FastQCFastQC Report
Fri 17 Jun 2016
SRR1527803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094244
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC387041.848113209349054No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC177740.8487072184521001No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT173940.8305622458510088No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG131780.6292485498346897No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC113590.5423914309889392No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA82180.3924089074625497No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT62350.2977208004415913No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT61020.29137006003120935No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC53070.25340886735261026No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC37250.17786848141859304No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGTGGGGAAGAGCACCAACTGTTC36140.17256823942195848No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT35450.16927349439702347No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC34860.16645624865106454No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC31850.15208352035388426No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC31020.14812027633838273No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACT30410.14520753073662857No Hit
GTCGGTGGGAGAGGCCTTCACAGAGGGCCTCAATTCTACTCTCTGGTTTTGT28790.13747204241721594No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT28700.13704229306613747No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT25350.12104606722043851No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCAT23350.11149608164091672No Hit
GTCGGTGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAGCACTGACTGCTC22880.1092518350297291No Hit
GTCGGTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT21920.10466784195155865No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCTC21160.10103884743134037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA253.4181245E-546.046
CGGCGTA206.312649E-446.020
CGAACTA206.312649E-446.042
TTATGCG206.312649E-446.018
ACCGATA405.6152203E-946.043
TAGTTCG253.4181245E-546.031
CGTTAGC206.312649E-446.016
CGTTAGA206.312649E-446.011
TACGCTT206.312649E-446.040
CCGTAGA301.8621413E-646.045
TACGACT206.312649E-446.041
CGTACGA206.312649E-446.046
ATGCGAT206.312649E-446.039
TTACGCC206.312649E-446.025
ATAACCG253.4181245E-546.044
ATACGTT206.312649E-446.010
CCACGTA206.312649E-446.018
CGTAATC351.02010745E-745.99999614
TATAACG351.02010745E-745.99999642
GTCGGTG2144200.044.5873071
Produced by FastQC (version 0.11.5)