FastQCFastQC Report
Fri 17 Jun 2016
SRR1527801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84951
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGT7270.8557874539440383No Hit
CTGTGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT5740.6756836293863522No Hit
CTGTGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA5070.5968146343186073No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAA4670.5497286671139834No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGG4410.5191227884309779No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAG4150.4885169097479724No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAA4070.47909971630704756No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT3950.46497392614566047No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTG3790.44613953926381084No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.42141940648138343No Hit
CTGTGTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC3190.3755105884568751No Hit
CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.3566762015750256No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC2770.3260703228920201No Hit
CTGTGTGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG2770.3260703228920201No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAA2580.3037044884698238No Hit
CTGTGTGGGAGGATGGACTTTCTGGACGTGAGGATAGAGTCTTTGTGCCC2570.3025273392897082No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC2560.3013501901095926No Hit
CTGTGTGGGTGCTCTGTGTGGGGAATGCTGTACATCTATTTGATTGTAAA2410.28369295240785863No Hit
CTGTGTGGGAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCGGAG2380.28016150486751185No Hit
CTGTGTGGATTCGAACTAACCAACCAGCTCGCTTCTCGCTTCTGTAACCA2320.27309860978681827No Hit
CTGTGTGGGGAGAAAATGTACGTGCTTTTCAGAGGGGCTCTGAACAGAAT2260.26603571470612475No Hit
CTGTGTGGGTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGA2200.2589728196254311No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAA2150.2530870737248532No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCCCTGACTGAG2030.23896128356346602No Hit
CTGTGTGGGACTCGTTTGGTAAATAATATTGCCTGTTCTCACTATAGGCC2020.23778413438335042No Hit
CTGTGTGGGTTACTATTCCTCAAAAAACCAAAAGCGCGTTATTTACCCAG1960.2307212393026568No Hit
CTGTGTGGGATACCAGAAATTCCTGATGACCTGAAGCAACTCTATAAGAC1940.22836694094242566No Hit
CTGTGTGGGTGGAGTGCAGGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTG1900.22365834422196323No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGGGGGAAAAAAAAAAAAAAAAAAAA1870.22012689668161645No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGG1870.22012689668161645No Hit
CTGTGTGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAG1820.21424115078103848No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC1820.21424115078103848No Hit
CTGTGTGGGTGCAGTGCTCTGTGTGGGGAGTTCTCGCCGGAGCTTGGGGA1810.21306400160092287No Hit
CTGTGTGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGG1800.21188685242080732No Hit
CTGTGTGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCACT1790.21070970324069171No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTG1790.21070970324069171No Hit
CTGTGTGGGGATGCCTCGCCTTTCTGTACTCATAGCGGGGGTAAAATGTT1770.2083554048804605No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA1740.20482395734011372No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGCTGGTGAGATGGCTCAGTGG1730.2036468081599981No Hit
CTGTGTGGGTAACCAGACTCAAAAAGAAAACCTCAGGAAATTCTACAAGG1730.2036468081599981No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCT1710.2012925097997669No Hit
CTGTGTGGGGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1700.20011536061965135No Hit
CTGTGTGGGGCAGACCTGCCGCGAGGAGCACAAGAAGAAGCATCCCGACT1700.20011536061965135No Hit
CTGTGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA1700.20011536061965135No Hit
CTGTGTGGGGTGCAGTGCCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGCA1700.20011536061965135No Hit
CTGTGTGGGGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATT1690.19893821143953574No Hit
CTGTGTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA1670.19658391307930453No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAGTGCAGTGCTCTGTGTGGG1630.19187531635884214No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGTAAAAAAAAAAAAAAAAA1620.19069816717872656No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGG1600.18834386881849538No Hit
CTGTGTGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAAGC1590.18716671963837977No Hit
CTGTGTGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTG1570.1848124212781486No Hit
CTGTGTGGGTTTCTGCGGCGGTAGCCTTACTGGAGCGACGCAGTCAGGCG1570.1848124212781486No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGCAAAAAAAAAAAAAAAA1570.1848124212781486No Hit
CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.183635272098033No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGAAAAAAAAAAAAAAAAA1550.18245812291791738No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGT1490.1753952278372238No Hit
CTGTGTGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCA1460.17186378029687704No Hit
CTGTGTGGGGAAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGT1440.16950948193664583No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAGCTGGAGAGATGGCTCA1440.16950948193664583No Hit
CTGTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1430.16833233275653023Illumina Single End Adapter 2 (97% over 34bp)
CTGTGTGGGGCTTTTCTGCGCTCTCGCTGGACAAGTGAACAGGGACGGCC1430.16833233275653023No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCGCACCCGACTGC1430.16833233275653023No Hit
CTGTGTGGGAGGGGCGGTTCGAACCGTGCGGGCGTGGGTGCGGGCACTCG1420.16715518357641462No Hit
CTGTGTGGGAGGGAGCATTTGACCAAGAGATGTGGTTTGTGAATTCAAGG1410.16597803439629905No Hit
CTGTGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGC1410.16597803439629905No Hit
CTGTGTGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTG1370.16126943767583665No Hit
CTGTGTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA1370.16126943767583665No Hit
CTGTGTGGGGTGCTCTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTA1370.16126943767583665No Hit
CTGTGTGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTC1350.15891513931560547No Hit
CTGTGTGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCT1340.15773799013548986No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA1340.15773799013548986No Hit
CTGTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA1330.15656084095537426Illumina Single End Adapter 2 (100% over 34bp)
CTGTGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG1320.15538369177525868No Hit
CTGTGTGGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCAGTGACTGC1310.15420654259514308No Hit
CTGTGTGGGGCCTTCTTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTC1300.15302939341502747No Hit
CTGTGTGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCA1300.15302939341502747No Hit
CTGTGTGGGTCCCTACTTCCTCTCCGTGCCTCCGCCTCCGTGGAGCTCAG1290.1518522442349119No Hit
CTGTGTGGGACCTTGGATATGGTCTATTTAACAATTGGGTCATGCACATG1290.1518522442349119No Hit
CTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAATGTTTATG1290.1518522442349119No Hit
CTGTGTGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCA1280.1506750950547963No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAA1270.1494979458746807No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCAAAAAAAAAAAAAAAAAA1260.1483207966945651No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGCAGAGTGCAGTGCTCTGTGT1240.14596649833433392No Hit
CTGTGTGGGGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGTTAA1230.14478934915421832No Hit
CTGTGTGGGGCTTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCC1230.14478934915421832No Hit
CTGTGTGGGGAGTGCAGTGTCTGTGTGGGAGCAGTGGTATCAAGTGCTCT1220.1436121999741027No Hit
CTGTGTGGGAGTCTTGGGTGAAATGTGGATCTGAGTAATAAGTAGCTTCT1220.1436121999741027No Hit
CTGTGTGGGGTAGACTGGGCGCGAAGCGGTCTTCAGGTTTGGTCTCAGTT1200.14125790161387153No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG1190.14008075243375592No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAA1190.14008075243375592No Hit
CTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT1160.13654930489340913No Hit
CTGTGTGGGTGGGAGGCCGGGGAGCGTTTCCGGTGCGGGCAGTCGCAGCC1160.13654930489340913No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA1150.13537215571329356No Hit
CTGTGTGGGGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCGTTGG1140.13419500653317795No Hit
CTGTGTGGGGTCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACG1140.13419500653317795No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGAAAGAGCACCCGACTG1130.13301785735306237No Hit
CTGTGTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTC1120.13184070817294674No Hit
CTGTGTGGGGGGGCTGGAGAGGTGGCTCAGAGGTTAAGAGCACTGACTGC1120.13184070817294674No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAGTG1110.13066355899283116No Hit
CTGTGTGGGGGGGCTGGAGTAATGGCTCAGTGGTTAAGAGCACCGACTGC1110.13066355899283116No Hit
CTGTGTGGGAGTTGAGCCCCTAGCCACTCAACTTCCTTTCCTGGGACCTG1100.12948640981271556No Hit
CTGTGTGGGATTTAAAAGCTGACAGCTTTTAATATGCTTACAAAATAGTA1090.12830926063259995No Hit
CTGTGTGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTG1090.12830926063259995No Hit
CTGTGTGGGAGTGTGGTTTTCACCTCCAGTGTAAAGATGAACTGTAAATA1080.12713211145248438No Hit
CTGTGTGGGAGTGGAAGGCGAGGGGGAGGAAGAAGGAGAGGAGTACTAAG1070.12595496227236877No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAA1070.12595496227236877No Hit
CTGTGTGGGGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.12477781309225319No Hit
CTGTGTGGGATCCGCAAGCCTGTGACTGTCCATTCCCGGGCTCGTTGCCG1060.12477781309225319No Hit
CTGTGTGGGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGC1060.12477781309225319No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGGAAAAAAAAAAAAAAAA1050.12360066391213759No Hit
CTGTGTGGAGAGTGCAGTGCTCTGTGTGGGCTCTTTCCTTTTGCGGCCAT1040.122423514732022No Hit
CTGTGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG1040.122423514732022No Hit
CTGTGTGGGAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAG1030.12124636555190639No Hit
CTGTGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAA1020.12006921637179079No Hit
CTGTGTGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC1000.1177149180115596No Hit
CTGTGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA1000.1177149180115596No Hit
CTGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGA990.11653776883144401No Hit
CTGTGTGGGGCAGGAGATGATAGACATTGGGACATTGGGGACTGAGGAGC990.11653776883144401No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGTACACACACGTGGCAGACAA990.11653776883144401No Hit
CTGTGTGGGGAGTGCAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA980.1153606196513284Illumina Single End Adapter 1 (96% over 28bp)
CTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA980.1153606196513284No Hit
CTGTGTGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT980.1153606196513284No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAA960.11300632129109722No Hit
CTGTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA960.11300632129109722Illumina Single End Adapter 2 (97% over 34bp)
CTGTGTGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT930.10947487375075043No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAA920.10829772457063484No Hit
CTGTGTGGGAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGC920.10829772457063484No Hit
CTGTGTGGGATCCCACGACAGTGATCACAGTCTGCATCCTAGCCTCTCCC920.10829772457063484No Hit
CTGTGTGGGACCGTGTATGCCTTCTCCGCGCGCCCGCTGACGGGCGGGGA920.10829772457063484No Hit
CTGTGTGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGTGT920.10829772457063484No Hit
CTGTGTGGGGAGTGCAGTGTTCTGTGGGGGAGTGCAGTGCTCTGTGTGGG920.10829772457063484No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGTAAAAAAAAAAAAAAAA910.10712057539051924No Hit
CTGTGTGGGAGACACTGAAGGGGCAGTGCTTCCAGGTGGGAGCGAGGTGA910.10712057539051924No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAA910.10712057539051924No Hit
CTGTGTGGGGGCTGGTGAGATGGGTCAGTGGGTAAGAGCACCCGACTGCT900.10594342621040366No Hit
CTGTGTGGGGTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGG900.10594342621040366No Hit
CTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCAC890.10476627703028805No Hit
CTGTGTGGGCACAGTGACCCTAAAGATGAGGGGGACGCTTGTCCTCTCTG890.10476627703028805No Hit
CTGTGTGGGGGGGGAGGGAGTCAGGTCTCGTTGCACATGGTGACTCCCAA880.10358912785017245No Hit
CTGTGTGGGATTTTTGGTATTTTCCAACAACGCCCTTCCCACCTCCTTGA880.10358912785017245No Hit
CTGTGTGGGACTCGCTCAACATCAGCCTGGGATTCCGGGCTTAGCCTGAG870.10241197867005686No Hit
CTGTGTGGGGAGCCCGGCCTGTGAGCGTTCTGGATCCGGCTGCAAGATGA870.10241197867005686No Hit
CTGTGTGGGGCTTTTTGCCCCATCTACTGCGAGGATGAAGACCATTCTCA860.10123482948994125No Hit
CTGTGTGGGGCTTCCGGTCGCGAGCACCCCAGGGTGTAGAGGGCGGTCGC860.10123482948994125No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGGTAAAAAAAAAAAAAAAAAAAAAA850.10005768030982568No Hit
CTGTGTGGGAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCGACTG850.10005768030982568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTCA650.044.00000436
CAACCCC650.044.00000443
GAAGTGT650.044.00000444
CGTAGAT650.044.00000425
ACTCACA650.044.00000438
GCAGCGT650.044.00000421
TGGAAGT650.044.00000442
GCGTAGA650.044.00000424
CAGCGTA650.044.00000422
AGCGTAG650.044.00000423
TCACAAC650.044.00000440
TAGATCT650.044.00000427
ACAACCC650.044.00000442
AGGGGTT254.40801E-544.026
CAGCTCG302.5000354E-644.025
TGGTCTC207.815331E-444.040
AGCCCCT207.815331E-444.015
TGGTTTG207.815331E-444.033
CTTTTAA207.815331E-444.026
CGCTGCT302.5000354E-644.020
Produced by FastQC (version 0.11.5)