##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527804_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1369686
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30069592592755	31.0	31.0	31.0	31.0	33.0
2	31.34847841038019	31.0	31.0	31.0	30.0	34.0
3	32.288149984741025	31.0	31.0	34.0	31.0	34.0
4	36.29165370749208	37.0	35.0	37.0	35.0	37.0
5	36.35628311890463	37.0	37.0	37.0	35.0	37.0
6	33.331055438983825	35.0	32.0	35.0	31.0	35.0
7	35.38271253411366	35.0	35.0	37.0	35.0	37.0
8	36.36474929290363	37.0	35.0	37.0	35.0	37.0
9	38.67173133112261	39.0	39.0	39.0	38.0	39.0
10	37.8926534986851	39.0	37.0	39.0	35.0	39.0
11	37.338784947790955	39.0	37.0	39.0	35.0	39.0
12	36.42145791079123	37.0	35.0	39.0	33.0	39.0
13	36.225360411072316	37.0	35.0	39.0	33.0	39.0
14	36.744756097382904	38.0	35.0	40.0	33.0	40.0
15	36.69907701473184	38.0	35.0	40.0	32.0	40.0
16	36.64949119725251	38.0	35.0	40.0	32.0	40.0
17	36.25665517498171	38.0	35.0	40.0	30.0	40.0
18	36.14158427552008	38.0	34.0	40.0	30.0	40.0
19	36.016243869032756	38.0	34.0	40.0	30.0	40.0
20	35.81473564013942	38.0	34.0	40.0	30.0	40.0
21	35.88732234979404	38.0	34.0	40.0	30.0	40.0
22	36.04264846103413	38.0	34.0	40.0	30.0	40.0
23	36.04437513415483	38.0	34.0	40.0	30.0	40.0
24	36.12764385413883	38.0	34.0	40.0	31.0	40.0
25	36.04451166179694	38.0	34.0	39.0	31.0	40.0
26	35.807350005767745	38.0	34.0	39.0	30.0	40.0
27	35.651735507262245	38.0	34.0	39.0	30.0	40.0
28	35.364457985260856	37.0	34.0	39.0	30.0	40.0
29	35.94858091562592	38.0	34.0	40.0	30.0	40.0
30	36.26178335764548	38.0	34.0	40.0	31.0	40.0
31	36.43042419941505	38.0	35.0	40.0	31.0	40.0
32	36.47722397688229	38.0	35.0	40.0	31.0	40.0
33	36.372045125671136	38.0	35.0	40.0	31.0	40.0
34	35.718953103119986	38.0	34.0	40.0	28.0	40.0
35	35.80915625917181	38.0	34.0	40.0	29.0	40.0
36	35.9465570941077	38.0	34.0	40.0	30.0	40.0
37	35.9897918208991	38.0	34.0	40.0	30.0	40.0
38	35.94267007182668	38.0	34.0	40.0	30.0	40.0
39	35.83443869616832	38.0	34.0	40.0	30.0	40.0
40	35.695659443113236	38.0	34.0	40.0	30.0	40.0
41	35.561514828946194	38.0	34.0	40.0	30.0	40.0
42	35.47966468227024	37.0	34.0	40.0	29.0	40.0
43	35.430012426205714	37.0	34.0	40.0	29.0	40.0
44	35.14641238940896	37.0	34.0	40.0	29.0	40.0
45	35.08946795104863	37.0	34.0	40.0	28.0	40.0
46	34.84655242150391	36.0	33.0	40.0	28.0	40.0
47	34.732074358648624	36.0	33.0	40.0	27.0	40.0
48	34.622690164023	36.0	33.0	40.0	27.0	40.0
49	34.56644953660912	36.0	33.0	40.0	27.0	40.0
50	33.94546633315957	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	8.0
15	29.0
16	68.0
17	135.0
18	297.0
19	529.0
20	856.0
21	1316.0
22	2082.0
23	3121.0
24	4670.0
25	6724.0
26	9383.0
27	13115.0
28	18145.0
29	23558.0
30	31334.0
31	44670.0
32	67000.0
33	98025.0
34	138484.0
35	112752.0
36	175640.0
37	274065.0
38	331974.0
39	11704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.19507098707295	0.6546025877463886	0.09651847211696696	0.05380795306369489
8	99.29085936484712	0.6175868045668861	0.04906233983555355	0.04249149075043477
9	98.18980408648406	1.046371212088026	0.3327039920098475	0.431120709418071
10	56.927646190440726	30.366521961967923	4.397212207761487	8.308619639829859
11	38.25519133582442	22.852463995397486	21.260493280941763	17.631851387836335
12	34.999262604713785	16.454793288388725	25.28491931727418	23.261024789623317
13	26.868420937353527	16.38309802392665	28.10768307480693	28.640797963912895
14	24.965211004566008	16.86444922412874	32.52614102794363	25.644198743361617
15	29.092361314929114	18.17986020153524	28.610572058121353	24.11720642541429
16	34.46315432880237	18.93068922366148	24.982587249924435	21.623569197611715
17	31.919213600781493	19.660564538149618	24.726251126170524	23.693970734898365
18	31.97740211990193	21.379936715422367	24.421071690883895	22.221589473791802
19	29.875387497572437	24.34039626600549	22.47427512583176	23.30994111059031
20	31.189557314596193	24.150790765182677	22.305696342081323	22.353955578139807
21	30.750624595710256	24.621701616282856	23.859848169580474	20.767825618426414
22	27.91836961172123	21.642113593918605	28.22749155645893	22.21202523790124
23	29.99242162072183	20.728181495612862	26.745692078330364	22.533704805334946
24	32.4850367164445	20.53974414573851	24.150352708577003	22.824866429239986
25	29.310075447949384	20.346853220373138	22.742730815676	27.600340516001477
26	25.02412961802924	20.95765014755207	26.437081199632615	27.581139034786073
27	24.063544491219155	19.880249925895424	26.240539802553286	29.815665780332136
28	24.969007495148524	25.17401798660423	22.68768170222956	27.169292816017688
29	29.522678920570115	24.2848360865191	22.1974963604797	23.994988632431085
30	28.755130737993962	21.055190751748942	26.116642792581658	24.073035717675438
31	31.67178462800963	21.27531419610042	23.461581705587996	23.591319470301954
32	34.70525361287186	21.119731091651666	22.82150799526315	21.353507300213334
33	34.400731262493736	20.83762263759723	23.49589613969917	21.26574996020986
34	28.186168216656952	22.67738737199621	29.087469682832417	20.04897472851442
35	25.47175045959439	27.527477100590936	27.08569701376812	19.915075426046556
36	25.703190366259125	30.49282828327076	22.61876079627009	21.18522055420001
37	29.707027742124836	28.168353914692858	21.40315371552312	20.721464627659188
38	29.241154541989918	29.030522324094722	20.85638606220696	20.87193707170841
39	30.563720443955766	25.707570932315875	20.730298769206957	22.9984098545214
40	27.928517923086023	23.695357914149668	21.331531460495327	27.04459270226899
41	23.535978319118396	26.868566956222082	22.163327945237082	27.43212677942244
42	22.066663454251557	24.285858218599007	22.722872249552086	30.92460607759735
43	23.047107147185557	21.949191274496492	24.40617776629096	30.59752381202699
44	24.63520836162449	20.62881565555901	23.60212486657526	31.13385111624124
45	28.55428178429217	22.407617512334944	22.912185712637786	26.125914990735104
46	25.84468264989202	25.97675671650291	22.97176141100953	25.206799222595542
47	23.685866687693384	23.95753479264591	24.208249189960327	28.148349329700384
48	25.499055988014774	22.77755631582713	27.626258865170556	24.09712883098754
49	28.602760048653487	21.898303698803957	25.006826382105096	24.49210987043746
50	24.605639540741457	21.293493545235915	26.619093719290404	27.481773194732224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	4.0
10	4.0
11	41.0
12	78.0
13	485.0
14	892.0
15	1614.0
16	2336.0
17	2990.5
18	3645.0
19	3140.5
20	2636.0
21	3047.0
22	3458.0
23	3660.5
24	3863.0
25	4081.5
26	4300.0
27	4232.5
28	4165.0
29	5756.5
30	7348.0
31	8852.5
32	10357.0
33	12564.5
34	14772.0
35	15859.5
36	16947.0
37	19546.5
38	22146.0
39	24227.5
40	26309.0
41	28150.5
42	29992.0
43	32166.0
44	34340.0
45	38284.0
46	42228.0
47	41830.0
48	41432.0
49	46796.0
50	52160.0
51	57821.5
52	63483.0
53	66774.5
54	70066.0
55	73503.5
56	76941.0
57	81539.5
58	86138.0
59	102806.0
60	119474.0
61	123535.0
62	127596.0
63	137744.5
64	147893.0
65	141515.5
66	135138.0
67	108702.5
68	82267.0
69	65627.0
70	48987.0
71	38262.5
72	27538.0
73	24577.0
74	21616.0
75	17630.0
76	13644.0
77	14245.0
78	14846.0
79	10330.5
80	5815.0
81	4463.5
82	3112.0
83	2166.5
84	1221.0
85	798.5
86	376.0
87	228.0
88	80.0
89	51.5
90	23.0
91	15.5
92	8.0
93	7.5
94	7.0
95	6.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1369686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.428071768294567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.35020244567927	5.507761962847639
2	9.56643250188633	1.6125315938488747
3	3.761924677379235	0.9511731350361171
4	1.870336621597762	0.6305332511078214
5	1.033214873531904	0.435400455309814
6	0.7029866027941586	0.35548929242992516
7	0.5567680855725679	0.3284736968451101
8	0.44471142192034663	0.29984478241000057
9	0.3641727219900299	0.2762346453288441
>10	10.136429987435278	24.349664586155296
>50	4.208119773065286	24.27344920671324
>100	1.900525905413925	27.55845673716112
>500	0.06653153791407568	3.770926576255304
>1k	0.03326576895703784	4.896006978550689
>5k	0.0017508299451072546	1.1140870925739599
>10k+	0.002626244917660882	3.6399660074262457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	24887	1.8169857909039004	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	12884	0.9406535512518928	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	11564	0.844281098003484	No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	8051	0.5877989553810143	No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	7049	0.5146435022333586	No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4989	0.36424406761841766	No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4115	0.3004338220584864	No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4072	0.2972944163844852	No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3297	0.2407121048181846	No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	2724	0.19887769897626173	No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2460	0.17960320832657994	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	2376	0.17347041584713577	No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2307	0.16843276488187803	No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGTGGGGAAGAGCACCAACTGT	2275	0.16609646298494693	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	2203	0.1608397837168519	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTA	2057	0.15018040631210364	No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	1793	0.1309059156624219	No Hit
GTCGGTGGGAGAGGCCTTCACAGAGGGCCTCAATTCTACTCTCTGGTTTT	1691	0.12345895336595394	No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1570	0.11462481181818314	No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACC	1527	0.11148540614418195	No Hit
GTCGGTGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAGCACTGACTGC	1507	0.11002521745860001	No Hit
GTCGGTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTG	1503	0.10973317972148361	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0029203773711639017	0.0	0.0	0.0	0.0
8	0.004015518885350365	0.0	0.0	0.0	0.0
9	0.004964641530978633	0.0	0.0	0.0	0.0
10	0.018106339701216192	0.0	0.0	0.0	0.0
11	0.02832766050028985	0.0	0.0	0.0	0.0
12	0.03635869827099058	0.0	0.0	0.0	0.0
13	0.04336760396178394	0.0	0.0	0.0	0.0
14	0.048989330401274456	0.0	0.0	0.0	0.0
15	0.05599823609206782	0.0	0.0	0.0	0.0
16	0.06475936820555953	0.0	0.0	0.0	0.0
17	0.07322846258193484	0.0	0.0	0.0	0.0
18	0.08220862299826384	0.0	0.0	0.0	0.0
19	0.09425517965431493	0.0	0.0	0.0	0.0
20	0.10527960423045866	0.0	0.0	0.0	0.0
21	0.11418675521250857	0.0	0.0	0.0	0.0
22	0.121998764680372	0.0	0.0	0.0	0.0
23	0.12988378358251454	0.0	0.0	0.0	0.0
24	0.14127325533005375	0.0	0.0	0.0	0.0
25	0.1490122553636381	0.0	0.0	0.0	0.0
26	0.157919406345688	0.0	0.0	0.0	0.0
27	0.16668053845917968	0.0	0.0	0.0	0.0
28	0.17719389699536975	0.0	0.0	0.0	0.0
29	0.1872691989258852	0.0	0.0	0.0	0.0
30	0.19727149142212155	0.0	0.0	0.0	0.0
31	0.20690873674696245	0.0	0.0	0.0	0.0
32	0.21800617075738526	0.0	0.0	0.0	0.0
33	0.22764341608222616	0.0	0.0	0.0	0.0
34	0.2383758029212535	0.0	0.0	0.0	0.0
35	0.2502033312744673	0.0	0.0	0.0	0.0
36	0.2659003596444733	0.0	0.0	0.0	0.0
37	0.2786040012090362	0.0	0.0	0.0	0.0
38	0.2903585201279709	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	20	7.857012E-4	44.0	34
ATTACGA	40	8.314601E-9	44.0	39
TCGATAG	20	7.857012E-4	44.0	36
TCCCGTA	20	7.857012E-4	44.0	13
ACCGTAA	35	1.4463512E-7	44.0	29
ATACGTT	25	4.4432876E-5	44.0	10
CGATATT	25	4.4432876E-5	44.0	21
TACGTAC	35	1.4463512E-7	44.0	27
GTCGGTA	935	0.0	42.82353	1
GTCGGTG	140260	0.0	42.61657	1
CGGTGGG	137230	0.0	42.545948	3
TCGGTGG	139760	0.0	42.48884	2
GGTGGGA	42305	0.0	42.169483	4
GTGGGGG	35825	0.0	41.97348	5
GGTGGGG	79425	0.0	41.95581	4
GTGGGAT	13635	0.0	41.934727	5
TGGGGGG	21445	0.0	41.671253	6
GTGGGGC	15280	0.0	41.53796	5
GTGGGAA	8490	0.0	40.9682	5
TGGGGCT	8890	0.0	40.807648	6
>>END_MODULE