FastQCFastQC Report
Fri 17 Jun 2016
SRR1527800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125160
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGT10700.8549057206775328No Hit
CTGTGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT8940.7142857142857143No Hit
CTGTGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA7600.6072227548737615No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAGTG6200.4953659316075424No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA6190.49456695429849795No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT5870.46899968040907636No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTT5840.46660274848194305No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5370.4290508149568552No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA5290.42265899648449984No Hit
CTGTGTGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT4880.3899009268136785No Hit
CTGTGTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC4700.3755193352508789No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC4210.33636944710770217No Hit
CTGTGTGGGTGCTCTGTGTGGGGAATGCTGTACATCTATTTGATTGTAAATT3980.31799296899968044No Hit
CTGTGTGGGAGGATGGACTTTCTGGACGTGAGGATAGAGTCTTTGTGCCCAG3900.31160115052732507No Hit
CTGTGTGGGACTCGTTTGGTAAATAATATTGCCTGTTCTCACTATAGGCCTA3650.2916267178012144No Hit
CTGTGTGGGGAGAAAATGTACGTGCTTTTCAGAGGGGCTCTGAACAGAATGA3620.28922978587408116No Hit
CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3510.28044103547459254No Hit
CTGTGTGGATTCGAACTAACCAACCAGCTCGCTTCTCGCTTCTGTAACCACG3320.2652604666027485No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCCCTGACTGAGAC3250.25966762543943755No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGG3250.25966762543943755No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3220.25727069351230425No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC3200.2556727388942154No Hit
CTGTGTGGGAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCGGAGGT3140.2508788750399489No Hit
CTGTGTGGGTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGAGG3110.24848194311281557No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCT3090.24688398849472673No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAA3070.2452860338766379No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGCTGGTGAGATGGCTCAGTGGGT3060.2444870565675935No Hit
CTGTGTGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGAA2990.23889421540428252No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC2970.23729626078619367No Hit
CTGTGTGGGTGCAGTGCTCTGTGTGGGGAGTTCTCGCCGGAGCTTGGGGAAG2960.23649728347714924No Hit
CTGTGTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG2840.22690955576861616No Hit
CTGTGTGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCACTTC2740.21891978267817194No Hit
CTGTGTGGGTTACTATTCCTCAAAAAACCAAAAGCGCGTTATTTACCCAGAA2720.2173218280600831No Hit
CTGTGTGGGGATGCCTCGCCTTTCTGTACTCATAGCGGGGGTAAAATGTTGA2700.21572387344199426No Hit
CTGTGTGGGATACCAGAAATTCCTGATGACCTGAAGCAACTCTATAAGACCG2700.21572387344199426No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAA2660.21252796420581657No Hit
CTGTGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA2610.20853307766059442No Hit
CTGTGTGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAG2580.20613614573346117No Hit
CTGTGTGGGTGGAGTGCAGGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGG2570.20533716842441674No Hit
CTGTGTGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCG2560.20453819111537233No Hit
CTGTGTGGGAGGGAGCATTTGACCAAGAGATGTGGTTTGTGAATTCAAGGCA2530.20214125918823905No Hit
CTGTGTGGGGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGA2510.2005433045701502No Hit
CTGTGTGGGGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.19974432726110578No Hit
CTGTGTGGGGCAGACCTGCCGCGAGGAGCACAAGAAGAAGCATCCCGACTCG2470.19734739533397253No Hit
CTGTGTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG2460.19654841802492806No Hit
CTGTGTGGGTTTCTGCGGCGGTAGCCTTACTGGAGCGACGCAGTCAGGCGAT2410.192553531479706No Hit
CTGTGTGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAAGCTT2400.19175455417066153No Hit
CTGTGTGGGTAACCAGACTCAAAAAGAAAACCTCAGGAAATTCTACAAGGGC2380.1901565995525727No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGG2360.18855864493448385No Hit
CTGTGTGGGGCTTTTCTGCGCTCTCGCTGGACAAGTGAACAGGGACGGCCTC2350.18775966762543944No Hit
CTGTGTGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA2320.18536273569830616No Hit
CTGTGTGGGGTGCAGTGCCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGCAGA2310.18456375838926176No Hit
CTGTGTGGGTCCCTACTTCCTCTCCGTGCCTCCGCCTCCGTGGAGCTCAGTC2300.18376478108021732No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTCT2280.18216682646212848No Hit
CTGTGTGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTT2270.18136784915308404No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGTAAAAAAAAAAAAAAAAAAA2230.17817193991690636No Hit
CTGTGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA2200.1757750079897731No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAGCTGGAGAGATGGCTCAGT2190.17497603068072867No Hit
CTGTGTGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCG2170.1733780760626398No Hit
CTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC2140.17098114413550655No Hit
CTGTGTGGGGCTTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAG2140.17098114413550655No Hit
CTGTGTGGGAGGGGCGGTTCGAACCGTGCGGGCGTGGGTGCGGGCACTCGTG2080.16618728028124002No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGAAAAAAAAAAAAAAAAAAA2050.16379034835410675No Hit
CTGTGTGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCA2030.1621923937360179No Hit
CTGTGTGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGC2020.16139341642697347No Hit
CTGTGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTC2000.15979546180888463No Hit
CTGTGTGGGGCCTTCTTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAG2000.15979546180888463No Hit
CTGTGTGGGGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGA1980.15819750719079578No Hit
CTGTGTGGGGTAGACTGGGCGCGAAGCGGTCTTCAGGTTTGGTCTCAGTTGC1980.15819750719079578No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT1970.15739852988175138No Hit
CTGTGTGGGAGTGTGGTTTTCACCTCCAGTGTAAAGATGAACTGTAAATACT1940.15500159795461807No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCGCACCCGACTGCTC1930.15420262064557366No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGCAGAGTGCAGTGCTCTGTGTGG1930.15420262064557366No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGCAAAAAAAAAAAAAAAAAA1930.15420262064557366No Hit
CTGTGTGGGTGGGAGGCCGGGGAGCGTTTCCGGTGCGGGCAGTCGCAGCCGG1930.15420262064557366No Hit
CTGTGTGGGAGTTGAGCCCCTAGCCACTCAACTTCCTTTCCTGGGACCTGGG1920.15340364333652923No Hit
CTGTGTGGGGAAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTC1910.15260466602748482No Hit
CTGTGTGGGGGGGCTGGAGAGGTGGCTCAGAGGTTAAGAGCACTGACTGCTC1900.15180568871844038No Hit
CTGTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1900.15180568871844038Illumina Single End Adapter 2 (100% over 34bp)
CTGTGTGGGAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGAT1900.15180568871844038No Hit
CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.14940875679130713No Hit
CTGTGTGGGACCTTGGATATGGTCTATTTAACAATTGGGTCATGCACATGTG1870.14940875679130713No Hit
CTGTGTGGGGTCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCC1860.1486097794822627No Hit
CTGTGTGGGGAGTGCAGTGTCTGTGTGGGAGCAGTGGTATCAAGTGCTCTGT1840.14701182486417386No Hit
CTGTGTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTT1830.14621284755512942No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA1760.1406200063918185No Hit
CTGTGTGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCT1760.1406200063918185No Hit
CTGTGTGGGATCCGCAAGCCTGTGACTGTCCATTCCCGGGCTCGTTGCCGGA1730.1382230744646852No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA1730.1382230744646852No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1730.1382230744646852No Hit
CTGTGTGGGGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCGTTGGCA1710.13662511984659634No Hit
CTGTGTGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAG1710.13662511984659634No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGAAAGAGCACCCGACTGCT1700.13582614253755193No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGG1690.1350271652285075No Hit
CTGTGTGGGATTTTTGGTATTTTCCAACAACGCCCTTCCCACCTCCTTGAGT1680.1342281879194631No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGTACACACACGTGGCAGACAAAT1670.13342921061041865No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCAAAAAAAAAAAAAAAAAAAA1650.1318312559923298No Hit
CTGTGTGGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCAGTGACTGCTC1640.1310322786832854No Hit
CTGTGTGGGGCAGGAGATGATAGACATTGGGACATTGGGGACTGAGGAGCAG1640.1310322786832854No Hit
CTGTGTGGGGGGGCTGGAGTAATGGCTCAGTGGTTAAGAGCACCGACTGCTC1630.13023330137424097No Hit
CTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA1630.13023330137424097No Hit
CTGTGTGGGAGTCTTGGGTGAAATGTGGATCTGAGTAATAAGTAGCTTCTGC1620.12943432406519656No Hit
CTGTGTGGGGAGTGAACACAATTATGTACCAAATTTAACTTGGCAAACCTTT1610.12863534675615212No Hit
CTGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACC1590.12703739213806328No Hit
CTGTGTGGAGAGTGCAGTGCTCTGTGTGGGCTCTTTCCTTTTGCGGCCATCG1590.12703739213806328No Hit
CTGTGTGGGGAAAGCACATGCCTGCCCTCCTGGCACATCACCACAGAACGCT1560.12464046021093002No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGGAAAAAAAAAAAAAAAAAA1560.12464046021093002No Hit
CTGTGTGGGATTTAAAAGCTGACAGCTTTTAATATGCTTACAAAATAGTATT1550.1238414829018856No Hit
CTGTGTGGGAGTGGAAGGCGAGGGGGAGGAAGAAGGAGAGGAGTACTAAGTC1550.1238414829018856No Hit
CTGTGTGGGGTGCTCTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG1540.12304250559284118No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT1530.12224352828379675No Hit
CTGTGTGGGAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT1530.12224352828379675No Hit
CTGTGTGGGGTTTTGTTTTATTTTTTGATTTTGAATATATATATATATATCA1510.1206455736657079No Hit
CTGTGTGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC1510.1206455736657079No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.11904761904761905No Hit
CTGTGTGGGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTG1470.1174496644295302No Hit
CTGTGTGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGTGTTG1460.11665068712048576No Hit
CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA1450.11585170981144134No Hit
CTGTGTGGGGCTGGAGAGATAGCTCAGTGGTTAAGAGCACTGACTGCTCTTC1450.11585170981144134No Hit
CTGTGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG1440.11505273250239692No Hit
CTGTGTGGGGGAAGTTCGGCCAGGGTTCCCGCTCTTGCCGCGTCTGCTCCAA1430.1142537551933525No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGGACTTCTGATTCTGACAGAC1430.1142537551933525No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAA1420.11345477788430808No Hit
CTGTGTGGGAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAA1420.11345477788430808No Hit
CTGTGTGGGGTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGT1420.11345477788430808No Hit
CTGTGTGGGGAAGACAATGGCAGGCTCGGCGTTGGCAGTTCGGGCTCGGTTC1400.11185682326621924No Hit
CTGTGTGGGACTCGCTCAACATCAGCCTGGGATTCCGGGCTTAGCCTGAGCC1390.11105784595717481No Hit
CTGTGTGGGAACCTGAGCGTGGAGAATGCCTGGGGCATCCTGCGCTGCGTCA1390.11105784595717481No Hit
CTGTGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA1380.11025886864813039No Hit
CTGTGTGGGGTAGAGGGCGTGCTTTTTGGTTTTTGGTTTTGGTTTTTTGTTT1380.11025886864813039No Hit
CTGTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1380.11025886864813039Illumina Single End Adapter 2 (97% over 34bp)
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGA1360.10866091403004155No Hit
CTGTGTGGGGGGGGAGGGAGTCAGGTCTCGTTGCACATGGTGACTCCCAAGC1340.10706295941195271No Hit
CTGTGTGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGG1340.10706295941195271No Hit
CTGTGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG1330.10626398210290829No Hit
CTGTGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGA1330.10626398210290829No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAA1320.10546500479386386No Hit
CTGTGTGGGAGACACTGAAGGGGCAGTGCTTCCAGGTGGGAGCGAGGTGACC1320.10546500479386386No Hit
CTGTGTGGGGGCTGGTGAGATGGGTCAGTGGGTAAGAGCACCCGACTGCTCT1320.10546500479386386No Hit
CTGTGTGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC1310.10466602748481944No Hit
CTGTGTGGGCTCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGT1310.10466602748481944No Hit
CTGTGTGGGCTCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTTACG1310.10466602748481944No Hit
CTGTGTGGGGTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT1300.10386705017577502No Hit
CTGTGTGGGATCCCACGACAGTGATCACAGTCTGCATCCTAGCCTCTCCCTA1300.10386705017577502No Hit
CTGTGTGGGATTGCCTGGAACTTTGAGAAGTTCCTGGTGGGCCCCGACGGTG1300.10386705017577502No Hit
CTGTGTGGGGCTCTGTGTGGGAAGGGCACCTTTGCCAGCCTCAGTGAGCTCC1290.10306807286673059No Hit
CTGTGTGGGCTTTTCACGTGTCCCAGCCGGCCAGCAGGACTCTCCTTGCAGC1260.10067114093959732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATG206.289812E-446.00000439
TGATGGT206.289812E-446.00000424
CTCGACA206.289812E-446.00000429
TGGTTTG206.289812E-446.00000433
CTACAAG206.289812E-446.00000443
AGCCTCA206.289812E-446.00000437
GAGGCGC206.289812E-446.00000432
ATCCTAC206.289812E-446.00000410
TAGCGGG405.5424607E-946.00000433
TTTTGGC206.289812E-446.00000414
TGTGAAT206.289812E-446.00000438
GAGGCAA206.289812E-446.00000433
TAGCGAG206.289812E-446.00000436
ACTGCCA206.289812E-446.00000427
GGTTGTA206.289812E-446.00000435
ACTGCAC206.289812E-446.00000443
CAAGAGG206.289812E-446.00000416
CAAGAGA206.289812E-446.00000424
AACAATT206.289812E-446.00000430
GCCTCTC206.289812E-446.00000445
Produced by FastQC (version 0.11.5)