##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527808_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2231039
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76346088078245	31.0	31.0	33.0	30.0	34.0
2	32.5657749595592	34.0	31.0	34.0	31.0	34.0
3	32.92665255963701	34.0	31.0	34.0	31.0	34.0
4	36.30854054994108	37.0	37.0	37.0	35.0	37.0
5	34.79663645503283	35.0	35.0	37.0	33.0	37.0
6	35.556801561962835	36.0	35.0	37.0	35.0	37.0
7	35.949023750817446	36.0	35.0	37.0	35.0	37.0
8	36.52204779925407	37.0	37.0	37.0	35.0	37.0
9	38.62711992035997	39.0	39.0	39.0	38.0	39.0
10	38.00243339538215	39.0	38.0	39.0	37.0	39.0
11	37.72072563500683	39.0	38.0	39.0	35.0	39.0
12	37.22447433684485	39.0	37.0	39.0	35.0	39.0
13	36.932920043083065	39.0	37.0	39.0	33.0	39.0
14	37.825112873419066	40.0	37.0	40.0	34.0	40.0
15	37.91213107435594	40.0	37.0	40.0	34.0	40.0
16	37.95129130418608	40.0	37.0	40.0	34.0	40.0
17	37.83652952727407	40.0	37.0	40.0	33.0	40.0
18	37.858773871725234	40.0	37.0	40.0	34.0	40.0
19	37.841573813814996	40.0	37.0	40.0	34.0	40.0
20	37.85709528161543	40.0	37.0	40.0	33.0	40.0
21	37.89905644858741	40.0	37.0	40.0	34.0	40.0
22	37.839667078881185	40.0	37.0	40.0	34.0	40.0
23	37.85904683871506	40.0	37.0	40.0	34.0	40.0
24	37.8435043044967	40.0	37.0	40.0	34.0	40.0
25	37.7647212800852	40.0	36.0	40.0	34.0	40.0
26	37.553487411022395	40.0	36.0	40.0	34.0	40.0
27	37.47181873557567	40.0	36.0	40.0	34.0	40.0
28	37.402465398408545	40.0	35.0	40.0	33.0	40.0
29	37.337019657657265	40.0	35.0	40.0	33.0	40.0
30	37.16782539435662	39.0	35.0	40.0	33.0	40.0
31	37.1076507403053	39.0	35.0	40.0	33.0	40.0
32	37.04260033105652	39.0	35.0	40.0	33.0	40.0
33	36.716253279301704	39.0	35.0	40.0	31.0	40.0
34	36.41167904281369	39.0	35.0	40.0	30.0	40.0
35	36.556116231047504	39.0	35.0	40.0	31.0	40.0
36	36.4902765034587	39.0	35.0	40.0	31.0	40.0
37	36.519812069623164	39.0	35.0	40.0	31.0	40.0
38	36.18476010504523	39.0	35.0	40.0	30.0	40.0
39	36.266904343671264	38.0	35.0	40.0	31.0	40.0
40	36.17841328636568	38.0	35.0	40.0	31.0	40.0
41	35.835353393643054	38.0	35.0	40.0	30.0	40.0
42	35.91213421190755	38.0	35.0	40.0	30.0	40.0
43	35.938089383466625	38.0	35.0	40.0	30.0	40.0
44	35.80993205407884	38.0	35.0	40.0	30.0	40.0
45	35.660676034798136	38.0	35.0	40.0	30.0	40.0
46	35.34090484299019	38.0	34.0	40.0	28.0	40.0
47	35.065346235543174	37.0	34.0	40.0	27.0	40.0
48	35.084159891422786	37.0	34.0	40.0	28.0	40.0
49	35.0790004119157	37.0	34.0	40.0	28.0	40.0
50	34.93727989515199	37.0	34.0	40.0	28.0	40.0
51	35.222342146416985	37.0	34.0	40.0	29.0	40.0
52	34.98691192758172	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	11.0
15	16.0
16	47.0
17	136.0
18	246.0
19	527.0
20	999.0
21	1689.0
22	2963.0
23	4522.0
24	6853.0
25	9939.0
26	13520.0
27	18474.0
28	23499.0
29	28536.0
30	36113.0
31	47301.0
32	63736.0
33	91299.0
34	193015.0
35	164584.0
36	204124.0
37	344581.0
38	754294.0
39	220010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.5424553313501	0.277045806908799	0.1610908639427639	0.019407997798335214
8	99.17173119788583	0.7089970188777516	0.06086850117815063	0.05840328205826971
9	97.01901221807418	1.5828947857926285	0.6527003786128347	0.7453926175203571
10	53.11368380382414	30.991434932334215	7.33353383782175	8.561347426019896
11	34.48093018544275	23.39434675951429	25.63325876419014	16.49146429085283
12	34.20836659511555	18.403667528895728	24.45569082387175	22.932275052116974
13	25.141604427354252	18.546605415683008	28.789501214456582	27.52228894250616
14	24.755506291015084	20.21215227524037	31.666187816528534	23.366153617216014
15	28.27368773024586	21.031142889030626	28.578657746458042	22.11651163426547
16	32.185228496678	22.391988665370707	25.502646973002264	19.92013586494902
17	30.159804467783847	22.308305681792206	25.31421458791173	22.21767526251222
18	29.87097043126543	23.41729570841209	25.07746390807153	21.634269952250946
19	28.269877846151502	25.985874742664738	24.336956906625122	21.407290504558638
20	29.36609355551382	25.205117436315543	23.58273432243901	21.846054685731627
21	26.907552938339492	27.00566865931075	26.17627930305118	19.91049909929858
22	26.67824273802475	24.308046609673788	27.220635766564367	21.79307488573709
23	30.94840565315084	23.81047574695019	25.36540150127362	19.875717098625348
24	32.18155307908109	22.98619611759364	23.10008924093214	21.732161562393127
25	28.616801409567472	22.204766478757207	22.90148222420137	26.276949887473954
26	25.635410228149308	22.40243223000584	27.18683985353909	24.775317688305762
27	24.464610434869137	22.005083730046852	25.00332804581184	28.526977789272173
28	26.56376692653064	27.246498156240207	21.50011720996361	24.689617707265537
29	30.5833739347452	23.918048944908627	22.994039996611445	22.50453712373473
30	30.2008167495055	21.616206619427093	26.563139416209218	21.61983721485819
31	32.24112173745058	22.664866010858617	22.807490142485182	22.286522109205624
32	34.13319982304209	22.20776956386688	22.67970214774372	20.97932846534731
33	33.351904650703105	21.94291538606004	23.75991634390972	20.94526361932714
34	27.86719550846041	23.531009543087325	28.146751356654903	20.455043591797363
35	25.287814332246093	28.678521531896124	26.34655871098623	19.68710542487155
36	26.55484731553326	30.34989527300957	22.444699532370343	20.65055787908683
37	29.649504109968493	28.714872308372914	21.822523048678217	19.813100532980375
38	28.72908093493659	28.978068066044564	21.20966957547582	21.08318142354302
39	29.59217655988981	25.349175877248225	21.3086817397634	23.749965823098567
40	26.974158676742093	24.669268443985068	22.29857927181013	26.05799360746271
41	24.22342236061315	27.659758525063882	22.579972828803083	25.536846285519886
42	22.58378271289744	25.226273498580703	23.13204744515896	29.057896343362888
43	23.269606672048315	23.616709524127547	24.33485026483177	28.77883353899237
44	25.301798847980695	22.701889119822646	23.395288024996425	28.60102400720023
45	28.035457918933737	23.95484794304358	22.21045889381584	25.799235244206848
46	24.805124428573414	27.279890669773145	22.902647600512584	25.012337301140857
47	23.87479555489617	25.014847342426556	24.98790922077113	26.12244788190614
48	25.566025515466112	23.828987301432203	25.701971144386093	24.903016038715595
49	27.84680142301412	23.529664878112843	23.934588324094737	24.6889453747783
50	23.51684573868946	24.409255060086355	24.788764338050566	27.285134863173617
51	23.19080930454376	24.531888505758975	27.038747417682973	25.238554772014293
52	22.947245655499522	24.121944977205686	26.325223360057805	26.605586007236987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	41.5
2	83.0
3	66.0
4	49.0
5	72.5
6	96.0
7	1134.0
8	2172.0
9	3131.5
10	4091.0
11	4821.5
12	5552.0
13	5220.5
14	4899.5
15	4910.0
16	4994.5
17	5079.0
18	5459.5
19	5840.0
20	7581.0
21	9322.0
22	10263.0
23	11204.0
24	14076.0
25	16948.0
26	18251.0
27	19554.0
28	23349.0
29	27144.0
30	29029.0
31	30914.0
32	37086.5
33	43259.0
34	47056.0
35	50853.0
36	55186.5
37	59520.0
38	64892.0
39	74701.5
40	79139.0
41	84769.5
42	90400.0
43	96989.0
44	103578.0
45	111505.5
46	119433.0
47	121993.0
48	124553.0
49	130144.0
50	135735.0
51	140238.0
52	144741.0
53	159070.5
54	173400.0
55	173221.5
56	173043.0
57	179988.0
58	186933.0
59	192928.5
60	198924.0
61	157931.0
62	116938.0
63	94367.5
64	59489.0
65	47181.0
66	41006.0
67	34831.0
68	29401.0
69	23971.0
70	20560.0
71	17149.0
72	13755.5
73	10362.0
74	7316.5
75	4271.0
76	3091.0
77	1911.0
78	1236.5
79	562.0
80	405.5
81	249.0
82	208.5
83	168.0
84	91.5
85	15.0
86	9.0
87	3.0
88	5.0
89	4.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2231039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.271607653298402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.26702353513227	6.514882731828313
2	9.774241158551394	1.8124585826161868
3	4.172071657481627	1.1604543452884801
4	2.1162141117594384	0.7848282781842755
5	1.1790381372842194	0.546578950858753
6	0.7644393676422139	0.4252549134908485
7	0.49676733380549526	0.32240822698137706
8	0.3755831681315455	0.2785807820878798
9	0.3059705230060989	0.2553154778508355
>10	4.531145439430576	11.643330298411358
>50	3.585015292883008	23.90365545206706
>100	2.324267214844951	37.85708212111917
>500	0.07686871554752665	4.721099018068441
>1k	0.027308622628726575	4.511322408837659
>5k	0.002528576169326535	1.7492949792539143
>10k+	0.0015171457015959208	3.5134534330554725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	42561	1.9076761992954852	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	21401	0.9592391706285727	No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	10971	0.49174398116751883	No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	8728	0.3912078632421935	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	8265	0.37045520046937774	No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7780	0.3487164500486096	No Hit
AAAGTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	7348	0.3293532744160904	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC	5187	0.23249257408767843	No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4743	0.21259153246536702	No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3317	0.14867512401172728	No Hit
AAAGTTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	2903	0.13011874736389636	No Hit
AAAGTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	2895	0.12976017003736826	No Hit
AAAGTTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	2620	0.11743407443796365	No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2588	0.11599976513185113	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC	2466	0.1105314609022971	No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT	2465	0.11048663873648107	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	2374	0.10640782164722355	No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2298	0.10300133704520628	No Hit
AAAGTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	2232	0.10004307410134919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.012057162604508483	0.0	0.0	0.0	0.0
8	0.025862389675841615	0.0	0.0	0.0	0.0
9	0.042132835867055664	0.0	0.0	0.0	0.0
10	0.1062285329839595	0.0	0.0	0.0	0.0
11	0.16216659592234828	0.0	0.0	0.0	0.0
12	0.18789451910074184	0.0	0.0	0.0	0.0
13	0.20434425395521996	0.0	0.0	0.0	0.0
14	0.2197630789959297	0.0	0.0	0.0	0.0
15	0.24038127527129735	0.0	0.0	0.0	0.0
16	0.2640922009879702	0.0	0.0	0.0	0.0
17	0.2835001987863054	0.0	0.0	0.0	0.0
18	0.30178764243924017	0.0	0.0	0.0	0.0
19	0.32670876663294546	0.0	0.0	0.0	0.0
20	0.34660980825525683	0.0	0.0	0.0	0.0
21	0.36834855867602495	0.0	0.0	0.0	0.0
22	0.38901157711720863	0.0	0.0	0.0	0.0
23	0.4157255879435546	0.0	0.0	0.0	0.0
24	0.4406467121372598	0.0	0.0	0.0	0.0
25	0.46166830790497165	0.0	0.0	0.0	0.0
26	0.4853792336216444	0.0	0.0	0.0	0.0
27	0.5068490510475164	0.0	0.0	0.0	0.0
28	0.5317701752412217	0.0	0.0	0.0	0.0
29	0.5493404642411002	0.0	0.0	0.0	0.0
30	0.5698690161848359	0.0	0.0	0.0	0.0
31	0.5878427046770586	0.0	0.0	0.0	0.0
32	0.6091332334396664	0.0	0.0	0.0	0.0
33	0.6291687415594259	0.0	0.0	0.0	0.0
34	0.6498317600006096	0.0	0.0	0.0	0.0
35	0.6785179461228602	0.0	0.0	0.0	0.0
36	0.702049583176269	0.0	0.0	0.0	0.0
37	0.723653867099589	0.0	0.0	0.0	0.0
38	0.7458856613443333	0.0	0.0	0.0	0.0
39	0.7664142132880689	0.0	0.0	0.0	0.0
40	0.7878840307139409	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGG	25	3.4181983E-5	46.0	28
TTATCGC	25	3.4181983E-5	46.0	41
TAAGGCG	20	6.312738E-4	46.0	42
AATCGCG	20	6.312738E-4	46.0	19
TACCGCG	20	6.312738E-4	46.0	29
ATCGCGT	35	1.0201438E-7	45.999996	45
TTGGGAT	23665	0.0	42.258186	5
TGGGATA	3845	0.0	41.69311	6
TTGGGTA	4310	0.0	41.677494	5
AAAGTTG	245930	0.0	41.52214	1
AAGTTGG	244065	0.0	41.505825	2
AGTTGGG	237450	0.0	41.491055	3
GTTGGGA	72560	0.0	41.457687	4
TTGGGAG	21905	0.0	41.25405	5
TTGGGGG	49400	0.0	41.14858	5
TTGGGAC	13045	0.0	41.133766	5
TGGGATT	8680	0.0	40.991936	6
ACCGATA	45	1.5896148E-8	40.88889	21
TGGGGGG	28950	0.0	40.804146	6
GTTGGGG	129730	0.0	40.75927	4
>>END_MODULE