FastQCFastQC Report
Fri 17 Jun 2016
SRR1527802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108639
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC364861.7303104040094108No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT169540.8040257246498808No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC164060.7780373975820423No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG126130.5981583381508168No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC108800.5159726249965025No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA76890.36464278617629664No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT60780.2882427954713917No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT60540.2871046205633112No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC49800.23617129342670792No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGTGGGGAAGAGCACCAACTGTTC35330.16754883126035325No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC33800.16029296622133993No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT33680.15972387876729965No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC33090.15692586545160173No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC30190.14317291864562878No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC29780.1412285365109912No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACT28740.13629644524264228No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT26960.12785498134104512No Hit
GTCGGTGGGAGAGGCCTTCACAGAGGGCCTCAATTCTACTCTCTGGTTTTGT26340.12491469616183709No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT25690.12183213911911903No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCAT22760.10793692044963599No Hit
GTCGGTGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAGCACTGACTGCTC22040.10452239572539444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAT206.312657E-446.00000446
ACCGATA206.312657E-446.00000443
CACGATT206.312657E-446.00000432
CACTACG206.312657E-446.00000418
CGTACTC206.312657E-446.00000440
ACGTCTA206.312657E-446.00000438
ATACGTC206.312657E-446.00000439
CCGCATA206.312657E-446.00000438
GCGTTAG253.4181336E-546.032
CGTAATC253.4181336E-546.014
CGTAAGC253.4181336E-546.013
CGGTCGA301.8621486E-646.039
CGCGTTA253.4181336E-546.037
GTCGGTG2162100.044.558581
CGGTGGG2114450.044.4825823
TCGGTGG2154400.044.4157072
GGTGGGA646850.043.9945874
GGTGGGG1223950.043.9742664
GTCGGTA12650.043.8181841
GTGGGGG552850.043.7659385
Produced by FastQC (version 0.11.5)