FastQCFastQC Report
Fri 17 Jun 2016
SRR1527809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210891
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC448652.029272361233548No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT219460.9926314775355275No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT116580.5272987225512249No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA89900.4066233930121385No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC82850.37473579656346695No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA82820.3746001046636854No Hit
AAAGTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG76020.3438432740465269No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC50940.230404845829125No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49140.22226333184223016No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT34860.15767398754619744No Hit
AAAGTTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT30020.13578236104810235No Hit
AAAGTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA29740.13451590331680757No Hit
AAAGTTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC28020.1267362343959969No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT27450.12415808830014685No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT25390.11484057784847829No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC24970.11294089125153614No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG24410.11040797578894662No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC23980.10846305855874397No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT23020.10412091776573337No Hit
AAAGTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA23020.10412091776573337No Hit
AAAGTTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA22780.10303538256748071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC750.046.00000422
CAACGAA253.4181867E-546.040
CGTTCGG253.4181867E-546.028
CGTACTC301.8621886E-646.038
ATACGCA206.3127273E-446.012
TCGAACG206.3127273E-446.026
TACCGCG206.3127273E-446.029
AATCACG650.042.4615446
TTACGTC601.8189894E-1242.16666846
TGGGATA36200.041.9337046
TTGGGAT232750.041.622345
TTGGGAC128200.041.5507055
GTTGGGA722400.041.520354
AAAGTTG2438900.041.5158041
AAGTTGG2421150.041.5085832
TTGGGAG222100.041.4642075
AGTTGGG2353700.041.442413
ATACTCG451.5896148E-840.88888542
TGGGATT90550.040.8691336
TTGGGGG497150.040.8647275
Produced by FastQC (version 0.11.5)