FastQCFastQC Report
Fri 17 Jun 2016
SRR1527804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369686
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC248871.8169857909039004No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG128840.9406535512518928No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT115640.844281098003484No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT80510.5877989553810143No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT70490.5146435022333586No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC49890.36424406761841766No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC41150.3004338220584864No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT40720.2972944163844852No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC32970.2407121048181846No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC27240.19887769897626173No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG24600.17960320832657994No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC23760.17347041584713577No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC23070.16843276488187803No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGTGGGGAAGAGCACCAACTGT22750.16609646298494693No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC22030.1608397837168519No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTA20570.15018040631210364No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT17930.1309059156624219No Hit
GTCGGTGGGAGAGGCCTTCACAGAGGGCCTCAATTCTACTCTCTGGTTTT16910.12345895336595394No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG15700.11462481181818314No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACC15270.11148540614418195No Hit
GTCGGTGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAGCACTGACTGC15070.11002521745860001No Hit
GTCGGTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTG15030.10973317972148361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG207.857012E-444.034
ATTACGA408.314601E-944.039
TCGATAG207.857012E-444.036
TCCCGTA207.857012E-444.013
ACCGTAA351.4463512E-744.029
ATACGTT254.4432876E-544.010
CGATATT254.4432876E-544.021
TACGTAC351.4463512E-744.027
GTCGGTA9350.042.823531
GTCGGTG1402600.042.616571
CGGTGGG1372300.042.5459483
TCGGTGG1397600.042.488842
GGTGGGA423050.042.1694834
GTGGGGG358250.041.973485
GGTGGGG794250.041.955814
GTGGGAT136350.041.9347275
TGGGGGG214450.041.6712536
GTGGGGC152800.041.537965
GTGGGAA84900.040.96825
TGGGGCT88900.040.8076486
Produced by FastQC (version 0.11.5)