FastQCFastQC Report
Fri 17 Jun 2016
SRR1527798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899386
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC118491.317454352191384No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT58150.646552203392092No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG57880.6435501553281905No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT53410.5938495818258234No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC47740.5308065724838945No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC32410.3603569546334944No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT32060.3564654108469556No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA23670.26317954693535367No Hit
GGGCGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGC23010.25584120722359477No Hit
GGGCGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT22250.24739099785853905No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20180.2243752960352952No Hit
GGGCGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG17150.190685645540402No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG15590.17334047894897187No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT15210.169115374266444No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGTCTG13460.14965765533374992No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG13220.1469891681658376No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT12850.1428752504486394No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGC12830.14265287651798003No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTG11880.13209011481166041No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC11690.12997756247039646No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC11270.12530770992654988No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC11230.12486296206523116No Hit
GGGCGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC10150.11285476980962567No Hit
GGGCGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC9910.11018628264171335No Hit
GGGCGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG9900.11007509567638367No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT9890.10996390871105398No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG9850.10951916084973527No Hit
GGGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGTGCCGACTG9580.1065171127858339No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9420.10473812134055902No Hit
GGGCGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG9290.10329269079127315No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG9210.10240319506863572No Hit
GGGCGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG9030.10040182969270146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC302.5271002E-644.00000414
ATACGGA600.044.00000410
TACGTAT302.5271002E-644.00000413
TCACGCG254.442065E-544.039
AACGTTA351.4456054E-744.037
TCCGATT254.442065E-544.024
TCCGATA207.8555674E-444.026
ACGTTAT351.4456054E-744.038
CCGATAG207.8555674E-444.027
TTATGCG254.442065E-544.024
GCAAACG207.8555674E-444.043
CGACAAA254.442065E-544.031
TTATAAG254.442065E-544.020
CCGTATC207.8555674E-444.044
ACGATAA207.8555674E-444.032
GTTCGTT351.4456054E-744.026
GTTCGAA254.442065E-544.029
TACGATA207.8555674E-444.031
TCGTAAG207.8555674E-444.018
AGTTATG207.8555674E-444.038
Produced by FastQC (version 0.11.5)