FastQCFastQC Report
Fri 17 Jun 2016
SRR1527799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125691
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGT10160.80833154322903No Hit
CTGTGTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT8350.6643275970435433No Hit
CTGTGTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA7080.5632861541399146No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAGTG6550.5211192527706836No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTT6340.5044116126055167No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT6030.47974795331407977No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA5860.46622272079942084No Hit
CTGTGTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC4880.388253733361975No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.385071325711467No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA4620.36756808363367305No Hit
CTGTGTGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT4320.3437000262548631No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC4190.3333572013907121No Hit
CTGTGTGGGAGGATGGACTTTCTGGACGTGAGGATAGAGTCTTTGTGCCCAG3750.29835071723512424No Hit
CTGTGTGGGTGCTCTGTGTGGGGAATGCTGTACATCTATTTGATTGTAAATT3680.29278150384673524No Hit
CTGTGTGGGGAGAAAATGTACGTGCTTTTCAGAGGGGCTCTGAACAGAATGA3370.2681178445552983No Hit
CTGTGTGGGTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGAGG3320.2641398349921633No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGG3280.26095742734165533No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC3180.2530014082153853No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCT3150.25061460247750433No Hit
CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.24902339865225034No Hit
CTGTGTGGGAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCGGAGGT3090.24584099100174236No Hit
CTGTGTGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCG3070.24424978717648838No Hit
CTGTGTGGATTCGAACTAACCAACCAGCTCGCTTCTCGCTTCTGTAACCACG3050.24265858335123436No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2920.2323157584870834No Hit
CTGTGTGGGATACCAGAAATTCCTGATGACCTGAAGCAACTCTATAAGACCG2920.2323157584870834No Hit
CTGTGTGGGTTACTATTCCTCAAAAAACCAAAAGCGCGTTATTTACCCAGAA2910.23152015657445638No Hit
CTGTGTGGGACTCGTTTGGTAAATAATATTGCCTGTTCTCACTATAGGCCTA2820.22435973936081344No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGCTGGTGAGATGGCTCAGTGGGT2800.22276853553555945No Hit
CTGTGTGGGGATGCCTCGCCTTTCTGTACTCATAGCGGGGGTAAAATGTTGA2790.22197293362293244No Hit
CTGTGTGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCACTTC2780.22117733171030546No Hit
CTGTGTGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAG2720.21640372023454343No Hit
CTGTGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA2720.21640372023454343No Hit
CTGTGTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG2710.21560811832191645No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAA2700.21481251640928944No Hit
CTGTGTGGGTGGAGTGCAGGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGG2650.21083450684615448No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC2600.20685649728301947No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCCCTGACTGAGAC2590.20606089537039246No Hit
CTGTGTGGGTGCAGTGCTCTGTGTGGGGAGTTCTCGCCGGAGCTTGGGGAAG2590.20606089537039246No Hit
CTGTGTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG2580.20526529345776548No Hit
CTGTGTGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAAGCTT2510.1996960800693765No Hit
CTGTGTGGGTAACCAGACTCAAAAAGAAAACCTCAGGAAATTCTACAAGGGC2460.1957180705062415No Hit
CTGTGTGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGAA2440.1941268666809875No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGG2430.19333126476836052No Hit
CTGTGTGGGTTTCTGCGGCGGTAGCCTTACTGGAGCGACGCAGTCAGGCGAT2330.1853752456420905No Hit
CTGTGTGGGAGGGGCGGTTCGAACCGTGCGGGCGTGGGTGCGGGCACTCGTG2280.18139723607895555No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAA2280.18139723607895555No Hit
CTGTGTGGGAGGGAGCATTTGACCAAGAGATGTGGTTTGTGAATTCAAGGCA2260.17980603225370154No Hit
CTGTGTGGGTCCCTACTTCCTCTCCGTGCCTCCGCCTCCGTGGAGCTCAGTC2240.17821482842844755No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAGCTGGAGAGATGGCTCAGT2220.17662362460319356No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTCT2220.17662362460319356No Hit
CTGTGTGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA2210.17582802269056655No Hit
CTGTGTGGGGCAGACCTGCCGCGAGGAGCACAAGAAGAAGCATCCCGACTCG2200.17503242077793954No Hit
CTGTGTGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCG2180.17344121695268555No Hit
CTGTGTGGGGTGCAGTGCCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGCAGA2160.17185001312743156No Hit
CTGTGTGGGGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGA2150.17105441121480458No Hit
CTGTGTGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCA2150.17105441121480458No Hit
CTGTGTGGGGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.16707640165166956No Hit
CTGTGTGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGC2080.16548519782641558No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGTAAAAAAAAAAAAAAAAAAA2050.16309839208853458No Hit
CTGTGTGGGGGAGCTGGCGAGATGGCTCAGTGGTTAAGAGCAGTGACTGCTC2020.16071158635065358No Hit
CTGTGTGGGGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGA1990.15832478061277258No Hit
CTGTGTGGGGAAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTC1980.1575291787001456No Hit
CTGTGTGGGGCTTTTCTGCGCTCTCGCTGGACAAGTGAACAGGGACGGCCTC1960.1559379748748916No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCGCACCCGACTGCTC1960.1559379748748916No Hit
CTGTGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTC1950.1551423729622646No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGTACACACACGTGGCAGACAAAT1920.1527555672243836No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGCAGAGTGCAGTGCTCTGTGTGG1910.1519599653117566No Hit
CTGTGTGGGGTGCTCTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG1900.15116436339912961No Hit
CTGTGTGGGGGGGCTGGAGAGGTGGCTCAGAGGTTAAGAGCACTGACTGCTC1870.14877755766124862No Hit
CTGTGTGGGAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGAT1840.14639075192336762No Hit
CTGTGTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTT1830.14559515001074064No Hit
CTGTGTGGGGCCTTCTTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAG1820.14479954809811363No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT1810.14400394618548665No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGAAAAAAAAAAAAAAAAAAA1800.14320834427285964No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.14320834427285964No Hit
CTGTGTGGGTGGGAGGCCGGGGAGCGTTTCCGGTGCGGGCAGTCGCAGCCGG1800.14320834427285964No Hit
CTGTGTGGGGTAGACTGGGCGCGAAGCGGTCTTCAGGTTTGGTCTCAGTTGC1790.14241274236023263No Hit
CTGTGTGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTT1780.14161714044760562No Hit
CTGTGTGGGGCTTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAG1780.14161714044760562No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1780.14161714044760562No Hit
CTGTGTGGGAGTGGAAGGCGAGGGGGAGGAAGAAGGAGAGGAGTACTAAGTC1770.14082153853497864No Hit
CTGTGTGGAGAGTGCAGTGCTCTGTGTGGGCTCTTTCCTTTTGCGGCCATCG1770.14082153853497864No Hit
CTGTGTGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCT1770.14082153853497864No Hit
CTGTGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA1760.14002593662235163No Hit
CTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC1750.13923033470972465No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGAAAGAGCACCCGACTGCT1750.13923033470972465No Hit
CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.13843473279709764No Hit
CTGTGTGGGACCTTGGATATGGTCTATTTAACAATTGGGTCATGCACATGTG1740.13843473279709764No Hit
CTGTGTGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAG1710.13604792705921664No Hit
CTGTGTGGGGTCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCC1680.13366112132133567No Hit
CTGTGTGGGAGTTGAGCCCCTAGCCACTCAACTTCCTTTCCTGGGACCTGGG1670.13286551940870867No Hit
CTGTGTGGGAGTGTGGTTTTCACCTCCAGTGTAAAGATGAACTGTAAATACT1660.13206991749608166No Hit
CTGTGTGGGGCAGGAGATGATAGACATTGGGACATTGGGGACTGAGGAGCAG1660.13206991749608166No Hit
CTGTGTGGGGAGTGCAGTGTCTGTGTGGGAGCAGTGGTATCAAGTGCTCTGT1650.13127431558345465No Hit
CTGTGTGGGAGTCTTGGGTGAAATGTGGATCTGAGTAATAAGTAGCTTCTGC1630.12968311175820066No Hit
CTGTGTGGGATTTAAAAGCTGACAGCTTTTAATATGCTTACAAAATAGTATT1600.1272963060203197No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA1600.1272963060203197No Hit
CTGTGTGGGGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCGTTGGCA1600.1272963060203197No Hit
CTGTGTGGGATCCGCAAGCCTGTGACTGTCCATTCCCGGGCTCGTTGCCGGA1580.12570510219506567No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGCAAAAAAAAAAAAAAAAAA1580.12570510219506567No Hit
CTGTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1560.12411389836981168Illumina Single End Adapter 2 (100% over 34bp)
CTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA1560.12411389836981168No Hit
CTGTGTGGGGAGTGAACACAATTATGTACCAAATTTAACTTGGCAAACCTTT1550.12331829645718469No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGA1540.12252269454455769No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCAAAAAAAAAAAAAAAAAAAA1510.12013588880667668No Hit
CTGTGTGGGAGACACTGAAGGGGCAGTGCTTCCAGGTGGGAGCGAGGTGACC1470.1169534811561687No Hit
CTGTGTGGGGGGGCTGGAGTAATGGCTCAGTGGTTAAGAGCACCGACTGCTC1460.1161578792435417No Hit
CTGTGTGGGAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAA1430.11377107350566071No Hit
CTGTGTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC1430.11377107350566071No Hit
CTGTGTGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGTGTTG1430.11377107350566071No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGG1420.11297547159303371No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT1420.11297547159303371No Hit
CTGTGTGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC1420.11297547159303371No Hit
CTGTGTGGGGAAAGCACATGCCTGCCCTCCTGGCACATCACCACAGAACGCT1410.11217986968040672No Hit
CTGTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1400.11138426776777972Illumina Single End Adapter 2 (97% over 34bp)
CTGTGTGGGGAGCCCGGCCTGTGAGCGTTCTGGATCCGGCTGCAAGATGAGC1400.11138426776777972No Hit
CTGTGTGGGATTTTTGGTATTTTCCAACAACGCCCTTCCCACCTCCTTGAGT1400.11138426776777972No Hit
CTGTGTGGGGGCCAGTCATCCGATAGACTGAGTCGCCCGGGTACCCGTGTTC1360.10820186011727172No Hit
CTGTGTGGGCTGTGTGGGAGTGCAGTGCTCTGTGTGGGCAGAGTGCAGTGCT1360.10820186011727172No Hit
CTGTGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGA1360.10820186011727172No Hit
CTGTGTGGGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTG1360.10820186011727172No Hit
CTGTGTGGGCACAGTGACCCTAAAGATGAGGGGGACGCTTGTCCTCTCTGGG1350.10740625820464472No Hit
CTGTGTGGGACTCGCTCAACATCAGCCTGGGATTCCGGGCTTAGCCTGAGCC1340.10661065629201773No Hit
CTGTGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG1340.10661065629201773No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA1330.10581505437939073No Hit
CTGTGTGGGGCTGGAGAGATAGCTCAGTGGTTAAGAGCACTGACTGCTCTTC1320.10501945246676372No Hit
CTGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACC1310.10422385055413673No Hit
CTGTGTGGGGTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGT1310.10422385055413673No Hit
CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA1300.10342824864150973No Hit
CTGTGTGGGGTTTTGTTTTATTTTTTGATTTTGAATATATATATATATATCA1300.10342824864150973No Hit
CTGTGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA1290.10263264672888274No Hit
CTGTGTGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC1280.10183704481625575No Hit
CTGTGTGGGGCTCTGTGTGGGAAGGGCACCTTTGCCAGCCTCAGTGAGCTCC1270.10104144290362875No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAA1260.10024584099100176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTG551.8189894E-1246.00000433
TTGTTAA351.00986654E-746.00000431
ACCGGAC351.00986654E-746.00000440
TCCTCAA351.00986654E-746.00000417
AACCGTG351.00986654E-746.00000422
ACTAACC351.00986654E-746.00000415
CCTATTG351.00986654E-746.00000440
CCAACCA351.00986654E-746.00000420
CCGGAAA351.00986654E-746.00000420
GTGTTAT351.00986654E-746.00000416
CGGAGGT351.00986654E-746.00000446
CGTTATT351.00986654E-746.00000437
GGCACCC551.8189894E-1246.00000439
ATCTTCG351.00986654E-746.00000426
CCCAGAA551.8189894E-1246.00000446
GAGGATG351.00986654E-746.0000049
TTACTTC351.00986654E-746.00000444
AATTCTA351.00986654E-746.00000439
TTCGCGG351.00986654E-746.00000429
AAATTCT351.00986654E-746.00000438
Produced by FastQC (version 0.11.5)