##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527807_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1341618
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.77189706757065	33.0	31.0	34.0	3.0	34.0
2	30.241367512958234	34.0	31.0	34.0	16.0	34.0
3	32.18669993992329	34.0	31.0	34.0	28.0	34.0
4	36.0746941379737	37.0	35.0	37.0	35.0	37.0
5	36.25118252736621	37.0	35.0	37.0	35.0	37.0
6	36.55004330591868	37.0	37.0	37.0	35.0	37.0
7	36.528969498024026	37.0	37.0	37.0	35.0	37.0
8	36.65586254805764	37.0	37.0	37.0	35.0	37.0
9	38.748429880934815	39.0	39.0	39.0	39.0	39.0
10	38.2753660132765	39.0	39.0	39.0	37.0	39.0
11	38.04142237209101	39.0	38.0	39.0	37.0	39.0
12	37.662252593510225	39.0	37.0	39.0	35.0	39.0
13	37.50103308095151	39.0	37.0	39.0	35.0	39.0
14	38.34583167488808	40.0	38.0	40.0	35.0	40.0
15	38.42349685230818	40.0	38.0	40.0	35.0	40.0
16	38.501435580023525	40.0	38.0	40.0	35.0	40.0
17	38.50669191975659	40.0	38.0	40.0	35.0	40.0
18	38.43421227204763	40.0	38.0	40.0	35.0	40.0
19	38.43549132465426	40.0	38.0	40.0	35.0	40.0
20	38.339257523378485	40.0	38.0	40.0	35.0	40.0
21	38.357249231897605	40.0	38.0	40.0	35.0	40.0
22	38.356375659837596	40.0	38.0	40.0	35.0	40.0
23	38.25194354875978	40.0	38.0	40.0	35.0	40.0
24	38.10769011745519	40.0	38.0	40.0	35.0	40.0
25	37.916072235166794	40.0	37.0	40.0	34.0	40.0
26	37.66367252079206	40.0	36.0	40.0	34.0	40.0
27	37.49305390953312	40.0	36.0	40.0	33.0	40.0
28	37.297086055792334	40.0	35.0	40.0	33.0	40.0
29	37.18340615585062	40.0	35.0	40.0	33.0	40.0
30	37.241313846415295	40.0	35.0	40.0	33.0	40.0
31	37.21789809021644	40.0	35.0	40.0	33.0	40.0
32	37.07481786916991	40.0	35.0	40.0	33.0	40.0
33	36.84609106317894	40.0	35.0	40.0	33.0	40.0
34	36.45578994915095	40.0	35.0	40.0	31.0	40.0
35	36.296465909073966	40.0	35.0	40.0	30.0	40.0
36	36.0756437376362	39.0	35.0	40.0	30.0	40.0
37	35.78013786338585	39.0	35.0	40.0	28.0	40.0
38	35.53520301605971	39.0	35.0	40.0	26.0	40.0
39	35.23278161145721	39.0	35.0	40.0	24.0	40.0
40	34.86125484303282	39.0	35.0	40.0	22.0	40.0
41	34.580954489280856	39.0	35.0	40.0	21.0	40.0
42	34.37777221235851	38.0	35.0	40.0	20.0	40.0
43	34.17764222006562	38.0	34.0	40.0	18.0	40.0
44	33.77415926142911	37.0	34.0	40.0	17.0	40.0
45	33.71466169952997	37.0	34.0	40.0	18.0	40.0
46	33.54039525408872	36.0	33.0	40.0	17.0	40.0
47	33.482930312503264	36.0	33.0	40.0	17.0	40.0
48	33.32823724786042	36.0	33.0	40.0	17.0	40.0
49	33.150982619493774	35.0	33.0	40.0	15.0	40.0
50	32.60625453743167	35.0	33.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	7.0
14	4.0
15	23.0
16	60.0
17	147.0
18	314.0
19	642.0
20	1157.0
21	1964.0
22	3215.0
23	5240.0
24	7944.0
25	11665.0
26	15800.0
27	20829.0
28	27530.0
29	31602.0
30	31483.0
31	28845.0
32	30862.0
33	40417.0
34	63232.0
35	90100.0
36	129328.0
37	245554.0
38	348406.0
39	205240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.47101186775967	0.24612072885128256	0.21638051964120936	0.06648688374783285
8	99.16764682644389	0.5295098902966419	0.15764546987294448	0.14519781338652285
9	97.91729091291262	0.9749421966610466	0.5920463201895025	0.5157205702368334
10	62.26012173360822	25.187199336920045	4.791975062946382	7.760703866525345
11	34.0885408514197	31.08522694239344	17.172921055024602	17.653311151162253
12	29.51577870899168	22.385731258823302	26.17473826379789	21.923751768387127
13	21.85905376940381	24.489236131298178	28.79374009591404	24.857970003383972
14	24.55184709805623	25.95157488942456	26.100499546070495	23.39607846644872
15	23.066923669777836	25.999725704336107	25.530068916785552	25.403281709100504
16	26.221025657079732	26.754262390635787	23.360897066079914	23.663814886204566
17	29.48998895363658	25.99361368139068	20.35668871467139	24.159708650301354
18	26.725789308133912	26.293475490042624	22.84599640136015	24.13473880046332
19	28.10255974502429	25.271351457717472	20.796456219281495	25.829632577976742
20	25.016435378773988	27.496575031044607	22.53279249383953	24.95419709634188
21	27.97182208348427	25.86280148298547	23.454888053082172	22.710488380448083
22	27.34906657483725	26.941871680314367	21.300250891088226	24.40881085376016
23	27.691488933511625	26.584243801141604	22.98709468716132	22.737172578185444
24	27.846972834294114	26.771033185303118	20.879862971427038	24.50213100897573
25	25.437270519626303	25.67586302509358	22.04383065820524	26.843035797074876
26	27.629176114214328	24.722014761280782	22.756775773729927	24.89203335077496
27	28.045091821964224	23.508181911691704	22.917328181345212	25.52939808499886
28	31.337236083594583	25.535882792270232	19.95575491682431	23.171126207310873
29	31.91012642943073	25.04863530453527	19.983184483213552	23.058053782820444
30	31.064878378197076	25.52820549515585	19.05363523745209	24.353280889194988
31	32.11622086167598	26.114810624186617	18.599333044130294	23.169635470007112
32	32.000017888847644	26.650059853102746	19.164620629717252	22.185301628332358
33	30.809142393736515	27.331699485248407	19.28656294116507	22.57259517985
34	28.944453637324486	29.204810907426705	20.465065316654965	21.385670138593845
35	29.217705785104254	29.55178001487756	21.217440433864184	20.013073766154
36	29.71628287634781	28.737837447022923	19.029634366861504	22.516245309767758
37	28.7926965798014	28.869916772136335	19.258909764180267	23.078476883881997
38	30.144571703718942	28.419341422073945	18.691087925176912	22.7449989490302
39	29.630938165707377	26.990693327012604	19.382790034122976	23.99557847315704
40	28.93372032873739	27.358085535525017	20.13747579415303	23.570718341584566
41	28.677537123085706	26.842588575883745	20.132407287320238	24.34746701371031
42	27.588628059551972	24.96179985659107	21.887899536231625	25.56167254762533
43	28.790684084441327	24.707032851377964	23.172691481479827	23.329591582700886
44	27.874476937548543	24.354473479037996	22.79300069021137	24.97804889320209
45	27.438063591871902	24.358200322297403	23.115894390206453	25.087841695624242
46	27.834674251538065	25.062126477134328	21.95632437847435	25.146874892853255
47	28.67142510014028	25.027243224226268	22.241353350953847	24.059978324679605
48	28.975535510107946	24.94361286148516	22.375892392618464	23.70495923578843
49	27.723092564351404	25.31696801921262	22.70392913631153	24.256010280124446
50	26.43338118600078	25.372497983777798	22.786292372344437	25.40782845787698
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	59.5
4	119.0
5	101.0
6	83.0
7	152.5
8	222.0
9	1508.0
10	2794.0
11	4057.0
12	5320.0
13	4665.5
14	4011.0
15	4005.0
16	3999.0
17	4339.0
18	4679.0
19	5223.5
20	5768.0
21	6918.5
22	8069.0
23	9628.0
24	11187.0
25	12924.5
26	14662.0
27	17034.0
28	19406.0
29	20219.5
30	21033.0
31	20924.5
32	20816.0
33	24610.5
34	28405.0
35	29763.5
36	31122.0
37	31940.5
38	32759.0
39	32338.5
40	31918.0
41	34209.0
42	36500.0
43	36699.5
44	36899.0
45	38688.5
46	40478.0
47	43600.5
48	46723.0
49	51076.5
50	55430.0
51	61780.5
52	68131.0
53	75202.5
54	82274.0
55	99388.5
56	116503.0
57	117503.5
58	118504.0
59	115213.0
60	111922.0
61	103339.0
62	94756.0
63	91256.5
64	87757.0
65	76992.0
66	66227.0
67	54252.5
68	42278.0
69	37147.5
70	32017.0
71	25978.5
72	19940.0
73	22902.5
74	25865.0
75	17038.5
76	8212.0
77	5603.5
78	2995.0
79	2149.0
80	1303.0
81	839.0
82	375.0
83	218.5
84	62.0
85	73.0
86	84.0
87	47.0
88	10.0
89	5.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1341618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.03468444856024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89808988906974	12.170019216196938
2	8.919014105762605	2.860271535563221
3	3.4332869593635076	1.651550190442521
4	1.7118351539103436	1.0979494608361964
5	0.9213975084709595	0.7387159150010723
6	0.6135888512987404	0.5903222167037913
7	0.45045604986403726	0.5056044432260328
8	0.34128404342044866	0.43779055548605
9	0.2832707053674522	0.4087940736679358
>10	4.237973943602431	18.52583678214143
>50	2.209381065649442	24.723104390438166
>100	0.9236131260037521	25.53024473981737
>500	0.03503196890019357	3.6682263000203212
>1k	0.020829819346061044	6.039926956378134
>5k	9.468099702755019E-4	1.0516432240808198
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	7332	0.5465042955595408	No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6522	0.48612943475713655	No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4190	0.3123094651383628	No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3703	0.27601001179173207	No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGA	3610	0.26907808332923383	No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3384	0.2522327517967111	No Hit
ATGCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2856	0.21287728697736613	No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2767	0.20624350597562047	No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2705	0.201622220333955	No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2478	0.18470235193624415	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAA	2476	0.18455327820586784	No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	2434	0.1814227298679654	No Hit
ATGCTTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	2346	0.1748634857314079	No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2217	0.1652482301221361	No Hit
ATGCTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2210	0.16472647206581903	No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2198	0.16383202968356117	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAA	1932	0.1440052235435124	No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1914	0.14266355997012561	No Hit
ATGCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1690	0.12596730216797924	No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	1674	0.1247747123249688	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAA	1604	0.11955713176179807	No Hit
ATGCTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1555	0.11590482536757854	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAA	1519	0.11322149822080502	No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	1514	0.11284881389486427	No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1483	0.11053817107403151	No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	1478	0.11016548674809074	No Hit
ATGCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	1370	0.10211550530777017	No Hit
ATGCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1344	0.10017754681287817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.01833606883628574	0.0	0.0	0.0	0.0
8	0.036821211402947786	0.0	0.0	0.0	0.0
9	0.05508274337404537	0.0	0.0	0.0	0.0
10	0.17635422303517095	0.0	0.0	0.0	0.0
11	0.32438443729884364	0.0	0.0	0.0	0.0
12	0.3886352150910319	0.0	0.0	0.0	0.0
13	0.44684850680297966	0.0	0.0	0.0	0.0
14	0.5096085472914048	0.0	0.0	0.0	0.0
15	0.5874995714130252	0.0	0.0	0.0	0.0
16	0.6702354917718755	0.0	0.0	0.0	0.0
17	0.7321010898780428	0.0	0.0	0.0	0.0
18	0.7882273493647223	0.0	0.0	0.0	0.0
19	0.843980924525461	0.0	0.0	0.0	0.0
20	0.9094988290258479	0.0	0.0	0.0	0.0
21	0.9784454293248898	0.0	0.0	0.0	0.0
22	1.052833220782667	0.0	0.0	0.0	0.0
23	1.138923300074984	0.0	0.0	0.0	0.0
24	1.230230959930472	0.0	0.0	0.0	0.0
25	1.3166191866835417	0.0	0.0	0.0	0.0
26	1.4120263741243781	0.0	0.0	0.0	0.0
27	1.498861822068577	0.0	0.0	0.0	0.0
28	1.5927782722056503	0.0	0.0	0.0	0.0
29	1.6597869140098	0.0	0.0	0.0	0.0
30	1.7288080511740302	0.0	0.0	0.0	0.0
31	1.7838162576828873	0.0	0.0	0.0	0.0
32	1.838526316730992	0.0	0.0	0.0	0.0
33	1.892416470262027	0.0	0.0	0.0	0.0
34	1.9525677204688667	0.0	0.0	0.0	0.0
35	2.024793942836187	0.0	0.0	0.0	0.0
36	2.072646610286982	0.0	0.0	0.0	0.0
37	2.123778899806055	0.0	0.0	0.0	0.0
38	2.1710352723353443	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.856952E-4	44.000004	44
GTACCGA	20	7.856952E-4	44.000004	34
CATATAG	40	8.314601E-9	44.000004	18
CAACCGT	20	7.856952E-4	44.000004	12
CCGTAAA	20	7.856952E-4	44.000004	30
ATAAGCG	20	7.856952E-4	44.000004	12
TAAGGTC	40	8.314601E-9	44.000004	44
ATACGCC	20	7.856952E-4	44.000004	12
ATTGCGA	20	7.856952E-4	44.000004	12
CGATACA	20	7.856952E-4	44.000004	26
ACGAATC	20	7.856952E-4	44.000004	20
TTAATCG	30	2.528035E-6	44.0	29
TAACGGG	60	0.0	44.0	23
TCGCTAC	35	1.446333E-7	44.0	35
TTAACCG	25	4.443241E-5	44.0	31
CGTTTAG	30	2.528035E-6	44.0	13
TACCGTA	30	2.528035E-6	44.0	12
TACGTAC	30	2.528035E-6	44.0	26
TGGGATA	825	0.0	42.133335	6
TTGGGAC	5410	0.0	41.356747	5
>>END_MODULE