##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527806_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1693212
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.108700505311795	33.0	31.0	34.0	31.0	34.0
2	32.52220218141615	34.0	31.0	34.0	31.0	34.0
3	32.74254493825936	34.0	31.0	34.0	31.0	34.0
4	36.199314675303505	37.0	37.0	37.0	35.0	37.0
5	36.18029933640914	37.0	37.0	37.0	35.0	37.0
6	36.42712725872484	37.0	37.0	37.0	35.0	37.0
7	36.436680108574706	37.0	36.0	37.0	35.0	37.0
8	36.565138919402884	37.0	37.0	37.0	35.0	37.0
9	38.67882580562859	39.0	39.0	39.0	38.0	39.0
10	38.03134397819056	39.0	38.0	39.0	35.0	39.0
11	37.67558876265937	39.0	38.0	39.0	35.0	39.0
12	37.27444998027418	39.0	37.0	39.0	34.0	39.0
13	37.09080138813096	39.0	37.0	39.0	33.0	39.0
14	37.95761782930903	40.0	38.0	40.0	33.0	40.0
15	38.0558264411072	40.0	38.0	40.0	34.0	40.0
16	38.12561333134894	40.0	38.0	40.0	34.0	40.0
17	38.1189839193202	40.0	38.0	40.0	34.0	40.0
18	38.099406926008086	40.0	38.0	40.0	34.0	40.0
19	38.0962933170802	40.0	38.0	40.0	34.0	40.0
20	38.07762347538288	40.0	38.0	40.0	34.0	40.0
21	38.07767426642381	40.0	38.0	40.0	34.0	40.0
22	38.03959929412265	40.0	38.0	40.0	34.0	40.0
23	37.96540716697023	40.0	38.0	40.0	34.0	40.0
24	37.819437849483705	40.0	37.0	40.0	34.0	40.0
25	37.660814475682905	40.0	37.0	40.0	33.0	40.0
26	37.395003106521806	40.0	36.0	40.0	33.0	40.0
27	37.23506507159174	40.0	35.0	40.0	33.0	40.0
28	37.09607420689199	40.0	35.0	40.0	33.0	40.0
29	36.99987006943017	40.0	35.0	40.0	32.0	40.0
30	36.78882325426468	39.0	35.0	40.0	32.0	40.0
31	36.620593286605576	39.0	35.0	40.0	31.0	40.0
32	36.37000033073236	39.0	35.0	40.0	31.0	40.0
33	36.0934968568614	39.0	35.0	40.0	30.0	40.0
34	35.864405047920755	39.0	35.0	40.0	29.0	40.0
35	35.61372114064866	38.0	35.0	40.0	28.0	40.0
36	35.40884661814351	38.0	35.0	40.0	27.0	40.0
37	35.09897933631465	38.0	35.0	40.0	25.0	40.0
38	34.888579221030795	38.0	34.0	40.0	24.0	40.0
39	34.611194581659	38.0	34.0	40.0	23.0	40.0
40	34.22392647819647	38.0	33.0	40.0	21.0	40.0
41	33.94261262027437	37.0	33.0	40.0	20.0	40.0
42	33.76307337769872	37.0	33.0	40.0	19.0	40.0
43	33.523295960576704	37.0	33.0	40.0	18.0	40.0
44	33.318523020153414	36.0	33.0	40.0	18.0	40.0
45	33.17179715239438	36.0	33.0	40.0	18.0	40.0
46	33.06781962329584	35.0	33.0	40.0	18.0	40.0
47	32.875943473115	35.0	32.0	39.0	17.0	40.0
48	32.59870411974401	35.0	32.0	39.0	15.0	40.0
49	32.76344190804223	35.0	32.0	39.0	17.0	40.0
50	32.74124149840658	35.0	33.0	39.0	15.0	40.0
51	32.678917347620974	35.0	33.0	39.0	15.0	40.0
52	32.19220274838591	35.0	32.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	4.0
13	2.0
14	11.0
15	19.0
16	93.0
17	259.0
18	601.0
19	1249.0
20	2100.0
21	3815.0
22	5808.0
23	9294.0
24	13640.0
25	18557.0
26	25486.0
27	32919.0
28	39239.0
29	41103.0
30	38110.0
31	38722.0
32	45944.0
33	59934.0
34	93270.0
35	118807.0
36	177786.0
37	272954.0
38	462846.0
39	190635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.57235124721535	0.2080070304250147	0.17652839691662947	0.04311332544300418
8	99.25024155274119	0.514465997169876	0.1307574007271387	0.10453504936180466
9	98.0161964361226	0.9556393410866448	0.5400977550359908	0.4880664677547762
10	62.134747450407865	25.309352874891033	4.73325254014264	7.822647134558461
11	33.906563383675525	30.95950182257154	17.315433625558995	17.818501168193944
12	29.520284524324186	22.05884437388821	26.33739897898196	22.08347212280565
13	21.938717656147016	24.050857187404766	28.895968136299533	25.114457020148688
14	24.717341951273674	25.560355112059213	26.237470558914065	23.48483237775305
15	23.317989714223618	25.39085477778329	25.715090608854652	25.57606489913844
16	26.30338079342693	26.082912240168387	23.702938557014715	23.910768409389966
17	29.803828463299336	25.40036333312072	20.41008450211787	24.385723701462073
18	26.97210981259287	25.766236005887038	22.870615138565046	24.391039042955047
19	28.260666709189397	24.632828021535403	21.030502973047675	26.076002296227525
20	25.17894983026343	27.001048894054613	22.642764166566266	25.17723710911569
21	28.242594548113292	25.266298608797953	23.630177437910906	22.860929405177853
22	27.593945707920803	26.287552887647852	21.416337706087603	24.70216369834374
23	27.891663890877222	26.195125005020042	22.980524588769747	22.93268651533299
24	28.032992915240385	26.330548094391016	21.026545996602906	24.609912993765697
25	25.594609535013923	25.201982976732978	22.130010890544124	27.07339659770897
26	27.919185547940838	24.21250262814107	22.69839807419272	25.169913749725374
27	28.147390876039147	22.960030994346837	22.965582573239498	25.92699555637451
28	31.432153799996694	25.08599041348632	20.07787565880705	23.40398012770994
29	32.023101655315465	24.672752142082622	19.96767091185274	23.336475290749178
30	31.011710287902517	25.176764634316317	19.2283659695301	24.583159108251063
31	32.312138113833356	25.741076722820296	18.68927222344278	23.25751293990357
32	32.26949726318972	26.18419902528449	19.246438130606208	22.29986558091958
33	31.116835930763543	26.789734540033972	19.333550671741044	22.75987885746144
34	28.930399737304015	28.820254049699624	20.49761045870216	21.751735754294206
35	29.287590685631805	29.17590945492945	21.306310137183058	20.23018972225569
36	29.919939145245838	28.330829216896642	19.117334391676884	22.631897246180632
37	28.917938214470485	28.44812108584158	19.356465699510753	23.277475000177176
38	30.221200889197576	28.160561111071736	18.787665100412706	22.830572899317982
39	29.70029742288621	26.709472883490076	19.404067535547824	24.186162158075895
40	28.824329144844235	27.236636640893163	20.235327885698897	23.7037063285637
41	28.700068272608508	26.697070420006476	20.14851064131367	24.45435066607135
42	27.6214083056345	24.62792609549188	21.972913019751807	25.77775257912181
43	28.910851092479856	24.373557475378156	23.236428751981443	23.47916268016055
44	27.78872344396331	23.931262003812872	22.963810792741842	25.31620375948198
45	27.56193554026312	23.93551427700725	23.295842458002898	25.206707724726733
46	27.696059323935813	24.622020160499687	22.228462826863975	25.45345768870053
47	28.837145023777293	24.617000115756326	22.38349362040902	24.162361240057358
48	29.3543868103935	24.501775324058652	22.285277921488863	23.85855994405898
49	27.812583421331766	24.950744502165115	22.86606756862106	24.37060450788206
50	26.35919187910315	25.224189292303624	22.878824388204194	25.537794440389032
51	26.535661216669855	25.642624786500452	22.413968244968736	25.40774575186096
52	27.492068329305486	25.541692357483882	22.11790372381013	24.8483355894005
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	82.5
4	165.0
5	127.0
6	89.0
7	168.0
8	247.0
9	1725.0
10	3203.0
11	4715.0
12	6227.0
13	5385.0
14	4433.5
15	4324.0
16	4571.5
17	4819.0
18	4937.0
19	5055.0
20	6036.0
21	7017.0
22	8315.5
23	9614.0
24	11634.0
25	13654.0
26	15724.0
27	17794.0
28	20229.0
29	22664.0
30	23830.0
31	24996.0
32	28891.0
33	32786.0
34	36196.5
35	39607.0
36	39258.0
37	38909.0
38	37771.0
39	38308.0
40	39983.0
41	41019.5
42	42056.0
43	44684.5
44	47313.0
45	47759.0
46	48205.0
47	51067.0
48	53929.0
49	58631.5
50	63334.0
51	73120.0
52	82906.0
53	91309.5
54	99713.0
55	116455.5
56	133198.0
57	146126.0
58	159054.0
59	144881.0
60	130708.0
61	129301.5
62	127895.0
63	117713.5
64	98828.5
65	90125.0
66	77105.0
67	64085.0
68	52465.0
69	40845.0
70	36080.5
71	31316.0
72	32869.0
73	34422.0
74	25173.0
75	15924.0
76	10558.5
77	5193.0
78	3687.0
79	2181.0
80	1458.0
81	735.0
82	414.0
83	93.0
84	93.0
85	93.0
86	57.0
87	21.0
88	13.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1693212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.265648885018464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30849618662012	12.737385036853702
2	8.146683669251773	2.650221922827265
3	3.355540950602803	1.6374015276541882
4	1.6465142591649413	1.071264912950129
5	0.9382327029885134	0.7630481859626486
6	0.6097007728054267	0.5950307217226489
7	0.41347544437739897	0.4707812480573827
8	0.2957773159793562	0.3848807975898695
9	0.24185610014929368	0.35405517651554447
>10	2.885474434649802	12.35811029164244
>50	1.9460499564033624	22.90783342977769
>100	1.1465072263280327	31.621178476048193
>500	0.046092566553865136	5.040988993567988
>1k	0.01850961334052852	6.025785911203143
>5k	0.0010888007847369718	1.38203336762718
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9370	0.5533861087684235	No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8613	0.5086781808775275	No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5428	0.320574151376201	No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4436	0.2619872762536528	No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4296	0.25371896726458354	No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG	3894	0.2299771085959703	No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3726	0.2200551378090871	No Hit
ATGCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3639	0.21491697436587975	No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3365	0.1987347124872727	No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3299	0.19483679539242577	No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3115	0.18396987500679182	No Hit
ATGCTTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	2961	0.1748747351188156	No Hit
ATGCTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2824	0.16678360417951207	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAA	2780	0.16418499278294743	No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2587	0.1527865382480162	No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2517	0.14865238375348153	No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2474	0.14611283170683884	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAA	2354	0.139025709716208	No Hit
ATGCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2270	0.13406472432276642	No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1981	0.11699657219533054	No Hit
ATGCTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1955	0.11546102909736052	No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1891	0.11168123070235741	No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1848	0.10914167865571471	No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1846	0.10902355995587086	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAA	1831	0.108137669707042	No Hit
ATGCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1825	0.10778331360751045	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAA	1763	0.1041216339123512	No Hit
ATGCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1724	0.10181831926539618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.01582790577907551	0.0	0.0	0.0	0.0
8	0.03431348230463758	0.0	0.0	0.0	0.0
9	0.05014138808371309	0.0	0.0	0.0	0.0
10	0.15751128624177008	0.0	0.0	0.0	0.0
11	0.2894498739673473	0.0	0.0	0.0	0.0
12	0.34644214664200346	0.0	0.0	0.0	0.0
13	0.39859155262306195	0.0	0.0	0.0	0.0
14	0.4577690212448294	0.0	0.0	0.0	0.0
15	0.5292308346503568	0.0	0.0	0.0	0.0
16	0.600928885455572	0.0	0.0	0.0	0.0
17	0.6579211581302282	0.0	0.0	0.0	0.0
18	0.7078853681641756	0.0	0.0	0.0	0.0
19	0.7613340798435163	0.0	0.0	0.0	0.0
20	0.8214564980640345	0.0	0.0	0.0	0.0
21	0.8861264862285407	0.0	0.0	0.0	0.0
22	0.9529816703401582	0.0	0.0	0.0	0.0
23	1.028105163440845	0.0	0.0	0.0	0.0
24	1.108484938684583	0.0	0.0	0.0	0.0
25	1.1804192268894858	0.0	0.0	0.0	0.0
26	1.2569010850383768	0.0	0.0	0.0	0.0
27	1.3246421593988231	0.0	0.0	0.0	0.0
28	1.395808676054741	0.0	0.0	0.0	0.0
29	1.4406937819954029	0.0	0.0	0.0	0.0
30	1.4903626952797404	0.0	0.0	0.0	0.0
31	1.5298734003775074	0.0	0.0	0.0	0.0
32	1.5755853372170763	0.0	0.0	0.0	0.0
33	1.6233643513039124	0.0	0.0	0.0	0.0
34	1.6688991100937154	0.0	0.0	0.0	5.905934992192354E-5
35	1.7296711811633747	0.0	0.0	0.0	5.905934992192354E-5
36	1.7736703968552077	0.0	0.0	0.0	5.905934992192354E-5
37	1.8160159507492268	0.0	0.0	0.0	5.905934992192354E-5
38	1.8579480891937927	0.0	0.0	0.0	1.1811869984384708E-4
39	1.8967500820924963	0.0	0.0	0.0	1.1811869984384708E-4
40	1.9385050424872963	0.0	0.0	0.0	1.1811869984384708E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	20	6.312304E-4	46.000004	45
CGAACAC	20	6.312304E-4	46.000004	39
CACGGTA	20	6.312304E-4	46.000004	21
CGAAATT	20	6.312304E-4	46.000004	45
ACGGGTA	20	6.312304E-4	46.000004	15
TCGATAC	20	6.312304E-4	46.000004	25
CGTTAAG	20	6.312304E-4	46.000004	12
ATACGTA	20	6.312304E-4	46.000004	26
GCGTAAT	20	6.312304E-4	46.000004	20
TACAATC	20	6.312304E-4	46.000004	37
ACGGTAC	20	6.312304E-4	46.000004	22
AGCGTTA	25	3.417846E-5	46.0	16
GCGAATA	50	1.6370905E-11	46.0	39
ATTACCG	25	3.417846E-5	46.0	26
CGACATA	30	1.8619303E-6	46.0	19
CGCGAAA	25	3.417846E-5	46.0	36
ATACGGT	25	3.417846E-5	46.0	43
ATTGCGA	25	3.417846E-5	46.0	12
ATTTGCG	45	3.110472E-10	46.0	16
ACGACTA	25	3.417846E-5	46.0	12
>>END_MODULE