##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527805_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1704251
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07816571620025	33.0	31.0	34.0	31.0	34.0
2	32.505740058242594	34.0	31.0	34.0	31.0	34.0
3	32.73293077134765	34.0	31.0	34.0	31.0	34.0
4	36.186640934932704	37.0	37.0	37.0	35.0	37.0
5	36.191100958720284	37.0	37.0	37.0	35.0	37.0
6	36.37061970331835	37.0	37.0	37.0	35.0	37.0
7	36.39088315042796	37.0	36.0	37.0	35.0	37.0
8	36.53102917351963	37.0	37.0	37.0	35.0	37.0
9	38.632349929675854	39.0	39.0	39.0	38.0	39.0
10	37.93724985345469	39.0	38.0	39.0	35.0	39.0
11	37.63622670604271	39.0	37.0	39.0	35.0	39.0
12	37.21801190082916	39.0	37.0	39.0	34.0	39.0
13	36.88156498074521	39.0	37.0	39.0	33.0	39.0
14	37.88989063230709	40.0	38.0	40.0	33.0	40.0
15	38.000920639037325	40.0	38.0	40.0	34.0	40.0
16	38.10361853975735	40.0	38.0	40.0	34.0	40.0
17	38.129788980613775	40.0	38.0	40.0	34.0	40.0
18	38.11982507271523	40.0	38.0	40.0	34.0	40.0
19	38.074124058017276	40.0	38.0	40.0	34.0	40.0
20	38.06444062523654	40.0	38.0	40.0	34.0	40.0
21	38.123047602729876	40.0	38.0	40.0	34.0	40.0
22	37.97966262011875	40.0	38.0	40.0	34.0	40.0
23	37.92535489197307	40.0	38.0	40.0	34.0	40.0
24	37.827125229793026	40.0	37.0	40.0	34.0	40.0
25	37.642814350703034	40.0	37.0	40.0	33.0	40.0
26	37.336194023063506	40.0	35.0	40.0	33.0	40.0
27	37.1698306176731	40.0	35.0	40.0	33.0	40.0
28	37.04644092918238	40.0	35.0	40.0	32.0	40.0
29	36.953420593562804	40.0	35.0	40.0	32.0	40.0
30	36.731378183143214	39.0	35.0	40.0	31.0	40.0
31	36.585597426670134	39.0	35.0	40.0	31.0	40.0
32	36.33167766954516	39.0	35.0	40.0	31.0	40.0
33	35.87605581572198	39.0	35.0	40.0	30.0	40.0
34	35.765265210347536	38.0	35.0	40.0	29.0	40.0
35	35.59908076920595	38.0	35.0	40.0	28.0	40.0
36	35.254497723633435	38.0	35.0	40.0	26.0	40.0
37	35.02530085063761	38.0	34.0	40.0	25.0	40.0
38	34.54255857851924	38.0	34.0	40.0	23.0	40.0
39	34.42869917635372	38.0	34.0	40.0	23.0	40.0
40	34.11606036904188	38.0	33.0	40.0	21.0	40.0
41	33.64516083605056	37.0	33.0	40.0	18.0	40.0
42	33.59131724141573	37.0	33.0	40.0	18.0	40.0
43	33.46906940350922	37.0	33.0	40.0	18.0	40.0
44	33.25611544308907	36.0	33.0	40.0	18.0	40.0
45	33.10232060887745	36.0	33.0	40.0	18.0	40.0
46	32.84899348746165	35.0	32.0	40.0	15.0	40.0
47	32.58781540981933	35.0	32.0	39.0	15.0	40.0
48	32.46540679747291	35.0	31.0	39.0	15.0	40.0
49	32.31541245978438	35.0	31.0	39.0	15.0	40.0
50	32.09303735189241	35.0	31.0	39.0	14.0	40.0
51	32.28915987140392	35.0	32.0	39.0	14.0	40.0
52	32.004501684317624	35.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	10.0
15	31.0
16	92.0
17	249.0
18	603.0
19	1219.0
20	2160.0
21	3772.0
22	6064.0
23	9398.0
24	14028.0
25	19498.0
26	26268.0
27	33460.0
28	40719.0
29	42020.0
30	39143.0
31	40274.0
32	48743.0
33	63360.0
34	97818.0
35	124601.0
36	189530.0
37	284333.0
38	451609.0
39	165239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.56526356739705	0.2062489621540489	0.18136999772920773	0.047117472719687414
8	99.23350492386392	0.5184095535223391	0.1327856049373009	0.11529991767644555
9	97.98122459661165	0.9603632328806027	0.5578990418664856	0.5005131286412623
10	62.154987733614355	25.258251278714226	4.781983405026606	7.80477758264481
11	33.974396963827516	30.882950926829437	17.32062941432923	17.82202269501382
12	29.562429477817524	22.087415527407643	26.25822135354475	22.091933641230078
13	22.15219471779685	24.032551543170577	28.82920414891938	24.986049590113193
14	24.79452850548423	25.549816312268558	26.18334975305868	23.47230542918854
15	23.302025347205312	25.419436456249695	25.751723191008836	25.526815005536157
16	26.329161608237285	26.114228479255697	23.690700489540568	23.865909422966453
17	29.777069222784675	25.429822250361013	20.4413551759688	24.35175335088552
18	27.046133462735245	25.764338703629925	22.87239379645369	24.31713403718114
19	28.276820726524438	24.631524347059205	21.067671370003595	26.023983556412762
20	25.13684897353735	27.001480415736882	22.730630640674406	25.13103997005136
21	28.24947733637827	25.24757210058847	23.675327167183706	22.827623395849557
22	27.746206398001235	26.29149110078269	21.328079021223985	24.63422347999209
23	27.915195590320906	26.13259431855988	22.98170868023548	22.970501410883728
24	27.995406779869867	26.352867036604348	21.056933515074952	24.594792668450832
25	25.57610351996273	25.20391655923922	22.13588256659377	27.084097354204278
26	27.98813085631166	24.220258635611774	22.73203888394374	25.059571624132833
27	28.135879045985597	23.035808692498932	22.888999331671215	25.939312929844256
28	31.411100829631316	25.14876036452377	20.072747500221507	23.367391305623407
29	32.03090389854546	24.605237139365034	20.079906070173937	23.28395289191557
30	30.959524154599293	25.149464486158436	19.282429642112575	24.608581717129695
31	32.32428791298934	25.716282402064017	18.689969963344602	23.26945972160204
32	32.24390069303172	26.119157330698357	19.320862948004724	22.316079028265204
33	31.055988818548443	26.759145219806236	19.44725278142715	22.737613180218172
34	28.90161132368413	28.81576716105785	20.555188173573026	21.727433341685
35	29.299792108087363	29.18103025904048	21.306309927352252	20.2128677055199
36	30.094099988792735	28.264498597917797	19.181798925158326	22.45960248813115
37	28.945266865033375	28.396286697205987	19.385935522408378	23.272510915352257
38	30.273240268012163	28.076674151870822	18.732275938227406	22.917809641889605
39	29.694510961120162	26.728574605501187	19.374200161830622	24.20271427154803
40	28.88119179627883	27.09219475300293	20.344831835216763	23.681781615501475
41	28.86458626106131	26.658338472443322	20.139580378711823	24.33749488778355
42	27.566303320344243	24.606058614605477	22.054087103366815	25.773550961683462
43	28.88289342356261	24.348056712303528	23.273801805015808	23.49524805911805
44	27.818789603174647	23.914787199772803	23.0072330894921	25.259190107560446
45	27.496184540892155	23.89378090433862	23.412044352621766	25.19799020214745
46	27.904281704983596	24.544271941163593	22.210871520685625	25.340574833167178
47	28.963221966717345	24.561464244410008	22.355333809397795	24.119979979474852
48	29.270218999431425	24.561640274818675	22.359558539205786	23.80858218654412
49	27.93684733058687	24.88935021895249	22.763548327095013	24.410254123365632
50	26.55671024983996	25.164779131712407	22.783967854500307	25.49454276394733
51	26.617792801647177	25.57234820457785	22.392124164809058	25.41773482896592
52	27.498238228993266	25.503520314789313	22.12772649099223	24.870514965225194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	74.5
4	149.0
5	121.0
6	93.0
7	172.5
8	252.0
9	1657.5
10	3063.0
11	4520.0
12	5977.0
13	5379.0
14	4678.0
15	4575.0
16	4765.0
17	4955.0
18	5131.5
19	5308.0
20	6226.0
21	7144.0
22	8317.5
23	9491.0
24	11421.0
25	13351.0
26	15565.0
27	17779.0
28	20070.0
29	22361.0
30	23749.0
31	25137.0
32	29146.0
33	33155.0
34	36366.0
35	39577.0
36	39455.0
37	39333.0
38	38345.5
39	38692.5
40	40027.0
41	41238.5
42	42450.0
43	45163.0
44	47876.0
45	48536.5
46	49197.0
47	51609.0
48	54021.0
49	59026.5
50	64032.0
51	73489.0
52	82946.0
53	91864.5
54	100783.0
55	117408.5
56	134034.0
57	146476.5
58	158919.0
59	145261.0
60	131603.0
61	130405.0
62	129207.0
63	118669.0
64	99425.0
65	90719.0
66	77651.0
67	64583.0
68	52834.5
69	41086.0
70	36641.5
71	32197.0
72	33298.0
73	34399.0
74	25125.0
75	15851.0
76	10513.0
77	5175.0
78	3714.5
79	2254.0
80	1482.0
81	710.0
82	405.5
83	101.0
84	96.0
85	91.0
86	50.5
87	10.0
88	9.0
89	5.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1704251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.932989678532646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32911477087814	13.263460919438788
2	8.286972551852866	2.8064644137361587
3	3.304848434853321	1.678828933094582
4	1.645736930818513	1.1146898585251954
5	0.9407844114194007	0.7965146364144563
6	0.5873963989114225	0.5967826295984617
7	0.4071986787910557	0.48265637175567544
8	0.28756396505498133	0.38954541217551375
9	0.2208507426711976	0.3365697011532892
>10	2.94842064511308	13.174591265163885
>50	1.9362075186669954	23.521439116475342
>100	1.0441044164905535	29.939791873769618
>500	0.042343229368940936	4.774222613272694
>1k	0.017371581279565514	5.769868581463096
>5k	0.0010857238299728446	1.3545736739632328
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	8945	0.5248640018401045	No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8051	0.47240694005753847	No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5108	0.29972110915586964	No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4331	0.2541292333112904	No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3899	0.228780854463339	No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3520	0.2065423461684928	No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG	3509	0.20589690133671623	No Hit
ATGCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3470	0.20360850602405395	No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3271	0.19193182224918748	No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3191	0.18723767801808536	No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3006	0.17638246948366174	No Hit
ATGCTTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	2843	0.16681815061279118	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAA	2677	0.1570778013332543	No Hit
ATGCTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2661	0.1561389724870339	No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2588	0.15185556587615323	No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2470	0.14493170313527762	No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2294	0.13460458582685297	No Hit
ATGCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2198	0.12897161274953045	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAA	2088	0.12251716443176504	No Hit
ATGCTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1896	0.11125121827711998	No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1871	0.10978429820490056	No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1858	0.10902149976734649	No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1790	0.10503147717090969	No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1788	0.10491412356513213	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAA	1766	0.10362323390157904	No Hit
ATGCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1735	0.10180425301202697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0161361207944135	0.0	0.0	0.0	0.0
8	0.03291768642060354	0.0	0.0	0.0	0.0
9	0.047469533537020075	0.0	0.0	0.0	0.0
10	0.15302910193392874	0.0	0.0	0.0	0.0
11	0.2856386764625633	0.0	0.0	0.0	0.0
12	0.34144031600978964	0.0	0.0	0.0	0.0
13	0.3957163586819078	0.0	0.0	0.0	0.0
14	0.4497576941424708	0.0	0.0	0.0	0.0
15	0.5195244127772259	0.0	0.0	0.0	0.0
16	0.5915795267246433	0.0	0.0	0.0	0.0
17	0.6495522079787543	0.0	0.0	0.0	0.0
18	0.7020679465642091	0.0	0.0	0.0	0.0
19	0.7547010387554416	0.0	0.0	0.0	0.0
20	0.8130257808268853	0.0	0.0	0.0	0.0
21	0.8783917392449822	0.0	0.0	0.0	5.867680288877636E-5
22	0.9463981537930739	0.0	0.0	0.0	5.867680288877636E-5
23	1.019098712572268	0.0	0.0	0.0	5.867680288877636E-5
24	1.0944984042843455	0.0	0.0	0.0	5.867680288877636E-5
25	1.167257639866428	0.0	0.0	0.0	5.867680288877636E-5
26	1.246001909343166	0.0	0.0	0.0	5.867680288877636E-5
27	1.3104290389150424	0.0	0.0	0.0	5.867680288877636E-5
28	1.3772619174053586	0.0	0.0	0.0	5.867680288877636E-5
29	1.424144682913491	0.0	0.0	0.0	5.867680288877636E-5
30	1.4701472963782918	0.0	0.0	0.0	5.867680288877636E-5
31	1.5077591270299973	0.0	0.0	0.0	5.867680288877636E-5
32	1.5542311549179082	0.0	0.0	0.0	5.867680288877636E-5
33	1.5954222705458292	0.0	0.0	0.0	5.867680288877636E-5
34	1.6404860551644094	0.0	0.0	0.0	5.867680288877636E-5
35	1.700453747716739	0.0	0.0	0.0	5.867680288877636E-5
36	1.7448134107006539	0.0	0.0	0.0	5.867680288877636E-5
37	1.791461468997231	0.0	0.0	0.0	5.867680288877636E-5
38	1.8317137557789316	0.0	0.0	0.0	1.1735360577755273E-4
39	1.8710858905173005	0.0	0.0	0.0	1.1735360577755273E-4
40	1.9080522763372296	0.0	0.0	0.0	1.1735360577755273E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	6.312315E-4	46.000004	17
CGAAATT	20	6.312315E-4	46.000004	45
GATCGAC	20	6.312315E-4	46.000004	9
CGACGTC	20	6.312315E-4	46.000004	39
TCGGCTA	20	6.312315E-4	46.000004	44
CGTTACC	20	6.312315E-4	46.000004	39
TTGCGAT	20	6.312315E-4	46.000004	19
CGCTTAA	20	6.312315E-4	46.000004	11
ACGTTAG	25	3.4178553E-5	46.0	28
CGGTTAG	25	3.4178553E-5	46.0	37
TGTATCG	25	3.4178553E-5	46.0	46
CGACATA	25	3.4178553E-5	46.0	19
CGCGAAA	45	3.110472E-10	46.0	36
TATTGCG	25	3.4178553E-5	46.0	15
ATACGGA	50	1.6370905E-11	46.0	34
TACGTTT	25	3.4178553E-5	46.0	27
TACCGTA	25	3.4178553E-5	46.0	12
CGAGTAT	35	1.0199619E-7	45.999996	45
ACCGTTA	35	1.0199619E-7	45.999996	29
TTGGGAT	8900	0.0	42.38202	5
>>END_MODULE