FastQCFastQC Report
Fri 17 Jun 2016
SRR1527807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341618
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC73320.5465042955595408No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT65220.48612943475713655No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC41900.3123094651383628No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37030.27601001179173207No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGA36100.26907808332923383No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC33840.2522327517967111No Hit
ATGCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG28560.21287728697736613No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA27670.20624350597562047No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG27050.201622220333955No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG24780.18470235193624415No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAA24760.18455327820586784No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT24340.1814227298679654No Hit
ATGCTTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG23460.1748634857314079No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC22170.1652482301221361No Hit
ATGCTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG22100.16472647206581903No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21980.16383202968356117No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAA19320.1440052235435124No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC19140.14266355997012561No Hit
ATGCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT16900.12596730216797924No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT16740.1247747123249688No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAA16040.11955713176179807No Hit
ATGCTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT15550.11590482536757854No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAA15190.11322149822080502No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC15140.11284881389486427No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA14830.11053817107403151No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC14780.11016548674809074No Hit
ATGCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT13700.10211550530777017No Hit
ATGCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC13440.10017754681287817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA207.856952E-444.00000444
GTACCGA207.856952E-444.00000434
CATATAG408.314601E-944.00000418
CAACCGT207.856952E-444.00000412
CCGTAAA207.856952E-444.00000430
ATAAGCG207.856952E-444.00000412
TAAGGTC408.314601E-944.00000444
ATACGCC207.856952E-444.00000412
ATTGCGA207.856952E-444.00000412
CGATACA207.856952E-444.00000426
ACGAATC207.856952E-444.00000420
TTAATCG302.528035E-644.029
TAACGGG600.044.023
TCGCTAC351.446333E-744.035
TTAACCG254.443241E-544.031
CGTTTAG302.528035E-644.013
TACCGTA302.528035E-644.012
TACGTAC302.528035E-644.026
TGGGATA8250.042.1333356
TTGGGAC54100.041.3567475
Produced by FastQC (version 0.11.5)