859	0.10670692332197768	No Hit
AAAGTTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	1750	0.10045030436442223	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGCTGGTGAGATGGCTCAG	1749	0.1003929041904997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.011939236175885612	0.0	0.0	0.0	0.0
8	0.028470486265573386	0.0	0.0	0.0	0.0
9	0.04574793861625401	0.0	0.0	0.0	0.0
10	0.12530457967287642	0.0	0.0	0.0	0.0
11	0.18844477098765608	0.0	0.0	0.0	0.0
12	0.21605425464439157	0.0	0.0	0.0	0.0
13	0.23344650734291725	0.0	0.0	0.0	0.0
14	0.2491167548237671	0.0	0.0	0.0	0.0
15	0.27110102143609494	0.0	0.0	0.0	0.0
16	0.299858508571281	0.0	0.0	0.0	0.0
17	0.32304817883598186	0.0	0.0	0.0	0.0
18	0.34359744110024654	0.0	0.0	0.0	0.0
19	0.3718957268440523	0.0	0.0	0.0	0.0
20	0.3943965950216829	0.0	0.0	0.0	0.0
21	0.4177010656342289	0.0	0.0	0.0	0.0
22	0.44169433833384514	0.0	0.0	0.0	0.0
23	0.47211643051278446	0.0	0.0	0.0	0.0
24	0.49932411295206225	0.0	0.0	0.0	0.0
25	0.5250393908693544	0.0	0.0	0.0	0.0
26	0.5529358753957024	0.0	0.0	0.0	0.0
27	0.5767569475735511	0.0	0.0	0.0	0.0
28	0.6057440354044272	0.0	0.0	0.0	0.0
29	0.6291633063648183	0.0	0.0	0.0	0.0
30	0.6535009801079698	0.0	0.0	0.0	0.0
31	0.6747964446332272	0.0	0.0	0.0	0.0
32	0.701028324115822	0.0	0.0	0.0	0.0
33	0.72324219142384	0.0	0.0	0.0	0.0
34	0.7499906724717376	0.0	0.0	0.0	0.0
35	0.7788055597808461	0.0	0.0	0.0	0.0
36	0.8052670399591311	0.0	0.0	0.0	0.0
37	0.8310397180503457	0.0	0.0	0.0	0.0
38	0.8541145879672015	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACG	30	2.5284717E-6	44.0	21
TCGCAAT	30	2.5284717E-6	44.0	21
ACACGCT	25	4.4437897E-5	44.0	24
CGTATCT	25	4.4437897E-5	44.0	21
AATCACG	25	4.4437897E-5	44.0	31
CGACGTT	20	7.857602E-4	44.0	15
CGGTTAT	20	7.857602E-4	44.0	16
CGTCTAT	20	7.857602E-4	44.0	11
CGATTTA	30	2.5284717E-6	44.0	38
ATCGAGG	25	4.4437897E-5	44.0	26
CGATTAG	70	0.0	43.999996	32
GGCGATA	60	3.6379788E-12	40.333332	31
TAATCGG	60	3.6379788E-12	40.333332	40
TTGGGAT	18530	0.0	40.224503	5
TACCACG	55	7.8216544E-11	40.0	20
CACGTTA	55	7.8216544E-11	40.0	28
TTGGGAC	10230	0.0	39.978493	5
AAAGTTG	191330	0.0	39.83986	1
AAGTTGG	189885	0.0	39.836006	2
AGTTGGG	184510	0.0	39.814865	3
>>END_MODULE