##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527802_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2108639
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.532611319434004	31.0	31.0	33.0	30.0	33.0
2	31.329603597391493	31.0	31.0	33.0	30.0	34.0
3	32.20099694637157	33.0	31.0	34.0	31.0	34.0
4	36.259429897673336	37.0	35.0	37.0	35.0	37.0
5	36.29287137343092	37.0	37.0	37.0	35.0	37.0
6	33.20238409704079	35.0	32.0	35.0	30.0	35.0
7	35.45661964897737	35.0	35.0	37.0	35.0	37.0
8	36.416953779191225	37.0	35.0	37.0	35.0	37.0
9	38.62719981940958	39.0	39.0	39.0	37.0	39.0
10	37.885166688086485	39.0	38.0	39.0	35.0	39.0
11	37.51010438486626	39.0	37.0	39.0	35.0	39.0
12	36.691016338026564	38.0	35.0	39.0	33.0	39.0
13	36.42150885002127	38.0	35.0	39.0	33.0	39.0
14	37.20297879343026	39.0	36.0	40.0	33.0	40.0
15	37.23679539266797	39.0	35.0	40.0	33.0	40.0
16	37.294831405470546	39.0	35.0	40.0	33.0	40.0
17	37.07686379697995	39.0	35.0	40.0	32.0	40.0
18	37.09771421281689	39.0	35.0	40.0	32.0	40.0
19	37.01595009861811	39.0	35.0	40.0	32.0	40.0
20	36.96793002500665	39.0	35.0	40.0	32.0	40.0
21	37.05695522087944	39.0	35.0	40.0	32.0	40.0
22	36.944004165720166	39.0	35.0	40.0	32.0	40.0
23	36.98920725643413	39.0	35.0	40.0	32.0	40.0
24	37.03797994820356	39.0	35.0	40.0	32.0	40.0
25	36.96786173451217	38.0	35.0	40.0	32.0	40.0
26	36.7269404578024	38.0	35.0	40.0	32.0	40.0
27	36.637690472385266	38.0	35.0	40.0	31.0	40.0
28	36.517428066160214	38.0	35.0	40.0	31.0	40.0
29	36.40678798030388	38.0	35.0	40.0	31.0	40.0
30	36.22235669547988	38.0	35.0	40.0	31.0	40.0
31	36.14539093699775	38.0	34.0	40.0	31.0	40.0
32	36.09235578019756	38.0	35.0	40.0	31.0	40.0
33	35.80218804641287	38.0	35.0	40.0	30.0	40.0
34	35.35879446410694	38.0	34.0	40.0	27.0	40.0
35	35.424089187385796	38.0	34.0	40.0	28.0	40.0
36	35.42702710136728	38.0	34.0	40.0	29.0	40.0
37	35.54889148877546	38.0	34.0	40.0	29.0	40.0
38	35.22881773504142	37.0	34.0	40.0	29.0	40.0
39	35.29105930412935	37.0	34.0	40.0	29.0	40.0
40	35.19752930681828	37.0	34.0	40.0	29.0	40.0
41	34.844282022669596	37.0	34.0	40.0	28.0	40.0
42	34.887664982009724	37.0	33.0	40.0	28.0	40.0
43	34.93022229030194	36.0	33.0	39.0	28.0	40.0
44	34.808638652704424	36.0	33.0	39.0	28.0	40.0
45	34.62168061958448	36.0	33.0	39.0	27.0	40.0
46	34.153520825518264	35.0	33.0	39.0	26.0	40.0
47	33.88961363230027	35.0	33.0	39.0	24.0	40.0
48	33.86144712300209	35.0	32.0	39.0	24.0	40.0
49	33.8396600840637	35.0	32.0	39.0	26.0	40.0
50	33.76286125790142	35.0	32.0	38.0	26.0	40.0
51	34.29150983169713	35.0	33.0	39.0	27.0	40.0
52	34.08233889252736	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	3.0
15	24.0
16	65.0
17	155.0
18	417.0
19	719.0
20	1249.0
21	2254.0
22	3580.0
23	5556.0
24	8075.0
25	11674.0
26	15667.0
27	21327.0
28	28151.0
29	36940.0
30	49405.0
31	65891.0
32	89610.0
33	137146.0
34	234715.0
35	183132.0
36	262457.0
37	390483.0
38	522813.0
39	37127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.23865583440313	0.6415512565213866	0.07175244316357612	0.04804046591189862
8	99.30519164257134	0.6110576537757293	0.04462594118765706	0.03912476246526788
9	98.20277439618636	1.0074744894692738	0.33836991538143796	0.45138119896293294
10	57.099674244856516	30.0982766609173	4.447323605415626	8.354725488810555
11	38.49359705478273	22.655798360933286	21.066669069480362	17.783935514803627
12	34.99271330939056	16.4845665853662	25.21379904288975	23.30892106235349
13	27.313257508753274	16.346562877761436	27.969794734897725	28.37038487858756
14	25.36180920489472	16.56826986506462	32.41593274145077	25.653988188589892
15	29.078709063049672	18.017593338641653	28.60769434692235	24.29600325138632
16	34.53018748111934	18.721364823471443	25.000011855988625	21.748435839420594
17	32.016196228941986	19.475879939619823	24.73258817654421	23.775335654893986
18	32.08092992683906	21.260917587126105	24.432299696628963	22.225852789405867
19	30.073711052484565	24.194705684567154	22.460316820470457	23.271266442477824
20	31.254946911254134	24.00406138746367	22.326865812498013	22.414125888784188
21	30.811580360602264	24.565323889010873	23.805876681594146	20.817219068792713
22	28.25708905127905	21.53208775897629	28.081478147753124	22.12934504199154
23	30.06180763990422	20.606798982661328	26.643394151393384	22.68799922604106
24	32.4928069717007	20.357823221518714	24.209881350008228	22.939488456772352
25	29.48835718204965	20.271653896186116	22.714129824972414	27.525859096791816
26	25.107521960847734	20.83599895477604	26.40082062410873	27.655658460267503
27	24.081030465622614	19.70161796305579	26.202209102648677	30.015142468672924
28	25.033540591822494	24.981231970005297	22.717402077833142	27.26782536033906
29	29.492767609818465	24.062819667093326	22.22855595481256	24.21585676827565
30	28.92206774132509	20.844914658222674	26.061122838001193	24.17189476245104
31	31.737959887870804	21.194381778957897	23.44417418059706	23.623484152574243
32	34.78864803316262	21.063633936392144	22.75396594675523	21.39375208369
33	34.52776885943967	20.7364560742735	23.468550093211782	21.26722497307505
34	28.26477173190859	22.535531212312776	29.09616107830691	20.103535977471726
35	25.703830764772917	27.327010455559247	27.04896381030608	19.920194969361756
36	25.92307170644193	30.329515863075663	22.56920222001016	21.178210210472255
37	29.6826531236499	28.149104706874912	21.397925391686297	20.770316777788896
38	29.348171972537735	28.832341619404744	20.813425152432448	21.006061255625074
39	30.69515455229653	25.619416125756945	20.623492214646507	23.061937107300018
40	27.955425276683204	23.57307248893718	21.30118052449945	27.170321709880163
41	23.91461980927034	26.594405206391418	22.16619345464065	27.32478152969759
42	22.192608597299017	24.09317099797547	22.628814130820878	31.08540627390464
43	23.006925320076125	21.900050221967817	24.413804354372655	30.6792201035834
44	24.48825996294292	20.481789438590482	23.60973120576827	31.42021939269832
45	28.57046654263722	22.308417894196207	22.86436891283904	26.256746650327536
46	25.94161447265274	25.804701516001554	22.999195215492076	25.25448879585363
47	24.065712528318027	23.803600331777986	24.18446211039443	27.94622502950956
48	25.6682627988954	22.602920651662043	27.623505019114226	24.10531153032833
49	28.58488342480624	21.70257687541585	25.120610972290656	24.591928727487257
50	24.572579754049887	21.08729848968932	26.673366090639504	27.666755665621285
51	22.95087020585316	22.268771468231403	27.69592139764085	27.08443692827459
52	23.20971014953247	22.419864187279092	27.269437774792177	27.10098788839626
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.5
8	9.0
9	11.5
10	14.0
11	57.0
12	100.0
13	591.5
14	2147.0
15	3211.0
16	4061.0
17	4911.0
18	4287.0
19	3663.0
20	4221.0
21	4779.0
22	5061.5
23	5344.0
24	5095.5
25	4847.0
26	5454.0
27	6061.0
28	6635.0
29	7209.0
30	9916.5
31	12624.0
32	15943.0
33	19262.0
34	20811.5
35	22361.0
36	24095.0
37	25829.0
38	29711.0
39	39003.5
40	44414.0
41	45460.5
42	46507.0
43	48259.0
44	50011.0
45	55088.5
46	60166.0
47	64204.5
48	68243.0
49	72340.5
50	76438.0
51	83940.0
52	91442.0
53	97917.5
54	104393.0
55	108016.5
56	111640.0
57	121936.0
58	132232.0
59	152818.5
60	173405.0
61	187413.0
62	201421.0
63	208722.0
64	210793.0
65	205563.0
66	166197.5
67	126832.0
68	106888.0
69	86944.0
70	69647.0
71	52350.0
72	42843.5
73	33337.0
74	28826.5
75	24316.0
76	24562.5
77	24809.0
78	19240.5
79	13672.0
80	9422.5
81	5173.0
82	4262.5
83	3352.0
84	2057.0
85	762.0
86	502.5
87	243.0
88	135.5
89	19.0
90	10.0
91	8.0
92	6.0
93	5.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2108639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.871698844866136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.03327972588703	5.276657905858535
2	10.157052653228043	1.5990651927531938
3	4.455189374190946	1.0520972715143624
4	2.2645019976678453	0.7130191103695613
5	1.270217698297522	0.4999385594208563
6	0.7835971120454128	0.3700944289036989
7	0.5334251187025019	0.2939273323499145
8	0.3845399155491909	0.242158592722679
9	0.328217326038972	0.23252651516214246
>10	5.727536694975994	12.782038794736794
>50	4.314187288977866	24.263364184193787
>100	2.6052677218882256	36.2841767738673
>500	0.09448504794082055	4.984565894283389
>1k	0.043463122052777456	5.795460278286954
>5k	0.001889700958816411	0.9828016133872257
>10k+	0.003149501598027352	4.6281075521896105
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	36486	1.7303104040094108	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	16954	0.8040257246498808	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	16406	0.7780373975820423	No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	12613	0.5981583381508168	No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10880	0.5159726249965025	No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7689	0.36464278617629664	No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6078	0.2882427954713917	No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6054	0.2871046205633112	No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4980	0.23617129342670792	No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGTGGGGAAGAGCACCAACTGTTC	3533	0.16754883126035325	No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3380	0.16029296622133993	No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3368	0.15972387876729965	No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	3309	0.15692586545160173	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	3019	0.14317291864562878	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2978	0.1412285365109912	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACT	2874	0.13629644524264228	No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	2696	0.12785498134104512	No Hit
GTCGGTGGGAGAGGCCTTCACAGAGGGCCTCAATTCTACTCTCTGGTTTTGT	2634	0.12491469616183709	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	2569	0.12183213911911903	No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCAT	2276	0.10793692044963599	No Hit
GTCGGTGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAGCACTGACTGCTC	2204	0.10452239572539444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0035567965877516257	0.0	0.0	0.0	0.0
8	0.0044104277688120155	0.0	0.0	0.0	0.0
9	0.0056908745404026006	0.0	0.0	0.0	0.0
10	0.01787883084776484	0.0	0.0	0.0	0.0
11	0.027505893611945904	0.0	0.0	0.0	0.0
12	0.03599478146804645	0.0	0.0	0.0	0.0
13	0.04301352673454299	0.0	0.0	0.0	0.0
14	0.04823016172991204	0.0	0.0	0.0	0.0
15	0.05496436326938845	0.0	0.0	0.0	0.0
16	0.06350067507999235	0.0	0.0	0.0	0.0
17	0.07194213898158955	0.0	0.0	0.0	0.0
18	0.08005163520166325	0.0	0.0	0.0	0.0
19	0.09186019987299865	0.0	0.0	0.0	0.0
20	0.10096559913764282	0.0	0.0	0.0	0.0
21	0.10902767140321316	0.0	0.0	0.0	0.0
22	0.1164732322602399	0.0	0.0	0.0	0.0
23	0.12439303266230019	0.0	0.0	0.0	0.0
24	0.13288192051840073	0.0	0.0	0.0	0.0
25	0.13994808973940062	0.0	0.0	0.0	0.0
26	0.14810500991397768	0.0	0.0	0.0	0.0
27	0.1563093540430581	0.0	0.0	0.0	0.0
28	0.1675962552148566	0.0	0.0	0.0	0.0
29	0.17575317538943366	0.0	0.0	0.0	0.0
30	0.18400494347301743	0.0	0.0	0.0	0.0
31	0.1932051906466683	0.0	0.0	0.0	0.0
32	0.20349618877389633	0.0	0.0	0.0	0.0
33	0.2117479568574801	0.0	0.0	0.0	0.0
34	0.22075850821311757	0.0	0.0	0.0	0.0
35	0.23190313752140598	0.0	0.0	0.0	0.0
36	0.24608289991790913	0.0	0.0	0.0	0.0
37	0.2578440406347412	0.0	0.0	0.0	0.0
38	0.26927321367004975	0.0	0.0	0.0	0.0
39	0.2793745159792643	0.0	0.0	0.0	0.0
40	0.288811882925432	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	20	6.312657E-4	46.000004	46
ACCGATA	20	6.312657E-4	46.000004	43
CACGATT	20	6.312657E-4	46.000004	32
CACTACG	20	6.312657E-4	46.000004	18
CGTACTC	20	6.312657E-4	46.000004	40
ACGTCTA	20	6.312657E-4	46.000004	38
ATACGTC	20	6.312657E-4	46.000004	39
CCGCATA	20	6.312657E-4	46.000004	38
GCGTTAG	25	3.4181336E-5	46.0	32
CGTAATC	25	3.4181336E-5	46.0	14
CGTAAGC	25	3.4181336E-5	46.0	13
CGGTCGA	30	1.8621486E-6	46.0	39
CGCGTTA	25	3.4181336E-5	46.0	37
GTCGGTG	216210	0.0	44.55858	1
CGGTGGG	211445	0.0	44.482582	3
TCGGTGG	215440	0.0	44.415707	2
GGTGGGA	64685	0.0	43.994587	4
GGTGGGG	122395	0.0	43.974266	4
GTCGGTA	1265	0.0	43.818184	1
GTGGGGG	55285	0.0	43.765938	5
>>END_MODULE