FastQCFastQC Report
Fri 17 Jun 2016
SRR1527808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231039
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC425611.9076761992954852No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT214010.9592391706285727No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT109710.49174398116751883No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA87280.3912078632421935No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC82650.37045520046937774No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA77800.3487164500486096No Hit
AAAGTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG73480.3293532744160904No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC51870.23249257408767843No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47430.21259153246536702No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT33170.14867512401172728No Hit
AAAGTTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT29030.13011874736389636No Hit
AAAGTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA28950.12976017003736826No Hit
AAAGTTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC26200.11743407443796365No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT25880.11599976513185113No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC24660.1105314609022971No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT24650.11048663873648107No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC23740.10640782164722355No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG22980.10300133704520628No Hit
AAAGTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA22320.10004307410134919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG253.4181983E-546.028
TTATCGC253.4181983E-546.041
TAAGGCG206.312738E-446.042
AATCGCG206.312738E-446.019
TACCGCG206.312738E-446.029
ATCGCGT351.0201438E-745.99999645
TTGGGAT236650.042.2581865
TGGGATA38450.041.693116
TTGGGTA43100.041.6774945
AAAGTTG2459300.041.522141
AAGTTGG2440650.041.5058252
AGTTGGG2374500.041.4910553
GTTGGGA725600.041.4576874
TTGGGAG219050.041.254055
TTGGGGG494000.041.148585
TTGGGAC130450.041.1337665
TGGGATT86800.040.9919366
ACCGATA451.5896148E-840.8888921
TGGGGGG289500.040.8041466
GTTGGGG1297300.040.759274
Produced by FastQC (version 0.11.5)