FastQCFastQC Report
Fri 17 Jun 2016
SRR1527805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704251
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT89450.5248640018401045No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG80510.47240694005753847No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC51080.29972110915586964No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC43310.2541292333112904No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38990.228780854463339No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC35200.2065423461684928No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG35090.20589690133671623No Hit
ATGCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT34700.20360850602405395No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC32710.19193182224918748No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG31910.18723767801808536No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT30060.17638246948366174No Hit
ATGCTTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT28430.16681815061279118No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAA26770.1570778013332543No Hit
ATGCTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG26610.1561389724870339No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA25880.15185556587615323No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG24700.14493170313527762No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22940.13460458582685297No Hit
ATGCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG21980.12897161274953045No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAA20880.12251716443176504No Hit
ATGCTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC18960.11125121827711998No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG18710.10978429820490056No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC18580.10902149976734649No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT17900.10503147717090969No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG17880.10491412356513213No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAA17660.10362323390157904No Hit
ATGCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT17350.10180425301202697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA206.312315E-446.00000417
CGAAATT206.312315E-446.00000445
GATCGAC206.312315E-446.0000049
CGACGTC206.312315E-446.00000439
TCGGCTA206.312315E-446.00000444
CGTTACC206.312315E-446.00000439
TTGCGAT206.312315E-446.00000419
CGCTTAA206.312315E-446.00000411
ACGTTAG253.4178553E-546.028
CGGTTAG253.4178553E-546.037
TGTATCG253.4178553E-546.046
CGACATA253.4178553E-546.019
CGCGAAA453.110472E-1046.036
TATTGCG253.4178553E-546.015
ATACGGA501.6370905E-1146.034
TACGTTT253.4178553E-546.027
TACCGTA253.4178553E-546.012
CGAGTAT351.0199619E-745.99999645
ACCGTTA351.0199619E-745.99999629
TTGGGAT89000.042.382025
Produced by FastQC (version 0.11.5)